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Possible T4-HLA class II interaction as an essential event in antigen-specific helper T lymphocyte-dependent B cell activation.
Eur J Immunol. 1986 Sep; 16(9):1111-6.EJ

Abstract

We have previously shown that the interaction between influenza virus-specific helper T lymphocytes and B lymphocytes is HLA class II restricted (Fischer, A. et al., Eur. J. Immunol. 1985. 15: 620). In the present study, we used a panel of antibodies specific for the T4 and HLA-DR molecules to investigate the role of both structures in T-B lymphocyte interaction. Several anti-T4 monoclonal antibodies were shown to block the in vitro antibody production to A/Bangkok influenza virus while they were unable to inhibit the A/Bangkok-induced proliferation of specific helper T cell clones. Some anti-HLA-DR monoclonal antibodies inhibited the antibody production to A/Bangkok, the target of the inhibition being either monocytes or B lymphocytes. The incubation of helper T cell clones with an infra-inhibitory concentration of anti-T4 antibody and of B lymphocytes with an infra-inhibitory concentration of anti-HLA-DR antibody resulted in a mutual enhancement of inhibition. Such synergistic interactions were not observed using combinations of anti-T4 and anti-B membrane structures such as p35 or LFA-1 or anti-HLA-DR and anti-T membrane structures such as T11 or LFA-1. First, these results indicate that the T4 molecule plays an essential role in T-B cell interaction even when it is not absolutely required for T cell proliferation. Second, they strongly suggest that the T4 molecule directly interacts with HLA-DR at the B cell surface. Whether such interaction is required to enhance the stability of T3/T cell receptor-antigen plus HLA class II association or whether T4-HLA-DR may transduce a signal towards B lymphocytes that is required in B cell activation remains unknown.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2944748

Citation

Fischer, A, et al. "Possible T4-HLA Class II Interaction as an Essential Event in Antigen-specific Helper T Lymphocyte-dependent B Cell Activation." European Journal of Immunology, vol. 16, no. 9, 1986, pp. 1111-6.
Fischer A, Sterkers G, Charron D, et al. Possible T4-HLA class II interaction as an essential event in antigen-specific helper T lymphocyte-dependent B cell activation. Eur J Immunol. 1986;16(9):1111-6.
Fischer, A., Sterkers, G., Charron, D., & Durandy, A. (1986). Possible T4-HLA class II interaction as an essential event in antigen-specific helper T lymphocyte-dependent B cell activation. European Journal of Immunology, 16(9), 1111-6.
Fischer A, et al. Possible T4-HLA Class II Interaction as an Essential Event in Antigen-specific Helper T Lymphocyte-dependent B Cell Activation. Eur J Immunol. 1986;16(9):1111-6. PubMed PMID: 2944748.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Possible T4-HLA class II interaction as an essential event in antigen-specific helper T lymphocyte-dependent B cell activation. AU - Fischer,A, AU - Sterkers,G, AU - Charron,D, AU - Durandy,A, PY - 1986/9/1/pubmed PY - 1986/9/1/medline PY - 1986/9/1/entrez SP - 1111 EP - 6 JF - European journal of immunology JO - Eur J Immunol VL - 16 IS - 9 N2 - We have previously shown that the interaction between influenza virus-specific helper T lymphocytes and B lymphocytes is HLA class II restricted (Fischer, A. et al., Eur. J. Immunol. 1985. 15: 620). In the present study, we used a panel of antibodies specific for the T4 and HLA-DR molecules to investigate the role of both structures in T-B lymphocyte interaction. Several anti-T4 monoclonal antibodies were shown to block the in vitro antibody production to A/Bangkok influenza virus while they were unable to inhibit the A/Bangkok-induced proliferation of specific helper T cell clones. Some anti-HLA-DR monoclonal antibodies inhibited the antibody production to A/Bangkok, the target of the inhibition being either monocytes or B lymphocytes. The incubation of helper T cell clones with an infra-inhibitory concentration of anti-T4 antibody and of B lymphocytes with an infra-inhibitory concentration of anti-HLA-DR antibody resulted in a mutual enhancement of inhibition. Such synergistic interactions were not observed using combinations of anti-T4 and anti-B membrane structures such as p35 or LFA-1 or anti-HLA-DR and anti-T membrane structures such as T11 or LFA-1. First, these results indicate that the T4 molecule plays an essential role in T-B cell interaction even when it is not absolutely required for T cell proliferation. Second, they strongly suggest that the T4 molecule directly interacts with HLA-DR at the B cell surface. Whether such interaction is required to enhance the stability of T3/T cell receptor-antigen plus HLA class II association or whether T4-HLA-DR may transduce a signal towards B lymphocytes that is required in B cell activation remains unknown. SN - 0014-2980 UR - https://www.unboundmedicine.com/medline/citation/2944748/Possible_T4_HLA_class_II_interaction_as_an_essential_event_in_antigen_specific_helper_T_lymphocyte_dependent_B_cell_activation_ L2 - https://doi.org/10.1002/eji.1830160914 DB - PRIME DP - Unbound Medicine ER -