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Extraction, purification and antioxidation of a polysaccharide from Fritillaria unibracteata var. wabuensis.
Int J Biol Macromol. 2018 Jun; 112:1073-1083.IJ

Abstract

Rich polysaccharides were directly observed in the bulbs of Fritillaria unibracteata var. wabuensis (FUW) using the periodic acid-Schiff (PAS) method and microexamination. An acidic water-soluble heteropolysaccharide (FWPS1-1) was isolated from FUW through ethanol precipitation, decoloration, deproteinization, dialysis and separation using a DE-52 anion-exchange column and a Sepharose G-150 gel filtration column. FWPS1-1 (average molecular weight: ~7.44 kDa) has many branches and long side chains; holds the triple-helix conformation; was composed of mannose (Man), galacturonic acid (GalA), galactose (Gal), xylose (Xyl) and arabinose (Ara) with a molar ratio of 2.62:5.59:10.00:0.76:9.38; and features side chains that may be composed of Ara, Man, Gal and GalA, while the backbone may be composed of Xyl, Ara and Gal. In addition, the backbone of FWPS1-1 mainly consists of α-type glycosidic bonds. Bioactivity tests in vitro showed that the polysaccharide exhibited weak 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and low ferric reducing antioxidant power (FRAP) but high 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)l (ABTS) radical scavenging activity, good Fe(II)-chelating ability and remarkable DNA damage protective activity. FWPS1-1 was the first heteropolysaccharide purified from FUW and showed good antioxidant activity and DNA protective effect. The results confirmed that macromolecule is also bioactive ingredient that requires attention like the small-molecule active compounds in FUW.

Authors+Show Affiliations

Department of Production of Special Utilizated Plant, Agronomy College, Sichuan Agricultural University, No. 211, Huimin Rd, Wenjiang region, Chengdu 611130, Sichuan, PR China.Department of Production of Special Utilizated Plant, Agronomy College, Sichuan Agricultural University, No. 211, Huimin Rd, Wenjiang region, Chengdu 611130, Sichuan, PR China.Department of Production of Special Utilizated Plant, Agronomy College, Sichuan Agricultural University, No. 211, Huimin Rd, Wenjiang region, Chengdu 611130, Sichuan, PR China.Department of Production of Special Utilizated Plant, Agronomy College, Sichuan Agricultural University, No. 211, Huimin Rd, Wenjiang region, Chengdu 611130, Sichuan, PR China.Department of Production of Special Utilizated Plant, Agronomy College, Sichuan Agricultural University, No. 211, Huimin Rd, Wenjiang region, Chengdu 611130, Sichuan, PR China.Department of Production of Special Utilizated Plant, Agronomy College, Sichuan Agricultural University, No. 211, Huimin Rd, Wenjiang region, Chengdu 611130, Sichuan, PR China. Electronic address: ewuwei@sicau.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29447973

Citation

Pan, Feng, et al. "Extraction, Purification and Antioxidation of a Polysaccharide From Fritillaria Unibracteata Var. Wabuensis." International Journal of Biological Macromolecules, vol. 112, 2018, pp. 1073-1083.
Pan F, Su TJ, Liu Y, et al. Extraction, purification and antioxidation of a polysaccharide from Fritillaria unibracteata var. wabuensis. Int J Biol Macromol. 2018;112:1073-1083.
Pan, F., Su, T. J., Liu, Y., Hou, K., Chen, C., & Wu, W. (2018). Extraction, purification and antioxidation of a polysaccharide from Fritillaria unibracteata var. wabuensis. International Journal of Biological Macromolecules, 112, 1073-1083. https://doi.org/10.1016/j.ijbiomac.2018.02.070
Pan F, et al. Extraction, Purification and Antioxidation of a Polysaccharide From Fritillaria Unibracteata Var. Wabuensis. Int J Biol Macromol. 2018;112:1073-1083. PubMed PMID: 29447973.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extraction, purification and antioxidation of a polysaccharide from Fritillaria unibracteata var. wabuensis. AU - Pan,Feng, AU - Su,Tian-Jiao, AU - Liu,Yang, AU - Hou,Kai, AU - Chen,Chen, AU - Wu,Wei, Y1 - 2018/02/13/ PY - 2018/01/19/received PY - 2018/02/03/revised PY - 2018/02/11/accepted PY - 2018/2/16/pubmed PY - 2018/9/21/medline PY - 2018/2/16/entrez KW - Antioxidant activity KW - DNA damage protection KW - Fritillaria unibracteata var. wabuensis KW - Histochemical localization KW - Physicochemical property KW - Polysaccharide SP - 1073 EP - 1083 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 112 N2 - Rich polysaccharides were directly observed in the bulbs of Fritillaria unibracteata var. wabuensis (FUW) using the periodic acid-Schiff (PAS) method and microexamination. An acidic water-soluble heteropolysaccharide (FWPS1-1) was isolated from FUW through ethanol precipitation, decoloration, deproteinization, dialysis and separation using a DE-52 anion-exchange column and a Sepharose G-150 gel filtration column. FWPS1-1 (average molecular weight: ~7.44 kDa) has many branches and long side chains; holds the triple-helix conformation; was composed of mannose (Man), galacturonic acid (GalA), galactose (Gal), xylose (Xyl) and arabinose (Ara) with a molar ratio of 2.62:5.59:10.00:0.76:9.38; and features side chains that may be composed of Ara, Man, Gal and GalA, while the backbone may be composed of Xyl, Ara and Gal. In addition, the backbone of FWPS1-1 mainly consists of α-type glycosidic bonds. Bioactivity tests in vitro showed that the polysaccharide exhibited weak 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and low ferric reducing antioxidant power (FRAP) but high 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)l (ABTS) radical scavenging activity, good Fe(II)-chelating ability and remarkable DNA damage protective activity. FWPS1-1 was the first heteropolysaccharide purified from FUW and showed good antioxidant activity and DNA protective effect. The results confirmed that macromolecule is also bioactive ingredient that requires attention like the small-molecule active compounds in FUW. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/29447973/Extraction_purification_and_antioxidation_of_a_polysaccharide_from_Fritillaria_unibracteata_var__wabuensis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(18)30343-X DB - PRIME DP - Unbound Medicine ER -