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Altered Long Noncoding RNA and Messenger RNA Expression in Experimental Intracerebral Hemorrhage - a Preliminary Study.
Cell Physiol Biochem. 2018; 45(3):1284-1301.CP

Abstract

BACKGROUND/AIMS

Functional recovery in the chronic phase is a difficult problem in intracerebral hemorrhage (ICH) treatment. Long noncoding RNAs (lncRNAs) are demonstrated to be involved in central nervous system (CNS) disorders. However, the roles of lncRNAs in post-ICH injury and repair are poorly understood, especially those that may be attributed to long-term neurological deficit. The present study depicted the lncRNA and messenger RNA (mRNA) profile by microarray at late stage after an experimental ICH.

METHODS

LncRNA and mRNA microarray was used to first identify differentially expressed genes. Gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to determine bio-functions and signaling pathways, with which differentially expressed genes are most closely related. Quantitative real-time polymerase chain reaction (PCR) was used to validate the results of microarray. Finally, the lncRNA-mRNA co-expression network was constructed to find the interaction of genes.

RESULTS

A total of 625 differentially expressed lncRNAs and 826 expressed mRNAs were identified. Altered genes were enriched in mitochon-drial matrix, G-protein coupled receptor signaling pathway, and olfactory transduction, which may be associated with ICH-induced pathophysiologic changes in the long term. A co-expression network profile based on 5 validated differentially expressed lncRNAs and 205 interacted mRNAs was composed of 210 nodes and 298 connections.

CONCLUSION

Mitochondrial matrix, reduced G-protein coupled receptor activity, and impaired olfactory transduction may be involved in the sequelae following ICH. Further, these dysregulated lncRNAs and mRNAs may be the promising therapeutic targets to overcome obstacles in functional recovery following ICH.

Authors+Show Affiliations

Institute of Integrative Medicine, Xiangya Hospital, Central South University, Changsha, China.Institute of Integrative Medicine, Xiangya Hospital, Central South University, Changsha, China. Department of Gerontology, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University, Urumqi, China.Institute of Integrative Medicine, Xiangya Hospital, Central South University, Changsha, China.Institute of Neurology, Xiangya Hospital, Central South University, Changsha, China. Department of Neurology, Henan Province People's Hospital, Zhengzhou, China.Institute of Neurology, The First College of Clinical Medical Sciences, China Three Gorges University, Yichang, China.Institute of Integrative Medicine, Xiangya Hospital, Central South University, Changsha, China.Institute of Integrative Medicine, Xiangya Hospital, Central South University, Changsha, China.Institute of Integrative Medicine, Xiangya Hospital, Central South University, Changsha, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29448258

Citation

Hanjin, Cui, et al. "Altered Long Noncoding RNA and Messenger RNA Expression in Experimental Intracerebral Hemorrhage - a Preliminary Study." Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology, vol. 45, no. 3, 2018, pp. 1284-1301.
Hanjin C, Tao L, Pengfei L, et al. Altered Long Noncoding RNA and Messenger RNA Expression in Experimental Intracerebral Hemorrhage - a Preliminary Study. Cell Physiol Biochem. 2018;45(3):1284-1301.
Hanjin, C., Tao, L., Pengfei, L., Ali, Y., Huajun, Z., Jiekun, L., Yang, W., & Tao, T. (2018). Altered Long Noncoding RNA and Messenger RNA Expression in Experimental Intracerebral Hemorrhage - a Preliminary Study. Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology, 45(3), 1284-1301. https://doi.org/10.1159/000487464
Hanjin C, et al. Altered Long Noncoding RNA and Messenger RNA Expression in Experimental Intracerebral Hemorrhage - a Preliminary Study. Cell Physiol Biochem. 2018;45(3):1284-1301. PubMed PMID: 29448258.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Altered Long Noncoding RNA and Messenger RNA Expression in Experimental Intracerebral Hemorrhage - a Preliminary Study. AU - Hanjin,Cui, AU - Tao,Liu, AU - Pengfei,Li, AU - Ali,Yang, AU - Huajun,Zhou, AU - Jiekun,Luo, AU - Yang,Wang, AU - Tao,Tang, Y1 - 2018/02/09/ PY - 2017/08/07/received PY - 2017/12/14/accepted PY - 2018/2/16/pubmed PY - 2018/3/20/medline PY - 2018/2/16/entrez KW - Expression profile KW - Intracerebral hemorrhage KW - Long non-coding RNA (lncRNA) KW - Messenger RNA (mRNA) SP - 1284 EP - 1301 JF - Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology JO - Cell. Physiol. Biochem. VL - 45 IS - 3 N2 - BACKGROUND/AIMS: Functional recovery in the chronic phase is a difficult problem in intracerebral hemorrhage (ICH) treatment. Long noncoding RNAs (lncRNAs) are demonstrated to be involved in central nervous system (CNS) disorders. However, the roles of lncRNAs in post-ICH injury and repair are poorly understood, especially those that may be attributed to long-term neurological deficit. The present study depicted the lncRNA and messenger RNA (mRNA) profile by microarray at late stage after an experimental ICH. METHODS: LncRNA and mRNA microarray was used to first identify differentially expressed genes. Gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to determine bio-functions and signaling pathways, with which differentially expressed genes are most closely related. Quantitative real-time polymerase chain reaction (PCR) was used to validate the results of microarray. Finally, the lncRNA-mRNA co-expression network was constructed to find the interaction of genes. RESULTS: A total of 625 differentially expressed lncRNAs and 826 expressed mRNAs were identified. Altered genes were enriched in mitochon-drial matrix, G-protein coupled receptor signaling pathway, and olfactory transduction, which may be associated with ICH-induced pathophysiologic changes in the long term. A co-expression network profile based on 5 validated differentially expressed lncRNAs and 205 interacted mRNAs was composed of 210 nodes and 298 connections. CONCLUSION: Mitochondrial matrix, reduced G-protein coupled receptor activity, and impaired olfactory transduction may be involved in the sequelae following ICH. Further, these dysregulated lncRNAs and mRNAs may be the promising therapeutic targets to overcome obstacles in functional recovery following ICH. SN - 1421-9778 UR - https://www.unboundmedicine.com/medline/citation/29448258/Altered_Long_Noncoding_RNA_and_Messenger_RNA_Expression_in_Experimental_Intracerebral_Hemorrhage___a_Preliminary_Study_ L2 - https://www.karger.com?DOI=10.1159/000487464 DB - PRIME DP - Unbound Medicine ER -