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Experimental modelling of cardiac pressure overload hypertrophy: Modified technique for precise, reproducible, safe and easy aortic arch banding-debanding in mice.
Sci Rep 2018; 8(1):3167SR

Abstract

Pressure overload left ventricular hypertrophy is a known precursor of heart failure with ominous prognosis. The development of experimental models that reproduce this phenomenon is instrumental for the advancement in our understanding of its pathophysiology. The gold standard of these models is the controlled constriction of the mid aortic arch in mice according to Rockman's technique (RT). We developed a modified technique that allows individualized and fully controlled constriction of the aorta, improves efficiency and generates a reproducible stenosis that is technically easy to perform and release. An algorithm calculates, based on the echocardiographic arch diameter, the intended perimeter at the constriction, and a suture is prepared with two knots separated accordingly. The aorta is encircled twice with the suture and the loop is closed with a microclip under both knots. We performed controlled aortic constriction with Rockman's and the double loop-clip (DLC) techniques in mice. DLC proved superiority in efficiency (mortality and invalid experiments) and more homogeneity of the results (transcoarctational gradients, LV mass, cardiomyocyte hypertrophy, gene expression) than RT. DLC technique optimizes animal use and generates a consistent and customized aortic constriction with homogeneous LV pressure overload morphofunctional, structural, and molecular features.

Authors+Show Affiliations

Universidad de Cantabria, Facultad de Medicina, Santander, E-39011, Cantabria, Spain. Instituto de Investigación Valdecilla (IDIVAL), Cardenal Herrera Oria Av. s/n, Santander, E-39011, Cantabria, Spain.Hospital Universitario Marqués de Valdecilla, Avda. Valdecilla s/n, Santander, E-39008, Cantabria, Spain. Instituto de Investigación Valdecilla (IDIVAL), Cardenal Herrera Oria Av. s/n, Santander, E-39011, Cantabria, Spain.Hospital Universitario Marqués de Valdecilla, Avda. Valdecilla s/n, Santander, E-39008, Cantabria, Spain. Instituto de Investigación Valdecilla (IDIVAL), Cardenal Herrera Oria Av. s/n, Santander, E-39011, Cantabria, Spain.Hospital Universitario Marqués de Valdecilla, Avda. Valdecilla s/n, Santander, E-39008, Cantabria, Spain. Instituto de Investigación Valdecilla (IDIVAL), Cardenal Herrera Oria Av. s/n, Santander, E-39011, Cantabria, Spain.Hospital Universitario Marqués de Valdecilla, Avda. Valdecilla s/n, Santander, E-39008, Cantabria, Spain. Instituto de Investigación Valdecilla (IDIVAL), Cardenal Herrera Oria Av. s/n, Santander, E-39011, Cantabria, Spain.Universidad de Cantabria, Facultad de Medicina, Santander, E-39011, Cantabria, Spain. Instituto de Investigación Valdecilla (IDIVAL), Cardenal Herrera Oria Av. s/n, Santander, E-39011, Cantabria, Spain.Universidad de Cantabria, Facultad de Medicina, Santander, E-39011, Cantabria, Spain. hurlem@unican.es. Instituto de Investigación Valdecilla (IDIVAL), Cardenal Herrera Oria Av. s/n, Santander, E-39011, Cantabria, Spain. hurlem@unican.es.Hospital Universitario Marqués de Valdecilla, Avda. Valdecilla s/n, Santander, E-39008, Cantabria, Spain. jfnistal@gmail.com. Universidad de Cantabria, Facultad de Medicina, Santander, E-39011, Cantabria, Spain. jfnistal@gmail.com. Instituto de Investigación Valdecilla (IDIVAL), Cardenal Herrera Oria Av. s/n, Santander, E-39011, Cantabria, Spain. jfnistal@gmail.com. Centro de Investigación Biomédica en Red Cardiovascular (CIBERCV), Instituto de Salud Carlos III, Santander, Spain. jfnistal@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29453394

Citation

Merino, David, et al. "Experimental Modelling of Cardiac Pressure Overload Hypertrophy: Modified Technique for Precise, Reproducible, Safe and Easy Aortic Arch Banding-debanding in Mice." Scientific Reports, vol. 8, no. 1, 2018, p. 3167.
Merino D, Gil A, Gómez J, et al. Experimental modelling of cardiac pressure overload hypertrophy: Modified technique for precise, reproducible, safe and easy aortic arch banding-debanding in mice. Sci Rep. 2018;8(1):3167.
Merino, D., Gil, A., Gómez, J., Ruiz, L., Llano, M., García, R., ... Nistal, J. F. (2018). Experimental modelling of cardiac pressure overload hypertrophy: Modified technique for precise, reproducible, safe and easy aortic arch banding-debanding in mice. Scientific Reports, 8(1), p. 3167. doi:10.1038/s41598-018-21548-x.
Merino D, et al. Experimental Modelling of Cardiac Pressure Overload Hypertrophy: Modified Technique for Precise, Reproducible, Safe and Easy Aortic Arch Banding-debanding in Mice. Sci Rep. 2018 02 16;8(1):3167. PubMed PMID: 29453394.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Experimental modelling of cardiac pressure overload hypertrophy: Modified technique for precise, reproducible, safe and easy aortic arch banding-debanding in mice. AU - Merino,David, AU - Gil,Aritz, AU - Gómez,Jenny, AU - Ruiz,Luis, AU - Llano,Miguel, AU - García,Raquel, AU - Hurlé,María A, AU - Nistal,J Francisco, Y1 - 2018/02/16/ PY - 2017/11/20/received PY - 2018/02/06/accepted PY - 2018/2/18/entrez PY - 2018/2/18/pubmed PY - 2019/9/11/medline SP - 3167 EP - 3167 JF - Scientific reports JO - Sci Rep VL - 8 IS - 1 N2 - Pressure overload left ventricular hypertrophy is a known precursor of heart failure with ominous prognosis. The development of experimental models that reproduce this phenomenon is instrumental for the advancement in our understanding of its pathophysiology. The gold standard of these models is the controlled constriction of the mid aortic arch in mice according to Rockman's technique (RT). We developed a modified technique that allows individualized and fully controlled constriction of the aorta, improves efficiency and generates a reproducible stenosis that is technically easy to perform and release. An algorithm calculates, based on the echocardiographic arch diameter, the intended perimeter at the constriction, and a suture is prepared with two knots separated accordingly. The aorta is encircled twice with the suture and the loop is closed with a microclip under both knots. We performed controlled aortic constriction with Rockman's and the double loop-clip (DLC) techniques in mice. DLC proved superiority in efficiency (mortality and invalid experiments) and more homogeneity of the results (transcoarctational gradients, LV mass, cardiomyocyte hypertrophy, gene expression) than RT. DLC technique optimizes animal use and generates a consistent and customized aortic constriction with homogeneous LV pressure overload morphofunctional, structural, and molecular features. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/29453394/Experimental_modelling_of_cardiac_pressure_overload_hypertrophy:_Modified_technique_for_precise,_reproducible,_safe_and_easy_aortic_arch_banding-debanding_in_mice L2 - http://dx.doi.org/10.1038/s41598-018-21548-x DB - PRIME DP - Unbound Medicine ER -