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Dual nanoenzyme modified microelectrode based on carbon fiber coated with AuPd alloy nanoparticles decorated graphene quantum dots assembly for electrochemical detection in clinic cancer samples.
Biosens Bioelectron. 2018 Jun 01; 107:153-162.BB

Abstract

The development of high-efficient technologies for cancer biomarkers detection has attracted tremendous research effort for its great clinic significance. In this work, we designed a new type of flexible and robust nanohybrid microelectrode by modifying carbon fiber with dual nanoenzyme, i.e., AuPd alloy nanoparticles (AuPd-ANPs) decorated graphene quantum dots (GQDs) assembly, and explored its practical application in electrochemical sensing system for sensitive detection of cancer biomarker hydrogen peroxide (H2O2) in human breast cancer cells and tissue. For the preparation of dual nanoenzyme modified microelectrode, ionic liquid was used as the electrolyte for the effective electrodeposition of GQDs on carbon fiber substrate to form a close-packed assembly under a very negative potential, then the highly dense AuPd-ANPs were uniformly decorated on GQDs assembly by electrodeposition. In virtue of the structural merits and synergistic contribution of dual nanoenzyme in enhancing the electrocatalytic activity to H2O2, the resultant nanohybrid microelectrode exhibited good sensing performances for electrochemical detection of H2O2, including a high sensitivity of 371 μA cm-2 mM-1, a wide linear range from 1.0 μM to 18.44 mM, a low detection limit of 500 nM (a signal-to-noise ratio of 3:1), as well as good selectivity and biocompatibility, which could be used for real-time tracking H2O2 released from different types of human breast cells and in situ sensitive detection of H2O2 in clinical breast cancer tissue.

Authors+Show Affiliations

Key laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, PR China.Key laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, PR China.Key laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, PR China.Key laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, PR China.Key laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, PR China.Key laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, PR China.Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, PR China.Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, PR China.Hubei Key Laboratory of Plasma Chemistry and Advanced Materials, School of Materials Science and Engineering, Wuhan Institute of Technology, Wuhan 430073, PR China.Key laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, PR China. Electronic address: xiaofei@hust.edu.cn.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

29455025

Citation

Xu, Qi, et al. "Dual Nanoenzyme Modified Microelectrode Based On Carbon Fiber Coated With AuPd Alloy Nanoparticles Decorated Graphene Quantum Dots Assembly for Electrochemical Detection in Clinic Cancer Samples." Biosensors & Bioelectronics, vol. 107, 2018, pp. 153-162.
Xu Q, Yuan H, Dong X, et al. Dual nanoenzyme modified microelectrode based on carbon fiber coated with AuPd alloy nanoparticles decorated graphene quantum dots assembly for electrochemical detection in clinic cancer samples. Biosens Bioelectron. 2018;107:153-162.
Xu, Q., Yuan, H., Dong, X., Zhang, Y., Asif, M., Dong, Z., He, W., Ren, J., Sun, Y., & Xiao, F. (2018). Dual nanoenzyme modified microelectrode based on carbon fiber coated with AuPd alloy nanoparticles decorated graphene quantum dots assembly for electrochemical detection in clinic cancer samples. Biosensors & Bioelectronics, 107, 153-162. https://doi.org/10.1016/j.bios.2018.02.026
Xu Q, et al. Dual Nanoenzyme Modified Microelectrode Based On Carbon Fiber Coated With AuPd Alloy Nanoparticles Decorated Graphene Quantum Dots Assembly for Electrochemical Detection in Clinic Cancer Samples. Biosens Bioelectron. 2018 Jun 1;107:153-162. PubMed PMID: 29455025.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dual nanoenzyme modified microelectrode based on carbon fiber coated with AuPd alloy nanoparticles decorated graphene quantum dots assembly for electrochemical detection in clinic cancer samples. AU - Xu,Qi, AU - Yuan,Hao, AU - Dong,Xulin, AU - Zhang,Yan, AU - Asif,Muhammad, AU - Dong,Zehua, AU - He,Wenshan, AU - Ren,Jinghua, AU - Sun,Yimin, AU - Xiao,Fei, Y1 - 2018/02/09/ PY - 2017/12/16/received PY - 2018/01/27/revised PY - 2018/02/08/accepted PY - 2018/2/20/pubmed PY - 2018/9/11/medline PY - 2018/2/19/entrez KW - Alloy nanoparticles KW - Cancer biomarker detection KW - Carbon fiber microelectrode KW - Electrochemical sensor KW - Graphene quantum dots assembly SP - 153 EP - 162 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 107 N2 - The development of high-efficient technologies for cancer biomarkers detection has attracted tremendous research effort for its great clinic significance. In this work, we designed a new type of flexible and robust nanohybrid microelectrode by modifying carbon fiber with dual nanoenzyme, i.e., AuPd alloy nanoparticles (AuPd-ANPs) decorated graphene quantum dots (GQDs) assembly, and explored its practical application in electrochemical sensing system for sensitive detection of cancer biomarker hydrogen peroxide (H2O2) in human breast cancer cells and tissue. For the preparation of dual nanoenzyme modified microelectrode, ionic liquid was used as the electrolyte for the effective electrodeposition of GQDs on carbon fiber substrate to form a close-packed assembly under a very negative potential, then the highly dense AuPd-ANPs were uniformly decorated on GQDs assembly by electrodeposition. In virtue of the structural merits and synergistic contribution of dual nanoenzyme in enhancing the electrocatalytic activity to H2O2, the resultant nanohybrid microelectrode exhibited good sensing performances for electrochemical detection of H2O2, including a high sensitivity of 371 μA cm-2 mM-1, a wide linear range from 1.0 μM to 18.44 mM, a low detection limit of 500 nM (a signal-to-noise ratio of 3:1), as well as good selectivity and biocompatibility, which could be used for real-time tracking H2O2 released from different types of human breast cells and in situ sensitive detection of H2O2 in clinical breast cancer tissue. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/29455025/Dual_nanoenzyme_modified_microelectrode_based_on_carbon_fiber_coated_with_AuPd_alloy_nanoparticles_decorated_graphene_quantum_dots_assembly_for_electrochemical_detection_in_clinic_cancer_samples_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(18)30114-3 DB - PRIME DP - Unbound Medicine ER -