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Cloning and expression analysis of cDNAs encoding ADP-glucose pyrophosphorylase large and small subunits from hulless barley (Hordeum vulgare L. var. nudum).
Z Naturforsch C J Biosci. 2018 Apr 25; 73(5-6):191-197.ZN

Abstract

As an important plateau cereal crop, hulless barley is the principal food for the Tibetan people in China. ADP-glucose pyrophosphorylase (AGPase) is considered as the key enzyme for starch biosynthesis in plants. In this study, cDNAs encoding the small subunit (SSU I) and large subunit (LSU I) of AGPase were isolated from hulless barley. The results showed that SSU I and LSU I were 1438 and 1786 bp in length with a complete open reading frame (ORF) of 1419 and 1572 bp. The ORF-encoded polypeptides of 472 and 523 amino acids were having calculated molecular masses of 52.01 and 58.23 kDa, and the pI values were 5.59 and 6.30. In addition, phylogenetic analysis showed that SSU I and LSU I had the same phylogenetic trends with some species. Furthermore, expression levels in different growth periods and tissues of two hulless barley varieties were analyzed by quantitative reverse transcription-polymerase chain reaction. Gene expression levels of SSU I and LSU I were consistent with the total starch accumulation rate in endosperm. In conclusion, our data confirmed that SSU I and LSU I played an important role in hulless barley starch synthesis.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Li D
College of Agronomy, Sichuan Agricultural University, Chengdu 611130, P.R. China. College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, P.R. China.
Yang Z
College of Agronomy, Sichuan Agricultural University, Chengdu 611130, P.R. China.
Liu X
College of Agronomy, Sichuan Agricultural University, Chengdu 611130, P.R. China.
Song Z
College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, P.R. China.
Feng Z
College of Agronomy, Sichuan Agricultural University, Chengdu 611130, P.R. China.
He Y
College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, P.R. China.

MeSH

ChinaCloning, MolecularGlucose-1-Phosphate AdenylyltransferaseHordeumOpen Reading FramesPhylogenyPlant ProteinsSequence Alignment

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29455192

Citation

Li, Dongmei, et al. "Cloning and Expression Analysis of cDNAs Encoding ADP-glucose Pyrophosphorylase Large and Small Subunits From Hulless Barley (Hordeum Vulgare L. Var. Nudum)." Zeitschrift Fur Naturforschung. C, Journal of Biosciences, vol. 73, no. 5-6, 2018, pp. 191-197.
Li D, Yang Z, Liu X, et al. Cloning and expression analysis of cDNAs encoding ADP-glucose pyrophosphorylase large and small subunits from hulless barley (Hordeum vulgare L. var. nudum). Z Naturforsch C J Biosci. 2018;73(5-6):191-197.
Li, D., Yang, Z., Liu, X., Song, Z., Feng, Z., & He, Y. (2018). Cloning and expression analysis of cDNAs encoding ADP-glucose pyrophosphorylase large and small subunits from hulless barley (Hordeum vulgare L. var. nudum). Zeitschrift Fur Naturforschung. C, Journal of Biosciences, 73(5-6), 191-197. https://doi.org/10.1515/znc-2017-0154
Li D, et al. Cloning and Expression Analysis of cDNAs Encoding ADP-glucose Pyrophosphorylase Large and Small Subunits From Hulless Barley (Hordeum Vulgare L. Var. Nudum). Z Naturforsch C J Biosci. 2018 Apr 25;73(5-6):191-197. PubMed PMID: 29455192.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and expression analysis of cDNAs encoding ADP-glucose pyrophosphorylase large and small subunits from hulless barley (Hordeum vulgare L. var. nudum). AU - Li,Dongmei, AU - Yang,Zhimin, AU - Liu,Xinchun, AU - Song,Zhen, AU - Feng,Zongyun, AU - He,Yang, PY - 2017/08/28/received PY - 2018/01/20/accepted PY - 2018/2/20/pubmed PY - 2019/7/30/medline PY - 2018/2/19/entrez KW - ADP-glucose pyrophosphorylase KW - cloning KW - expression analysis KW - hulless barley SP - 191 EP - 197 JF - Zeitschrift fur Naturforschung. C, Journal of biosciences JO - Z Naturforsch C J Biosci VL - 73 IS - 5-6 N2 - As an important plateau cereal crop, hulless barley is the principal food for the Tibetan people in China. ADP-glucose pyrophosphorylase (AGPase) is considered as the key enzyme for starch biosynthesis in plants. In this study, cDNAs encoding the small subunit (SSU I) and large subunit (LSU I) of AGPase were isolated from hulless barley. The results showed that SSU I and LSU I were 1438 and 1786 bp in length with a complete open reading frame (ORF) of 1419 and 1572 bp. The ORF-encoded polypeptides of 472 and 523 amino acids were having calculated molecular masses of 52.01 and 58.23 kDa, and the pI values were 5.59 and 6.30. In addition, phylogenetic analysis showed that SSU I and LSU I had the same phylogenetic trends with some species. Furthermore, expression levels in different growth periods and tissues of two hulless barley varieties were analyzed by quantitative reverse transcription-polymerase chain reaction. Gene expression levels of SSU I and LSU I were consistent with the total starch accumulation rate in endosperm. In conclusion, our data confirmed that SSU I and LSU I played an important role in hulless barley starch synthesis. SN - 0939-5075 UR - https://www.unboundmedicine.com/medline/citation/29455192/Cloning_and_expression_analysis_of_cDNAs_encoding_ADP_glucose_pyrophosphorylase_large_and_small_subunits_from_hulless_barley__Hordeum_vulgare_L__var__nudum__ DB - PRIME DP - Unbound Medicine ER -