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Suppression of microRNA-144-3p attenuates oxygen-glucose deprivation/reoxygenation-induced neuronal injury by promoting Brg1/Nrf2/ARE signaling.
J Biochem Mol Toxicol. 2018 Apr; 32(4):e22044.JB

Abstract

Accumulating evidence has reported that microRNA-144-3p (miR-144-3p) is highly related to oxidative stress and apoptosis. However, little is known regarding its role in cerebral ischemia/reperfusion-induced neuronal injury. Herein, our results showed that miR-144-3p expression was significantly downregulated in neurons following oxygen-glucose deprivation and reoxygenation (OGD/R) treatment. Overexpression of miR-144-3p markedly reduced cell viability, promoted cell apoptosis, and increased oxidative stress in neurons with OGD/R treatment, whereas downregulation of miR-144-3p protected neurons against OGD/R-induced injury. Brahma-related gene 1 (Brg1) was identified as a potential target gene of miR-144-3p. Moreover, downregulation of miR-144-3p promoted the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) and increased antioxidant response element (ARE) activity. However, knockdown of Brg1 significantly abrogated the neuroprotective effects of miR-144-3p downregulation. Overall, our results suggest that miR-144-3p contributes to OGD/R-induced neuronal injury in vitro through negatively regulating Brg1/Nrf2/ARE signaling.

Authors+Show Affiliations

Department of Critical Care Medicine, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, 453100, China.Department of Intervention, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, 453100, China.Department of Critical Care Medicine, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, 453100, China.Department of Critical Care Medicine, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, 453100, China.Department of Critical Care Medicine, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, 453100, China.Department of General Surgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, 453100, China.Department of Imaging, Xinxiang Medical University, Xinxiang, Henan, 453003, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29457851

Citation

Li, Yanru, et al. "Suppression of microRNA-144-3p Attenuates Oxygen-glucose Deprivation/reoxygenation-induced Neuronal Injury By Promoting Brg1/Nrf2/ARE Signaling." Journal of Biochemical and Molecular Toxicology, vol. 32, no. 4, 2018, pp. e22044.
Li Y, Zhao Y, Cheng M, et al. Suppression of microRNA-144-3p attenuates oxygen-glucose deprivation/reoxygenation-induced neuronal injury by promoting Brg1/Nrf2/ARE signaling. J Biochem Mol Toxicol. 2018;32(4):e22044.
Li, Y., Zhao, Y., Cheng, M., Qiao, Y., Wang, Y., Xiong, W., & Yue, W. (2018). Suppression of microRNA-144-3p attenuates oxygen-glucose deprivation/reoxygenation-induced neuronal injury by promoting Brg1/Nrf2/ARE signaling. Journal of Biochemical and Molecular Toxicology, 32(4), e22044. https://doi.org/10.1002/jbt.22044
Li Y, et al. Suppression of microRNA-144-3p Attenuates Oxygen-glucose Deprivation/reoxygenation-induced Neuronal Injury By Promoting Brg1/Nrf2/ARE Signaling. J Biochem Mol Toxicol. 2018;32(4):e22044. PubMed PMID: 29457851.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Suppression of microRNA-144-3p attenuates oxygen-glucose deprivation/reoxygenation-induced neuronal injury by promoting Brg1/Nrf2/ARE signaling. AU - Li,Yanru, AU - Zhao,Yongli, AU - Cheng,Mingkun, AU - Qiao,Yingjie, AU - Wang,Yongtao, AU - Xiong,Wancheng, AU - Yue,Wei, Y1 - 2018/02/19/ PY - 2017/12/12/received PY - 2018/01/15/revised PY - 2018/01/20/accepted PY - 2018/2/20/pubmed PY - 2018/7/10/medline PY - 2018/2/20/entrez KW - Brg1 KW - Nrf2 KW - OGD/R KW - cerebral ischemia/reperfusion injury KW - miR-144-3p SP - e22044 EP - e22044 JF - Journal of biochemical and molecular toxicology JO - J. Biochem. Mol. Toxicol. VL - 32 IS - 4 N2 - Accumulating evidence has reported that microRNA-144-3p (miR-144-3p) is highly related to oxidative stress and apoptosis. However, little is known regarding its role in cerebral ischemia/reperfusion-induced neuronal injury. Herein, our results showed that miR-144-3p expression was significantly downregulated in neurons following oxygen-glucose deprivation and reoxygenation (OGD/R) treatment. Overexpression of miR-144-3p markedly reduced cell viability, promoted cell apoptosis, and increased oxidative stress in neurons with OGD/R treatment, whereas downregulation of miR-144-3p protected neurons against OGD/R-induced injury. Brahma-related gene 1 (Brg1) was identified as a potential target gene of miR-144-3p. Moreover, downregulation of miR-144-3p promoted the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) and increased antioxidant response element (ARE) activity. However, knockdown of Brg1 significantly abrogated the neuroprotective effects of miR-144-3p downregulation. Overall, our results suggest that miR-144-3p contributes to OGD/R-induced neuronal injury in vitro through negatively regulating Brg1/Nrf2/ARE signaling. SN - 1099-0461 UR - https://www.unboundmedicine.com/medline/citation/29457851/Suppression_of_microRNA_144_3p_attenuates_oxygen_glucose_deprivation/reoxygenation_induced_neuronal_injury_by_promoting_Brg1/Nrf2/ARE_signaling_ L2 - https://doi.org/10.1002/jbt.22044 DB - PRIME DP - Unbound Medicine ER -