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FGF 23, PTH and vitamin D status in end stage renal disease patients affected by VDR FokI and BsmI variants.
Clin Biochem. 2018 Apr; 54:42-50.CB

Abstract

OBJECTIVES

The aim of this study was to evaluate the association between two VDR SNPs FokI and BsmI and mineral status in ESRD patients.

DESIGN AND METHODS

Our case-control study included 100 patients with chronic renal failure in ESRD and 149 healthy subjects. We measured the serum Vitamin D levels and the serum intact PTH level by Electrochemiluminescence Technology (cobas E411 analyzer). We evaluated the serum FGF23 levels by indirect ELISA method. The genotyping of two VDR gene variants FokI and BsmI was carried out by PCR-RFLP technique.

RESULTS

In our study, the FokI TT genotype was associated with lower risk of ESRD development (OR = 0.176, Padj = 0.039). The difference in PTH and FGF23 levels between cases and controls was statistically significant. The expression of FokI CT genotype in subjects with diabetic nephropathy was associated with a negative correlation between VD and PTH levels (r = -0.620, P = 0.032) and a positive correlation between VD and FGF23 levels (r = 0.967, P = 0.012). A significant differences in VD levels between patients and controls was observed in the presence of FokI TT (P = 0.044) and CT (P = 0.036) genotypes. The expression of FGF23 serum level was significantly elevated in patients than in controls in the presence of the FokI CC and BsmI AG genotypes.

CONCLUSIONS

In conclusion, our study shows the existence of an association between VDR FokI, BsmI polymorphisms and mineral status in ESRD patients. The presence of VDR variants affect the protein expression of VD, phosphorus, FGF23 and PTH.

Authors+Show Affiliations

Immuno-Rheumatology Research Laboratory, Rheumatology Department, La Rabta Hospital, University of Tunis-El Manar, Tunis, Tunisia; Laboratory of Clinical Biochemistry and Hormonology, Pasteur Institute of Tunis, Tunis El Manar University, Tunisia. Electronic address: bouksilamouna@gmail.com.Department of Basic Sciences, Unit Research 12SP15 "Homeostasis and Cell Dysfunction", Medicine School of Tunis, Tunis El Manar University, Tunis 1007, Tunisia.Immuno-Rheumatology Research Laboratory, Rheumatology Department, La Rabta Hospital, University of Tunis-El Manar, Tunis, Tunisia; Laboratory of Clinical Biochemistry and Hormonology, Pasteur Institute of Tunis, Tunis El Manar University, Tunisia.Nephrology Department, La Rabta Hospital, Medicine School of Tunis, Tunis El Manar University, Tunis 1007, Tunisia.Immuno-Rheumatology Research Laboratory, Rheumatology Department, La Rabta Hospital, University of Tunis-El Manar, Tunis, Tunisia.Nephrology Department, La Rabta Hospital, Medicine School of Tunis, Tunis El Manar University, Tunis 1007, Tunisia.Department of Basic Sciences, Unit Research 12SP15 "Homeostasis and Cell Dysfunction", Medicine School of Tunis, Tunis El Manar University, Tunis 1007, Tunisia.Immuno-Rheumatology Research Laboratory, Rheumatology Department, La Rabta Hospital, University of Tunis-El Manar, Tunis, Tunisia; Laboratory of Clinical Biochemistry and Hormonology, Pasteur Institute of Tunis, Tunis El Manar University, Tunisia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29458003

Citation

Bouksila, Mouna, et al. "FGF 23, PTH and Vitamin D Status in End Stage Renal Disease Patients Affected By VDR FokI and BsmI Variants." Clinical Biochemistry, vol. 54, 2018, pp. 42-50.
Bouksila M, Kaabachi W, Mrad M, et al. FGF 23, PTH and vitamin D status in end stage renal disease patients affected by VDR FokI and BsmI variants. Clin Biochem. 2018;54:42-50.
Bouksila, M., Kaabachi, W., Mrad, M., Smaoui, W., El Kateb, E. C., Zouaghi, M. K., Hamzaoui, K., & Bahlous, A. (2018). FGF 23, PTH and vitamin D status in end stage renal disease patients affected by VDR FokI and BsmI variants. Clinical Biochemistry, 54, 42-50. https://doi.org/10.1016/j.clinbiochem.2018.02.009
Bouksila M, et al. FGF 23, PTH and Vitamin D Status in End Stage Renal Disease Patients Affected By VDR FokI and BsmI Variants. Clin Biochem. 2018;54:42-50. PubMed PMID: 29458003.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - FGF 23, PTH and vitamin D status in end stage renal disease patients affected by VDR FokI and BsmI variants. AU - Bouksila,Mouna, AU - Kaabachi,Wajih, AU - Mrad,Mehdi, AU - Smaoui,Wided, AU - El Kateb,Elhem Cheour, AU - Zouaghi,Mohammed Karim, AU - Hamzaoui,Kamel, AU - Bahlous,Afef, Y1 - 2018/02/16/ PY - 2017/11/22/received PY - 2018/02/13/revised PY - 2018/02/15/accepted PY - 2018/2/20/pubmed PY - 2018/7/13/medline PY - 2018/2/20/entrez SP - 42 EP - 50 JF - Clinical biochemistry JO - Clin Biochem VL - 54 N2 - OBJECTIVES: The aim of this study was to evaluate the association between two VDR SNPs FokI and BsmI and mineral status in ESRD patients. DESIGN AND METHODS: Our case-control study included 100 patients with chronic renal failure in ESRD and 149 healthy subjects. We measured the serum Vitamin D levels and the serum intact PTH level by Electrochemiluminescence Technology (cobas E411 analyzer). We evaluated the serum FGF23 levels by indirect ELISA method. The genotyping of two VDR gene variants FokI and BsmI was carried out by PCR-RFLP technique. RESULTS: In our study, the FokI TT genotype was associated with lower risk of ESRD development (OR = 0.176, Padj = 0.039). The difference in PTH and FGF23 levels between cases and controls was statistically significant. The expression of FokI CT genotype in subjects with diabetic nephropathy was associated with a negative correlation between VD and PTH levels (r = -0.620, P = 0.032) and a positive correlation between VD and FGF23 levels (r = 0.967, P = 0.012). A significant differences in VD levels between patients and controls was observed in the presence of FokI TT (P = 0.044) and CT (P = 0.036) genotypes. The expression of FGF23 serum level was significantly elevated in patients than in controls in the presence of the FokI CC and BsmI AG genotypes. CONCLUSIONS: In conclusion, our study shows the existence of an association between VDR FokI, BsmI polymorphisms and mineral status in ESRD patients. The presence of VDR variants affect the protein expression of VD, phosphorus, FGF23 and PTH. SN - 1873-2933 UR - https://www.unboundmedicine.com/medline/citation/29458003/FGF_23_PTH_and_vitamin_D_status_in_end_stage_renal_disease_patients_affected_by_VDR_FokI_and_BsmI_variants_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-9120(17)31160-8 DB - PRIME DP - Unbound Medicine ER -