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Sessein and isosessein with anti-inflammatory, antibacterial and antioxidant activity isolated from Salvia sessei Benth.
J Ethnopharmacol. 2018 May 10; 217:212-219.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Salvia sessei Benth, popularly known as "pipilolxochitl" or "sabanito", is a plant utilized in Mexico in traditional medicine for the treatment of erysipela. To date, only one report, to our knowledge, has been found in which a royleanone-type diterpene of the aerial parts of the species was isolated but, again to our knowledge, studies have not been conducted on the pharmacological activity of extracts and compounds isolated from this plant.

AIM OF THE STUDY

The objective of the study was to evaluate the anti-inflammatory, antibacterial and antioxidant activity of the organic extracts of the aerial parts of Salvia sessei Benth and sessein and isosessein isomers isolated from this.

MATERIALS AND METHODS

Anti-inflammatory activity was evaluated in a model of edema in mouse ear at 1 mg/ear of the isolated extracts and compounds (1 and 2), a dose-response curve was performed on these latter and one-half of the effective dose (ED50) was determined; antibacterial activity was determined through minimal inhibitory concentration (MIC) by microdilution at 100, 50, 25, and 12.5 µg/mL, and antioxidant capacity, by means of DPPH, ABTS, and FRAP assays where, for the first two assays noted, the inhibitory concentration 50% (IC50) was calculated for the extracts as well as for the compounds isolated.

RESULTS

The hexanic extracts (40.55 ± 0.5%), dichloromethanic (56.01 ± 1.1%) and methanolic (66.05 ± 0.3%), as well as isolated compounds 1 (79.85 ± 3.5%) and 2 (54.36 ± 1.7%), demonstrated anti-inflammatory activity; the methanolic extract presented the greatest percentage of inhibition, while isolated compounds 1 and 2 did not present a difference in their ED50; additionally, compound 1 exerted a similar effect to that of the drug-of-reference at the same dose (75.24 ± 2.4%). The antibacterial activity of the extracts and compounds was principally against Gram-positive bacteria: the hexanic extract presented activity against Staphylococcus hominis and the methanolic and dichloromethanic extracts, and compound 1 exhibited activity against Staphylococcus haemolyticus, S. hominis, E. faecalis, in addition to that Escherichia coli was sensitive to compound 1, while isomer 2 showed activity against Staphylococcus aureus, Staphylococcus haemolyticus, Staphylococcus epidermis and Streptococcus pyogenes bacteria related to erysipela. In the three assays, the extract demonstrating greatest antioxidant capacity was the methanolic extract, while that of the isolated compounds was compound 1.

CONCLUSIONS

The results show that the three extracts evaluated in the three models presented activity and the chromatographic separation of the dichloromethanic extract permitted the isolation of compounds 1 and 2 royleanone-type isomers, which also presented significant activities such as anti-inflammatory, antibacterial and antioxidant, thus validate the use of this species in traditional medicine.

Authors+Show Affiliations

Laboratorio de Química Productos Naturales y Farmacognosia, Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Av. Universidad No. 1001, Col. Chamilpa, C.P. 62209 Cuernavaca, Morelos, Mexico.Centro de Investigación Biomédica del Sur (CIBIS), IMSS Argentina 1, Col. Centro, C.P. 62790 Xochitepec, Morelos, Mexico.Centro de Investigación Biomédica del Sur (CIBIS), IMSS Argentina 1, Col. Centro, C.P. 62790 Xochitepec, Morelos, Mexico.Centro de Investigación Biomédica del Sur (CIBIS), IMSS Argentina 1, Col. Centro, C.P. 62790 Xochitepec, Morelos, Mexico.Laboratorio de Química Productos Naturales y Farmacognosia, Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Av. Universidad No. 1001, Col. Chamilpa, C.P. 62209 Cuernavaca, Morelos, Mexico. Electronic address: veronica_rodriguez@uaem.mx.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29458147

Citation

Gómez-Rivera, Abraham, et al. "Sessein and Isosessein With Anti-inflammatory, Antibacterial and Antioxidant Activity Isolated From Salvia Sessei Benth." Journal of Ethnopharmacology, vol. 217, 2018, pp. 212-219.
Gómez-Rivera A, González-Cortazar M, Herrera-Ruíz M, et al. Sessein and isosessein with anti-inflammatory, antibacterial and antioxidant activity isolated from Salvia sessei Benth. J Ethnopharmacol. 2018;217:212-219.
Gómez-Rivera, A., González-Cortazar, M., Herrera-Ruíz, M., Zamilpa, A., & Rodríguez-López, V. (2018). Sessein and isosessein with anti-inflammatory, antibacterial and antioxidant activity isolated from Salvia sessei Benth. Journal of Ethnopharmacology, 217, 212-219. https://doi.org/10.1016/j.jep.2018.02.012
Gómez-Rivera A, et al. Sessein and Isosessein With Anti-inflammatory, Antibacterial and Antioxidant Activity Isolated From Salvia Sessei Benth. J Ethnopharmacol. 2018 May 10;217:212-219. PubMed PMID: 29458147.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sessein and isosessein with anti-inflammatory, antibacterial and antioxidant activity isolated from Salvia sessei Benth. AU - Gómez-Rivera,Abraham, AU - González-Cortazar,Manasés, AU - Herrera-Ruíz,Maribel, AU - Zamilpa,Alejandro, AU - Rodríguez-López,Verónica, Y1 - 2018/02/16/ PY - 2017/11/01/received PY - 2018/01/29/revised PY - 2018/02/06/accepted PY - 2018/2/20/pubmed PY - 2018/9/8/medline PY - 2018/2/20/entrez KW - Anti-inflammatory KW - Antibacterial KW - Antioxidant KW - Diterpenoids KW - Isosessein KW - Salvia sessei Benth KW - Sessein SP - 212 EP - 219 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 217 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Salvia sessei Benth, popularly known as "pipilolxochitl" or "sabanito", is a plant utilized in Mexico in traditional medicine for the treatment of erysipela. To date, only one report, to our knowledge, has been found in which a royleanone-type diterpene of the aerial parts of the species was isolated but, again to our knowledge, studies have not been conducted on the pharmacological activity of extracts and compounds isolated from this plant. AIM OF THE STUDY: The objective of the study was to evaluate the anti-inflammatory, antibacterial and antioxidant activity of the organic extracts of the aerial parts of Salvia sessei Benth and sessein and isosessein isomers isolated from this. MATERIALS AND METHODS: Anti-inflammatory activity was evaluated in a model of edema in mouse ear at 1 mg/ear of the isolated extracts and compounds (1 and 2), a dose-response curve was performed on these latter and one-half of the effective dose (ED50) was determined; antibacterial activity was determined through minimal inhibitory concentration (MIC) by microdilution at 100, 50, 25, and 12.5 µg/mL, and antioxidant capacity, by means of DPPH, ABTS, and FRAP assays where, for the first two assays noted, the inhibitory concentration 50% (IC50) was calculated for the extracts as well as for the compounds isolated. RESULTS: The hexanic extracts (40.55 ± 0.5%), dichloromethanic (56.01 ± 1.1%) and methanolic (66.05 ± 0.3%), as well as isolated compounds 1 (79.85 ± 3.5%) and 2 (54.36 ± 1.7%), demonstrated anti-inflammatory activity; the methanolic extract presented the greatest percentage of inhibition, while isolated compounds 1 and 2 did not present a difference in their ED50; additionally, compound 1 exerted a similar effect to that of the drug-of-reference at the same dose (75.24 ± 2.4%). The antibacterial activity of the extracts and compounds was principally against Gram-positive bacteria: the hexanic extract presented activity against Staphylococcus hominis and the methanolic and dichloromethanic extracts, and compound 1 exhibited activity against Staphylococcus haemolyticus, S. hominis, E. faecalis, in addition to that Escherichia coli was sensitive to compound 1, while isomer 2 showed activity against Staphylococcus aureus, Staphylococcus haemolyticus, Staphylococcus epidermis and Streptococcus pyogenes bacteria related to erysipela. In the three assays, the extract demonstrating greatest antioxidant capacity was the methanolic extract, while that of the isolated compounds was compound 1. CONCLUSIONS: The results show that the three extracts evaluated in the three models presented activity and the chromatographic separation of the dichloromethanic extract permitted the isolation of compounds 1 and 2 royleanone-type isomers, which also presented significant activities such as anti-inflammatory, antibacterial and antioxidant, thus validate the use of this species in traditional medicine. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/29458147/Sessein_and_isosessein_with_anti_inflammatory_antibacterial_and_antioxidant_activity_isolated_from_Salvia_sessei_Benth_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(17)34010-2 DB - PRIME DP - Unbound Medicine ER -