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Carboxymethylated chitosan protects Schwann cells against hydrogen peroxide-induced apoptosis by inhibiting oxidative stress and mitochondria dependent pathway.
Eur J Pharmacol. 2018 Apr 15; 825:48-56.EJ

Abstract

Carboxymethylated chitosan (CMCS) has many beneficial effects, including anti-oxidant and anti-apoptotic actions. However, the mechanisms by which CMCS protect against oxidative stress induced damage to Schwann cells (SCs) remains unclear. The present study aimed to investigate the mechanism by which CMCS protects SCs against hydrogen peroxide (H2O2) induced damage. H2O2 was used to establish a model of oxidative stress injury in SCs to mimic the development of nerve injury in vitro. Different concentrations (50, 100 and 200 µg/ml) of CMCS were added to test whether CMCS was capable of protecting SCs from H2O2 induced damage. MTT, LDH release and Annexin V/FITC assays were then performed. Levels of reactive oxygen species were detected using a reactive oxygen species assay kit, the mitochondrial membrane potential (ΔΨm) of SCs was analyzed by rhodamine123 fluorescence staining, the synthesis of Bcl-2, Bax, cytochrome c and caspase-3 were analyzed by real-time PCR and Western blot analysis. The results showed that CMCS protected SCs from apoptosis, decreased LDH release and enhanced cell viability, also decreased reactive oxygen species levels and increased ΔΨm. Additional experiments demonstrated that CMCS could decrease protein expression of Bax, cytochrome c and caspase-3, while promote Bcl-2 protein expression induced by H2O2. Taken together, the finding of this study indicated that CMCS prevented H2O2-induced damage to SCs through the mitochondrial dependent pathway.

Authors+Show Affiliations

Department of Orthopaedics, Renmin Hospital of Wuhan University, Wuhan, China. Electronic address: hebin@whu.edu.cn.Department of Orthopaedics, Renmin Hospital of Wuhan University, Wuhan, China.Department of Orthopaedics, Renmin Hospital of Wuhan University, Wuhan, China.Department of Orthopaedics, Renmin Hospital of Wuhan University, Wuhan, China.Department of Orthopaedics, Renmin Hospital of Wuhan University, Wuhan, China.Department of Orthopaedics, Renmin Hospital of Wuhan University, Wuhan, China.Department of Orthopaedics, Renmin Hospital of Wuhan University, Wuhan, China.Department of Orthopaedics, Renmin Hospital of Wuhan University, Wuhan, China.Department of Orthopaedics, Renmin Hospital of Wuhan University, Wuhan, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29462593

Citation

He, Bin, et al. "Carboxymethylated Chitosan Protects Schwann Cells Against Hydrogen Peroxide-induced Apoptosis By Inhibiting Oxidative Stress and Mitochondria Dependent Pathway." European Journal of Pharmacology, vol. 825, 2018, pp. 48-56.
He B, Wu F, Fan L, et al. Carboxymethylated chitosan protects Schwann cells against hydrogen peroxide-induced apoptosis by inhibiting oxidative stress and mitochondria dependent pathway. Eur J Pharmacol. 2018;825:48-56.
He, B., Wu, F., Fan, L., Li, X. H., Liu, Y., Liu, Y. J., Ding, W. J., Deng, M., & Zhou, Y. (2018). Carboxymethylated chitosan protects Schwann cells against hydrogen peroxide-induced apoptosis by inhibiting oxidative stress and mitochondria dependent pathway. European Journal of Pharmacology, 825, 48-56. https://doi.org/10.1016/j.ejphar.2018.02.024
He B, et al. Carboxymethylated Chitosan Protects Schwann Cells Against Hydrogen Peroxide-induced Apoptosis By Inhibiting Oxidative Stress and Mitochondria Dependent Pathway. Eur J Pharmacol. 2018 Apr 15;825:48-56. PubMed PMID: 29462593.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Carboxymethylated chitosan protects Schwann cells against hydrogen peroxide-induced apoptosis by inhibiting oxidative stress and mitochondria dependent pathway. AU - He,Bin, AU - Wu,Fei, AU - Fan,Li, AU - Li,Xiao-Hai, AU - Liu,Yang, AU - Liu,Ya-Jing, AU - Ding,Wan-Jun, AU - Deng,Ming, AU - Zhou,Yan, Y1 - 2018/02/17/ PY - 2017/11/03/received PY - 2018/02/10/revised PY - 2018/02/16/accepted PY - 2018/2/21/pubmed PY - 2018/9/7/medline PY - 2018/2/21/entrez KW - Apoptosis KW - Carboxymethylated chitosan KW - Hydrogen peroxide KW - Schwann cells KW - Signaling pathway SP - 48 EP - 56 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 825 N2 - Carboxymethylated chitosan (CMCS) has many beneficial effects, including anti-oxidant and anti-apoptotic actions. However, the mechanisms by which CMCS protect against oxidative stress induced damage to Schwann cells (SCs) remains unclear. The present study aimed to investigate the mechanism by which CMCS protects SCs against hydrogen peroxide (H2O2) induced damage. H2O2 was used to establish a model of oxidative stress injury in SCs to mimic the development of nerve injury in vitro. Different concentrations (50, 100 and 200 µg/ml) of CMCS were added to test whether CMCS was capable of protecting SCs from H2O2 induced damage. MTT, LDH release and Annexin V/FITC assays were then performed. Levels of reactive oxygen species were detected using a reactive oxygen species assay kit, the mitochondrial membrane potential (ΔΨm) of SCs was analyzed by rhodamine123 fluorescence staining, the synthesis of Bcl-2, Bax, cytochrome c and caspase-3 were analyzed by real-time PCR and Western blot analysis. The results showed that CMCS protected SCs from apoptosis, decreased LDH release and enhanced cell viability, also decreased reactive oxygen species levels and increased ΔΨm. Additional experiments demonstrated that CMCS could decrease protein expression of Bax, cytochrome c and caspase-3, while promote Bcl-2 protein expression induced by H2O2. Taken together, the finding of this study indicated that CMCS prevented H2O2-induced damage to SCs through the mitochondrial dependent pathway. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/29462593/Carboxymethylated_chitosan_protects_Schwann_cells_against_hydrogen_peroxide_induced_apoptosis_by_inhibiting_oxidative_stress_and_mitochondria_dependent_pathway_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(18)30101-8 DB - PRIME DP - Unbound Medicine ER -