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Distinguishing Human Peripheral Blood NK Cells from CD56dimCD16dimCD69+CD103+ Resident Nasal Mucosal Lavage Fluid Cells.

Abstract

Natural killer (NK) cells are members of the innate lymphoid cells group 1 (ILC1s), which play a critical role in innate host defense against viruses and malignancies. While many studies have examined the role of circulating peripheral blood (PB) CD56+ NK cells, little is known about the resident CD56+ cell population. Therefore, matched CD56+ cells from nasal lavage fluid (NLF) and PB of smokers and non-smokers were compared phenotypically, via flow cytometry, and functionally, via NK-cell specific gene expression. NLF and PB CD56+ cells had similar expression of CD56, but differentially expressed tissue residency (CD69 and CD103) and cytotoxicity (CD16) markers. In addition, NLF CD56dim cells expressed lower levels of cytotoxicity-associated genes, perforin (PRF1) and granzyme B (GZMB), and increased levels of cytokines and cell signaling molecules, TRAIL, IFNGR2, and IL8, as compared to PB CD56dim cells. In smokers, ITGA2 was downregulated in NLF CD56dim cells, while markers of cytotoxic function were primarily downregulated in PB CD56dim NK cells. Overall, NLF CD56dim cells are a unique cell population that likely play a role in orchestrating innate immune responses in the nasal cavity, which is distinct from their role as a non-antigen-restricted cytotoxic CD56dim lymphocytes in the PB.

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  • Authors+Show Affiliations

    ,

    Curriculum in Toxicology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

    ,

    Center for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

    ,

    Curriculum in Toxicology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

    ,

    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

    Curriculum in Toxicology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. ilona_jaspers@med.unc.edu. Center for Environmental Medicine, Asthma, and Lung Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. ilona_jaspers@med.unc.edu. Department of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. ilona_jaspers@med.unc.edu.

    Source

    Scientific reports 8:1 2018 02 21 pg 3394

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't
    Research Support, N.I.H., Extramural

    Language

    eng

    PubMed ID

    29467466

    Citation

    Rebuli, Meghan E., et al. "Distinguishing Human Peripheral Blood NK Cells From CD56dimCD16dimCD69+CD103+ Resident Nasal Mucosal Lavage Fluid Cells." Scientific Reports, vol. 8, no. 1, 2018, p. 3394.
    Rebuli ME, Pawlak EA, Walsh D, et al. Distinguishing Human Peripheral Blood NK Cells from CD56dimCD16dimCD69+CD103+ Resident Nasal Mucosal Lavage Fluid Cells. Sci Rep. 2018;8(1):3394.
    Rebuli, M. E., Pawlak, E. A., Walsh, D., Martin, E. M., & Jaspers, I. (2018). Distinguishing Human Peripheral Blood NK Cells from CD56dimCD16dimCD69+CD103+ Resident Nasal Mucosal Lavage Fluid Cells. Scientific Reports, 8(1), p. 3394. doi:10.1038/s41598-018-21443-5.
    Rebuli ME, et al. Distinguishing Human Peripheral Blood NK Cells From CD56dimCD16dimCD69+CD103+ Resident Nasal Mucosal Lavage Fluid Cells. Sci Rep. 2018 02 21;8(1):3394. PubMed PMID: 29467466.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Distinguishing Human Peripheral Blood NK Cells from CD56dimCD16dimCD69+CD103+ Resident Nasal Mucosal Lavage Fluid Cells. AU - Rebuli,Meghan E, AU - Pawlak,Erica A, AU - Walsh,Dana, AU - Martin,Elizabeth M, AU - Jaspers,Ilona, Y1 - 2018/02/21/ PY - 2017/06/05/received PY - 2018/02/02/accepted PY - 2018/2/23/entrez PY - 2018/2/23/pubmed PY - 2018/2/23/medline SP - 3394 EP - 3394 JF - Scientific reports JO - Sci Rep VL - 8 IS - 1 N2 - Natural killer (NK) cells are members of the innate lymphoid cells group 1 (ILC1s), which play a critical role in innate host defense against viruses and malignancies. While many studies have examined the role of circulating peripheral blood (PB) CD56+ NK cells, little is known about the resident CD56+ cell population. Therefore, matched CD56+ cells from nasal lavage fluid (NLF) and PB of smokers and non-smokers were compared phenotypically, via flow cytometry, and functionally, via NK-cell specific gene expression. NLF and PB CD56+ cells had similar expression of CD56, but differentially expressed tissue residency (CD69 and CD103) and cytotoxicity (CD16) markers. In addition, NLF CD56dim cells expressed lower levels of cytotoxicity-associated genes, perforin (PRF1) and granzyme B (GZMB), and increased levels of cytokines and cell signaling molecules, TRAIL, IFNGR2, and IL8, as compared to PB CD56dim cells. In smokers, ITGA2 was downregulated in NLF CD56dim cells, while markers of cytotoxic function were primarily downregulated in PB CD56dim NK cells. Overall, NLF CD56dim cells are a unique cell population that likely play a role in orchestrating innate immune responses in the nasal cavity, which is distinct from their role as a non-antigen-restricted cytotoxic CD56dim lymphocytes in the PB. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/29467466/Distinguishing_Human_Peripheral_Blood_NK_Cells_from_CD56dimCD16dimCD69+CD103+_Resident_Nasal_Mucosal_Lavage_Fluid_Cells_ L2 - http://dx.doi.org/10.1038/s41598-018-21443-5 DB - PRIME DP - Unbound Medicine ER -