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Novel sources of resistance to Septoria nodorum blotch in the Vavilov wheat collection identified by genome-wide association studies.
Theor Appl Genet. 2018 Jun; 131(6):1223-1238.TA

Abstract

KEY MESSAGE

The fungus Parastagonospora nodorum causes Septoria nodorum blotch (SNB) of wheat. A genetically diverse wheat panel was used to dissect the complexity of SNB and identify novel sources of resistance. The fungus Parastagonospora nodorum is the causal agent of Septoria nodorum blotch (SNB) of wheat. The pathosystem is mediated by multiple fungal necrotrophic effector-host sensitivity gene interactions that include SnToxA-Tsn1, SnTox1-Snn1, and SnTox3-Snn3. A P. nodorum strain lacking SnToxA, SnTox1, and SnTox3 (toxa13) retained wild-type-like ability to infect some modern wheat cultivars, suggesting evidence of other effector-mediated susceptibility gene interactions or the lack of host resistance genes. To identify genomic regions harbouring such loci, we examined a panel of 295 historic wheat accessions from the N. I. Vavilov Institute of Plant Genetic Resources in Russia, which is comprised of genetically diverse landraces and breeding lines registered from 1920 to 1990. The wheat panel was subjected to effector bioassays, infection with P. nodorum wild type (SN15) and toxa13. In general, SN15 was more virulent than toxa13. Insensitivity to all three effectors contributed significantly to resistance against SN15, but not toxa13. Genome-wide association studies using phenotypes from SN15 infection detected quantitative trait loci (QTL) on chromosomes 1BS (Snn1), 2DS, 5AS, 5BS (Snn3), 3AL, 4AL, 4BS, and 7AS. For toxa13 infection, a QTL was detected on 5AS (similar to SN15), plus two additional QTL on 2DL and 7DL. Analysis of resistance phenotypes indicated that plant breeders may have inadvertently selected for effector insensitivity from 1940 onwards. We identify accessions that can be used to develop bi-parental mapping populations to characterise resistance-associated alleles for subsequent introgression into modern bread wheat to minimise the impact of SNB.

Authors+Show Affiliations

Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia.Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia.Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia.Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia.Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD, Australia.Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD, Australia.Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia.Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia. Kar-Chun.Tan@curtin.edu.au.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29470621

Citation

Phan, Huyen T T., et al. "Novel Sources of Resistance to Septoria Nodorum Blotch in the Vavilov Wheat Collection Identified By Genome-wide Association Studies." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 131, no. 6, 2018, pp. 1223-1238.
Phan HTT, Rybak K, Bertazzoni S, et al. Novel sources of resistance to Septoria nodorum blotch in the Vavilov wheat collection identified by genome-wide association studies. Theor Appl Genet. 2018;131(6):1223-1238.
Phan, H. T. T., Rybak, K., Bertazzoni, S., Furuki, E., Dinglasan, E., Hickey, L. T., Oliver, R. P., & Tan, K. C. (2018). Novel sources of resistance to Septoria nodorum blotch in the Vavilov wheat collection identified by genome-wide association studies. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 131(6), 1223-1238. https://doi.org/10.1007/s00122-018-3073-y
Phan HTT, et al. Novel Sources of Resistance to Septoria Nodorum Blotch in the Vavilov Wheat Collection Identified By Genome-wide Association Studies. Theor Appl Genet. 2018;131(6):1223-1238. PubMed PMID: 29470621.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel sources of resistance to Septoria nodorum blotch in the Vavilov wheat collection identified by genome-wide association studies. AU - Phan,Huyen T T, AU - Rybak,Kasia, AU - Bertazzoni,Stefania, AU - Furuki,Eiko, AU - Dinglasan,Eric, AU - Hickey,Lee T, AU - Oliver,Richard P, AU - Tan,Kar-Chun, Y1 - 2018/02/22/ PY - 2017/11/14/received PY - 2018/02/16/accepted PY - 2018/2/23/pubmed PY - 2018/5/17/medline PY - 2018/2/23/entrez SP - 1223 EP - 1238 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 131 IS - 6 N2 - KEY MESSAGE: The fungus Parastagonospora nodorum causes Septoria nodorum blotch (SNB) of wheat. A genetically diverse wheat panel was used to dissect the complexity of SNB and identify novel sources of resistance. The fungus Parastagonospora nodorum is the causal agent of Septoria nodorum blotch (SNB) of wheat. The pathosystem is mediated by multiple fungal necrotrophic effector-host sensitivity gene interactions that include SnToxA-Tsn1, SnTox1-Snn1, and SnTox3-Snn3. A P. nodorum strain lacking SnToxA, SnTox1, and SnTox3 (toxa13) retained wild-type-like ability to infect some modern wheat cultivars, suggesting evidence of other effector-mediated susceptibility gene interactions or the lack of host resistance genes. To identify genomic regions harbouring such loci, we examined a panel of 295 historic wheat accessions from the N. I. Vavilov Institute of Plant Genetic Resources in Russia, which is comprised of genetically diverse landraces and breeding lines registered from 1920 to 1990. The wheat panel was subjected to effector bioassays, infection with P. nodorum wild type (SN15) and toxa13. In general, SN15 was more virulent than toxa13. Insensitivity to all three effectors contributed significantly to resistance against SN15, but not toxa13. Genome-wide association studies using phenotypes from SN15 infection detected quantitative trait loci (QTL) on chromosomes 1BS (Snn1), 2DS, 5AS, 5BS (Snn3), 3AL, 4AL, 4BS, and 7AS. For toxa13 infection, a QTL was detected on 5AS (similar to SN15), plus two additional QTL on 2DL and 7DL. Analysis of resistance phenotypes indicated that plant breeders may have inadvertently selected for effector insensitivity from 1940 onwards. We identify accessions that can be used to develop bi-parental mapping populations to characterise resistance-associated alleles for subsequent introgression into modern bread wheat to minimise the impact of SNB. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/29470621/Novel_sources_of_resistance_to_Septoria_nodorum_blotch_in_the_Vavilov_wheat_collection_identified_by_genome_wide_association_studies_ L2 - https://dx.doi.org/10.1007/s00122-018-3073-y DB - PRIME DP - Unbound Medicine ER -