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Isolation and molecular characterization of Corynebacterium pseudotuberculosis from sheep and goats in Mexico.
Microb Pathog. 2018 Apr; 117:304-309.MP

Abstract

The pathogenic bacteria of Corynebacterium pseudotuberculosis caused a chronic contagious infectious disease of the caseous lymphadenitis or pseudotuberculosis. Globally, isolates obtained from different injuries that affect sheep and goats, have been identified by fully or partially gene sequencing. However, in Mexico there is no complete study to identify by molecular and phylogenetic techniques the circulating isolates as well as its virulence factors. Therefore, in the present study we reported the identification of 57 isolates of C. pseudotuberculosis by bacteriological tests and the amplification of 16S rRNA, rpoB and pld genes, as well as, genes involved in virulence and pathogenicity: Fag A, Fag B, Fag C, Fag D and hsp60. Phylogenetic analysis was performed based on the partial sequence of the rpoB gene. Genes involved in virulence and pathogenicity were identified in the 98.2% of the isolates. Regarding the phylogenetic analysis, were identified the species and subspecies to which they belong of all the tested isolates. The phenotypic and genotypic characterization will allow to establish preventive and prophylactic measures aimed to the creation of effective immunogens against Corynebacterium pseudotuberculosis.

Authors+Show Affiliations

Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Km 15.5 Carretera Toluca, Atlacomulco, Estado de México, C.P. 50200, Mexico.Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Km 15.5 Carretera Toluca, Atlacomulco, Estado de México, C.P. 50200, Mexico. Electronic address: rmontesdeocaj@uaemex.mx.Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Km 15.5 Carretera Toluca, Atlacomulco, Estado de México, C.P. 50200, Mexico.Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Km 15.5 Carretera Toluca, Atlacomulco, Estado de México, C.P. 50200, Mexico.Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Km 15.5 Carretera Toluca, Atlacomulco, Estado de México, C.P. 50200, Mexico.Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Km 15.5 Carretera Toluca, Atlacomulco, Estado de México, C.P. 50200, Mexico.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29474828

Citation

Guerrero, Jorge Antonio Varela, et al. "Isolation and Molecular Characterization of Corynebacterium Pseudotuberculosis From Sheep and Goats in Mexico." Microbial Pathogenesis, vol. 117, 2018, pp. 304-309.
Guerrero JAV, de Oca Jiménez RM, Acosta Dibarrat J, et al. Isolation and molecular characterization of Corynebacterium pseudotuberculosis from sheep and goats in Mexico. Microb Pathog. 2018;117:304-309.
Guerrero, J. A. V., de Oca Jiménez, R. M., Acosta Dibarrat, J., León, F. H., Morales-Erasto, V., & Salazar, H. G. M. (2018). Isolation and molecular characterization of Corynebacterium pseudotuberculosis from sheep and goats in Mexico. Microbial Pathogenesis, 117, 304-309. https://doi.org/10.1016/j.micpath.2018.02.031
Guerrero JAV, et al. Isolation and Molecular Characterization of Corynebacterium Pseudotuberculosis From Sheep and Goats in Mexico. Microb Pathog. 2018;117:304-309. PubMed PMID: 29474828.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and molecular characterization of Corynebacterium pseudotuberculosis from sheep and goats in Mexico. AU - Guerrero,Jorge Antonio Varela, AU - de Oca Jiménez,Roberto Montes, AU - Acosta Dibarrat,Jorge, AU - León,Fernando Hernández, AU - Morales-Erasto,Vladimir, AU - Salazar,Humberto Gustavo Monroy, Y1 - 2018/02/20/ PY - 2018/01/17/received PY - 2018/02/03/revised PY - 2018/02/15/accepted PY - 2018/2/24/pubmed PY - 2018/8/29/medline PY - 2018/2/24/entrez KW - Corynebacterium pseudotuberculosis KW - Goats KW - Phylogenetic analysis KW - Sheep SP - 304 EP - 309 JF - Microbial pathogenesis JO - Microb. Pathog. VL - 117 N2 - The pathogenic bacteria of Corynebacterium pseudotuberculosis caused a chronic contagious infectious disease of the caseous lymphadenitis or pseudotuberculosis. Globally, isolates obtained from different injuries that affect sheep and goats, have been identified by fully or partially gene sequencing. However, in Mexico there is no complete study to identify by molecular and phylogenetic techniques the circulating isolates as well as its virulence factors. Therefore, in the present study we reported the identification of 57 isolates of C. pseudotuberculosis by bacteriological tests and the amplification of 16S rRNA, rpoB and pld genes, as well as, genes involved in virulence and pathogenicity: Fag A, Fag B, Fag C, Fag D and hsp60. Phylogenetic analysis was performed based on the partial sequence of the rpoB gene. Genes involved in virulence and pathogenicity were identified in the 98.2% of the isolates. Regarding the phylogenetic analysis, were identified the species and subspecies to which they belong of all the tested isolates. The phenotypic and genotypic characterization will allow to establish preventive and prophylactic measures aimed to the creation of effective immunogens against Corynebacterium pseudotuberculosis. SN - 1096-1208 UR - https://www.unboundmedicine.com/medline/citation/29474828/Isolation_and_molecular_characterization_of_Corynebacterium_pseudotuberculosis_from_sheep_and_goats_in_Mexico_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0882-4010(18)30098-6 DB - PRIME DP - Unbound Medicine ER -