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Intermediate filament protein expression pattern and inflammatory response changes in kidneys of rats receiving doxorubicin chemotherapy and quercetin.
Toxicol Lett. 2018 May 15; 288:89-98.TL

Abstract

The aim of this study was to explore the potential effects of quercetin (QUR) on doxorubicin (DOX)-induced nephrotoxicity. Fifty male rats were assigned to five groups (10 rats each): a control group, a DOX-treated group (total dose, 15 mg/kg bw, intraperitoneally), a QUR-treated group (50 mg/kg bw/day, orally), a prophylaxis co-treated group, and a therapeutic co-treated group. Biochemical parameters and renal function were measured. Moreover, kidney tissues were homogenized for inflammatory marker evaluation and real-time qPCR analysis to determine the changes in intermediate filament protein mRNA levels (desmin, vimentin, connexin 43 and nestin). QUR exhibited a significant nephroprotective effect, particularly when it was administered prior to and simultaneously with DOX treatment (prophylaxis co-treated group). This role was biochemically demonstrated by the significant modulation of DOX-induced body weight loss, hypoproteinemia, and elevated serum creatinine and urea. Moreover, QUR attenuated the inflammatory response as shown by decreased renal nitric oxide, tumor necrosis factor-α production and myeloperoxidase activity elicited by DOX injection. These biochemical improvements were accompanied by a significant histopathological restoration of rat kidney tissue and successful down-regulation of the intermediate filament protein mRNA levels, indicating amelioration of DOX-induced podocyte injury. Taken together, these results conclusively demonstrated that QUR administration has a prophylactic effect on DOX-induced injury in the rat kidney.

Authors+Show Affiliations

Forensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, Zagazig University, Egypt. Electronic address: resamah@zu.edu.eg.Forensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, Zagazig University, Egypt.Animal Wealth Development Department, Faculty of Veterinary Medicine, Zagazig University, Egypt.Animal Wealth Development Department, Faculty of Veterinary Medicine, Zagazig University, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29474904

Citation

Khalil, Samah R., et al. "Intermediate Filament Protein Expression Pattern and Inflammatory Response Changes in Kidneys of Rats Receiving Doxorubicin Chemotherapy and Quercetin." Toxicology Letters, vol. 288, 2018, pp. 89-98.
Khalil SR, Mohammed AT, Abd El-Fattah AH, et al. Intermediate filament protein expression pattern and inflammatory response changes in kidneys of rats receiving doxorubicin chemotherapy and quercetin. Toxicol Lett. 2018;288:89-98.
Khalil, S. R., Mohammed, A. T., Abd El-Fattah, A. H., & Zaglool, A. W. (2018). Intermediate filament protein expression pattern and inflammatory response changes in kidneys of rats receiving doxorubicin chemotherapy and quercetin. Toxicology Letters, 288, 89-98. https://doi.org/10.1016/j.toxlet.2018.02.024
Khalil SR, et al. Intermediate Filament Protein Expression Pattern and Inflammatory Response Changes in Kidneys of Rats Receiving Doxorubicin Chemotherapy and Quercetin. Toxicol Lett. 2018 May 15;288:89-98. PubMed PMID: 29474904.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intermediate filament protein expression pattern and inflammatory response changes in kidneys of rats receiving doxorubicin chemotherapy and quercetin. AU - Khalil,Samah R, AU - Mohammed,Amany Tharwat, AU - Abd El-Fattah,Amir H, AU - Zaglool,Asmaa W, Y1 - 2018/02/21/ PY - 2017/12/27/received PY - 2018/02/09/revised PY - 2018/02/14/accepted PY - 2018/2/24/pubmed PY - 2018/3/29/medline PY - 2018/2/24/entrez KW - Doxorubicin KW - Intermediate filament proteins KW - Myeloperoxidase KW - Podocytes KW - Quercetin SP - 89 EP - 98 JF - Toxicology letters JO - Toxicol Lett VL - 288 N2 - The aim of this study was to explore the potential effects of quercetin (QUR) on doxorubicin (DOX)-induced nephrotoxicity. Fifty male rats were assigned to five groups (10 rats each): a control group, a DOX-treated group (total dose, 15 mg/kg bw, intraperitoneally), a QUR-treated group (50 mg/kg bw/day, orally), a prophylaxis co-treated group, and a therapeutic co-treated group. Biochemical parameters and renal function were measured. Moreover, kidney tissues were homogenized for inflammatory marker evaluation and real-time qPCR analysis to determine the changes in intermediate filament protein mRNA levels (desmin, vimentin, connexin 43 and nestin). QUR exhibited a significant nephroprotective effect, particularly when it was administered prior to and simultaneously with DOX treatment (prophylaxis co-treated group). This role was biochemically demonstrated by the significant modulation of DOX-induced body weight loss, hypoproteinemia, and elevated serum creatinine and urea. Moreover, QUR attenuated the inflammatory response as shown by decreased renal nitric oxide, tumor necrosis factor-α production and myeloperoxidase activity elicited by DOX injection. These biochemical improvements were accompanied by a significant histopathological restoration of rat kidney tissue and successful down-regulation of the intermediate filament protein mRNA levels, indicating amelioration of DOX-induced podocyte injury. Taken together, these results conclusively demonstrated that QUR administration has a prophylactic effect on DOX-induced injury in the rat kidney. SN - 1879-3169 UR - https://www.unboundmedicine.com/medline/citation/29474904/Intermediate_filament_protein_expression_pattern_and_inflammatory_response_changes_in_kidneys_of_rats_receiving_doxorubicin_chemotherapy_and_quercetin_ DB - PRIME DP - Unbound Medicine ER -