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Behavioral and mutagenic biomarkers in tadpoles exposed to different abamectin concentrations.
Environ Sci Pollut Res Int. 2018 May; 25(13):12932-12946.ES

Abstract

It is known that pesticides such as abamectin (ABA) present cytotoxic effects on target organisms; however, the effects from ABA on non-target organisms such as amphibians are poorly understood. The aim of the current study is to investigate whether the exposure of Lithobates catesbeianus tadpoles to different abamectin concentrations [12.5, 25, and 50% of the median lethal concentration (LC50)] leads to behavioral and morphological changes and/or generates possible cytotoxic effects. The aggregation test showed that tadpoles exposed to the highest ABA concentrations did not respond to the stimulus from non-familial and unrelated co-specific species. On the other hand, there was no difference in the total number of crossings in the central line of the herein adopted apparatus between groups; it suggests that ABA did not affect animal locomotion in the aforementioned test, although changes in the normal swimming pattern of tadpoles exposed to the pesticide were recorded in the swimming activity test. In addition, the herein exposed animals did not respond to the predatory stimulus in the antipredator response test; this result suggests defensive response deficit caused by the pesticide. With respect to their oral morphology, tadpoles exposed to ABA presented the lowest scores for mandibular pigmentation and structures, as well as for dentition condition. Finally, it was possible seeing that the exposure to ABA, even at the lowest concentration (12.5% of the LC50), resulted in nuclear changes in the erythrocytes of the animals; these changes became evident in the increased number of micronuclei and in other nuclear abnormalities. Thus, besides confirming the cytotoxic potential of ABA in amphibians, the current study corroborates the hypothesis that the exposure to the herein investigated pesticide leads to behavioral and morphological changes in tadpoles, fact that may negatively reflect on the survival, as well as on natural populations of these individuals.

Authors+Show Affiliations

Post-graduation Program in Conservation of Cerrado Natural Resources - Biological Research Laboratory, Goiano Federal Institute - Urutaí Campus, Urutaí, GO, Brazil.Post-graduation Program in Conservation of Cerrado Natural Resources - Biological Research Laboratory, Goiano Federal Institute - Urutaí Campus, Urutaí, GO, Brazil.Post-graduation Program in Conservation of Cerrado Natural Resources - Biological Research Laboratory, Goiano Federal Institute - Urutaí Campus, Urutaí, GO, Brazil.Post-graduation Program in Conservation of Cerrado Natural Resources - Biological Research Laboratory, Goiano Federal Institute - Urutaí Campus, Urutaí, GO, Brazil. Biologigal Sciences Department, Post-Graduation Program in Conservation of Cerrado Natural Resources, Goiano Federal Institute - Urutaí Campus, Urutaí, GO, Brazil.Post-graduation Program in Conservation of Cerrado Natural Resources - Biological Research Laboratory, Goiano Federal Institute - Urutaí Campus, Urutaí, GO, Brazil. guilhermeifgoiano@gmail.com. Biologigal Sciences Department, Post-Graduation Program in Conservation of Cerrado Natural Resources, Goiano Federal Institute - Urutaí Campus, Urutaí, GO, Brazil. guilhermeifgoiano@gmail.com. Laboratório de Pesquisas Biológicas, Instituto Federal Goiano - Campus Urutaí, Urutaí, GO, Brazil. guilhermeifgoiano@gmail.com. Rodovia Geraldo Silva Nascimento, 2,5 km, Zona Rural, Urutaí, GO, CEP: 75790-000, Brazil. guilhermeifgoiano@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29478167

Citation

do Amaral, Diogo Ferreira, et al. "Behavioral and Mutagenic Biomarkers in Tadpoles Exposed to Different Abamectin Concentrations." Environmental Science and Pollution Research International, vol. 25, no. 13, 2018, pp. 12932-12946.
do Amaral DF, Montalvão MF, de Oliveira Mendes B, et al. Behavioral and mutagenic biomarkers in tadpoles exposed to different abamectin concentrations. Environ Sci Pollut Res Int. 2018;25(13):12932-12946.
do Amaral, D. F., Montalvão, M. F., de Oliveira Mendes, B., da Silva Castro, A. L., & Malafaia, G. (2018). Behavioral and mutagenic biomarkers in tadpoles exposed to different abamectin concentrations. Environmental Science and Pollution Research International, 25(13), 12932-12946. https://doi.org/10.1007/s11356-018-1562-9
do Amaral DF, et al. Behavioral and Mutagenic Biomarkers in Tadpoles Exposed to Different Abamectin Concentrations. Environ Sci Pollut Res Int. 2018;25(13):12932-12946. PubMed PMID: 29478167.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Behavioral and mutagenic biomarkers in tadpoles exposed to different abamectin concentrations. AU - do Amaral,Diogo Ferreira, AU - Montalvão,Mateus Flores, AU - de Oliveira Mendes,Bruna, AU - da Silva Castro,André Luis, AU - Malafaia,Guilherme, Y1 - 2018/02/24/ PY - 2017/12/18/received PY - 2018/02/13/accepted PY - 2018/2/27/pubmed PY - 2018/12/14/medline PY - 2018/2/26/entrez KW - Agrochemicals KW - Amphibians KW - Environmental toxicology KW - Pesticide SP - 12932 EP - 12946 JF - Environmental science and pollution research international JO - Environ Sci Pollut Res Int VL - 25 IS - 13 N2 - It is known that pesticides such as abamectin (ABA) present cytotoxic effects on target organisms; however, the effects from ABA on non-target organisms such as amphibians are poorly understood. The aim of the current study is to investigate whether the exposure of Lithobates catesbeianus tadpoles to different abamectin concentrations [12.5, 25, and 50% of the median lethal concentration (LC50)] leads to behavioral and morphological changes and/or generates possible cytotoxic effects. The aggregation test showed that tadpoles exposed to the highest ABA concentrations did not respond to the stimulus from non-familial and unrelated co-specific species. On the other hand, there was no difference in the total number of crossings in the central line of the herein adopted apparatus between groups; it suggests that ABA did not affect animal locomotion in the aforementioned test, although changes in the normal swimming pattern of tadpoles exposed to the pesticide were recorded in the swimming activity test. In addition, the herein exposed animals did not respond to the predatory stimulus in the antipredator response test; this result suggests defensive response deficit caused by the pesticide. With respect to their oral morphology, tadpoles exposed to ABA presented the lowest scores for mandibular pigmentation and structures, as well as for dentition condition. Finally, it was possible seeing that the exposure to ABA, even at the lowest concentration (12.5% of the LC50), resulted in nuclear changes in the erythrocytes of the animals; these changes became evident in the increased number of micronuclei and in other nuclear abnormalities. Thus, besides confirming the cytotoxic potential of ABA in amphibians, the current study corroborates the hypothesis that the exposure to the herein investigated pesticide leads to behavioral and morphological changes in tadpoles, fact that may negatively reflect on the survival, as well as on natural populations of these individuals. SN - 1614-7499 UR - https://www.unboundmedicine.com/medline/citation/29478167/Behavioral_and_mutagenic_biomarkers_in_tadpoles_exposed_to_different_abamectin_concentrations_ L2 - https://dx.doi.org/10.1007/s11356-018-1562-9 DB - PRIME DP - Unbound Medicine ER -