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Proteomic analysis reveals the distinct energy and protein metabolism characteristics involved in myofiber type conversion and resistance of atrophy in the extensor digitorum longus muscle of hibernating Daurian ground squirrels.

Abstract

Previous hibernation studies demonstrated that such a natural model of skeletal muscle disuse causes limited muscle atrophy and a significant fast-to-slow fiber type shift. However, the underlying mechanism as defined in a large-scale analysis remains unclarified. Isobaric tags for relative and absolute quantification (iTRAQ) based quantitative analysis were used to examine proteomic changes in the fast extensor digitorum longus muscles (EDL) of Daurian ground squirrels (Spermophilus dauricus). Although the wet weights and fiber cross-sectional area of the EDL muscle showed no significant decrease, the percentage of slow type fiber was 61% greater (P < 0.01) in the hibernation group. Proteomics analysis identified 264 proteins that were significantly changed (ratio < 0.83 or >1.2-fold and P < 0.05) in the hibernation group, of which 23 proteins were categorized into energy production and conversion and translation and 22 proteins were categorized into ribosomal structure and biogenesis. Along with the validation by western blot, MAPKAP kinase 2, ATP5D, ACADSB, calcineurin, CSTB and EIF2S were up-regulated in the hibernation group, whereas PDK4, COX II and EIF3C were down-regulated in the hibernation group. MAPKAP kinase 2 and PDK4 were associated with glycolysis, COX II and ATP5D were associated with oxidative phosphorylation, ACADSB was associated with fatty acid metabolism, calcineurin and CSTB were associated with catabolism, and EIF2S and EIF3C were associated with anabolism. Moreover, the total proteolysis rate of EDL in the hibernation group was significantly inhibited compared with that in the pre-hibernation group. These distinct energy and protein metabolism characteristics may be involved in myofiber type conversion and resistance to atrophy in the EDL of hibernating Daurian ground squirrels.

Authors+Show Affiliations

Shaanxi Key Laboratory for Animal Conservation, Northwest University, Xi'an 710069, PR China; Key Laboratory of Resource Biology and Biotechnology in Western China, College of Life Sciences, Northwest University, Ministry of Education, Xi'an 710069, PR China.Laboratory for Bone Metabolism, Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, PR China.Shaanxi Key Laboratory for Animal Conservation, Northwest University, Xi'an 710069, PR China.Shaanxi Key Laboratory for Animal Conservation, Northwest University, Xi'an 710069, PR China.Shaanxi Key Laboratory for Animal Conservation, Northwest University, Xi'an 710069, PR China.Shaanxi Key Laboratory for Animal Conservation, Northwest University, Xi'an 710069, PR China.Shaanxi Key Laboratory for Animal Conservation, Northwest University, Xi'an 710069, PR China.Shaanxi Key Laboratory for Animal Conservation, Northwest University, Xi'an 710069, PR China; Key Laboratory of Resource Biology and Biotechnology in Western China, College of Life Sciences, Northwest University, Ministry of Education, Xi'an 710069, PR China.Shaanxi Key Laboratory for Animal Conservation, Northwest University, Xi'an 710069, PR China; Key Laboratory of Resource Biology and Biotechnology in Western China, College of Life Sciences, Northwest University, Ministry of Education, Xi'an 710069, PR China. Electronic address: gaoyunf@nwu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29482114

Citation

Chang, Hui, et al. "Proteomic Analysis Reveals the Distinct Energy and Protein Metabolism Characteristics Involved in Myofiber Type Conversion and Resistance of Atrophy in the Extensor Digitorum Longus Muscle of Hibernating Daurian Ground Squirrels." Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics, vol. 26, 2018, pp. 20-31.
Chang H, Jiang S, Ma X, et al. Proteomic analysis reveals the distinct energy and protein metabolism characteristics involved in myofiber type conversion and resistance of atrophy in the extensor digitorum longus muscle of hibernating Daurian ground squirrels. Comp Biochem Physiol Part D Genomics Proteomics. 2018;26:20-31.
Chang, H., Jiang, S., Ma, X., Peng, X., Zhang, J., Wang, Z., ... Gao, Y. (2018). Proteomic analysis reveals the distinct energy and protein metabolism characteristics involved in myofiber type conversion and resistance of atrophy in the extensor digitorum longus muscle of hibernating Daurian ground squirrels. Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics, 26, pp. 20-31. doi:10.1016/j.cbd.2018.02.002.
Chang H, et al. Proteomic Analysis Reveals the Distinct Energy and Protein Metabolism Characteristics Involved in Myofiber Type Conversion and Resistance of Atrophy in the Extensor Digitorum Longus Muscle of Hibernating Daurian Ground Squirrels. Comp Biochem Physiol Part D Genomics Proteomics. 2018;26:20-31. PubMed PMID: 29482114.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteomic analysis reveals the distinct energy and protein metabolism characteristics involved in myofiber type conversion and resistance of atrophy in the extensor digitorum longus muscle of hibernating Daurian ground squirrels. AU - Chang,Hui, AU - Jiang,Shanfeng, AU - Ma,Xiufeng, AU - Peng,Xin, AU - Zhang,Jie, AU - Wang,Zhe, AU - Xu,Shenhui, AU - Wang,Huiping, AU - Gao,Yunfang, Y1 - 2018/02/13/ PY - 2017/09/06/received PY - 2018/02/08/revised PY - 2018/02/11/accepted PY - 2018/2/27/pubmed PY - 2019/1/25/medline PY - 2018/2/27/entrez KW - Daurian ground squirrel KW - Energy metabolism KW - Fiber type transition KW - Hibernation KW - Protein metabolism KW - Proteomic SP - 20 EP - 31 JF - Comparative biochemistry and physiology. Part D, Genomics & proteomics JO - Comp. Biochem. Physiol. Part D Genomics Proteomics VL - 26 N2 - Previous hibernation studies demonstrated that such a natural model of skeletal muscle disuse causes limited muscle atrophy and a significant fast-to-slow fiber type shift. However, the underlying mechanism as defined in a large-scale analysis remains unclarified. Isobaric tags for relative and absolute quantification (iTRAQ) based quantitative analysis were used to examine proteomic changes in the fast extensor digitorum longus muscles (EDL) of Daurian ground squirrels (Spermophilus dauricus). Although the wet weights and fiber cross-sectional area of the EDL muscle showed no significant decrease, the percentage of slow type fiber was 61% greater (P < 0.01) in the hibernation group. Proteomics analysis identified 264 proteins that were significantly changed (ratio < 0.83 or >1.2-fold and P < 0.05) in the hibernation group, of which 23 proteins were categorized into energy production and conversion and translation and 22 proteins were categorized into ribosomal structure and biogenesis. Along with the validation by western blot, MAPKAP kinase 2, ATP5D, ACADSB, calcineurin, CSTB and EIF2S were up-regulated in the hibernation group, whereas PDK4, COX II and EIF3C were down-regulated in the hibernation group. MAPKAP kinase 2 and PDK4 were associated with glycolysis, COX II and ATP5D were associated with oxidative phosphorylation, ACADSB was associated with fatty acid metabolism, calcineurin and CSTB were associated with catabolism, and EIF2S and EIF3C were associated with anabolism. Moreover, the total proteolysis rate of EDL in the hibernation group was significantly inhibited compared with that in the pre-hibernation group. These distinct energy and protein metabolism characteristics may be involved in myofiber type conversion and resistance to atrophy in the EDL of hibernating Daurian ground squirrels. SN - 1878-0407 UR - https://www.unboundmedicine.com/medline/citation/29482114/Proteomic_analysis_reveals_the_distinct_energy_and_protein_metabolism_characteristics_involved_in_myofiber_type_conversion_and_resistance_of_atrophy_in_the_extensor_digitorum_longus_muscle_of_hibernating_Daurian_ground_squirrels_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1744-117X(18)30009-1 DB - PRIME DP - Unbound Medicine ER -