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β-Cyanoalanine Synthase Action in Root Hair Elongation is Exerted at Early Steps of the Root Hair Elongation Pathway and is Independent of Direct Cyanide Inactivation of NADPH Oxidase.
Plant Cell Physiol 2018; 59(5):1072-1083PC

Abstract

In Arabidopsis thaliana, cyanide is produced concomitantly with ethylene biosynthesis and is mainly detoxified by the β-cyanoalanine synthase CAS-C1. In roots, CAS-C1 activity is essential to maintain a low level of cyanide for proper root hair development. Root hair elongation relies on polarized cell expansion at the growing tip, and we have observed that CAS-C1 locates in mitochondria and accumulates in root hair tips during root hair elongation, as shown by observing the fluorescence in plants transformed with the translational construct ProC1:CASC1-GFP, containing the complete CAS-C1 gene fused to green fluorescent protein (GFP). Mutants in the SUPERCENTIPEDE (SCN1) gene, that regulate the NADPH oxidase gene ROOT HAIR DEFECTIVE 2 (RHD2)/AtrbohC, are affected at the very early steps of the development of root hair that do not elongate and do not show a preferential localization of the GFP accumulation in the tips of the root hair primordia. Root hairs of mutants in CAS-C1 or RHD2/AtrbohC, whose protein product catalyzes the generation of ROS and the Ca2+ gradient, start to grow out correctly, but they do not elongate. Genetic crosses between the cas-c1 mutant and scn1 or rhd2 mutants were performed, and the detailed phenotypic and molecular characterization of the double mutants demonstrates that scn1 mutation is epistatic to cas-c1 and cas-c1 is epistatic to rhd2 mutation, indicating that CAS-C1 acts in early steps of the root hair development process. Moreover, our results show that the role of CAS-C1 in root hair elongation is independent of H2O2 production and of a direct NADPH oxidase inhibition by cyanide.

Authors+Show Affiliations

Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas and Universidad de Sevilla, Avenida Américo Vespucio, 49, Sevilla 41092, Spain.Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas and Universidad de Sevilla, Avenida Américo Vespucio, 49, Sevilla 41092, Spain.Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas and Universidad de Sevilla, Avenida Américo Vespucio, 49, Sevilla 41092, Spain.Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas and Universidad de Sevilla, Avenida Américo Vespucio, 49, Sevilla 41092, Spain.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29490083

Citation

Arenas-Alfonseca, Lucía, et al. "SS-Cyanoalanine Synthase Action in Root Hair Elongation Is Exerted at Early Steps of the Root Hair Elongation Pathway and Is Independent of Direct Cyanide Inactivation of NADPH Oxidase." Plant & Cell Physiology, vol. 59, no. 5, 2018, pp. 1072-1083.
Arenas-Alfonseca L, Gotor C, Romero LC, et al. SS-Cyanoalanine Synthase Action in Root Hair Elongation is Exerted at Early Steps of the Root Hair Elongation Pathway and is Independent of Direct Cyanide Inactivation of NADPH Oxidase. Plant Cell Physiol. 2018;59(5):1072-1083.
Arenas-Alfonseca, L., Gotor, C., Romero, L. C., & García, I. (2018). SS-Cyanoalanine Synthase Action in Root Hair Elongation is Exerted at Early Steps of the Root Hair Elongation Pathway and is Independent of Direct Cyanide Inactivation of NADPH Oxidase. Plant & Cell Physiology, 59(5), pp. 1072-1083. doi:10.1093/pcp/pcy047.
Arenas-Alfonseca L, et al. SS-Cyanoalanine Synthase Action in Root Hair Elongation Is Exerted at Early Steps of the Root Hair Elongation Pathway and Is Independent of Direct Cyanide Inactivation of NADPH Oxidase. Plant Cell Physiol. 2018 May 1;59(5):1072-1083. PubMed PMID: 29490083.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - β-Cyanoalanine Synthase Action in Root Hair Elongation is Exerted at Early Steps of the Root Hair Elongation Pathway and is Independent of Direct Cyanide Inactivation of NADPH Oxidase. AU - Arenas-Alfonseca,Lucía, AU - Gotor,Cecilia, AU - Romero,Luis C, AU - García,Irene, PY - 2017/10/23/received PY - 2018/02/22/accepted PY - 2018/3/1/pubmed PY - 2018/9/27/medline PY - 2018/3/1/entrez SP - 1072 EP - 1083 JF - Plant & cell physiology JO - Plant Cell Physiol. VL - 59 IS - 5 N2 - In Arabidopsis thaliana, cyanide is produced concomitantly with ethylene biosynthesis and is mainly detoxified by the β-cyanoalanine synthase CAS-C1. In roots, CAS-C1 activity is essential to maintain a low level of cyanide for proper root hair development. Root hair elongation relies on polarized cell expansion at the growing tip, and we have observed that CAS-C1 locates in mitochondria and accumulates in root hair tips during root hair elongation, as shown by observing the fluorescence in plants transformed with the translational construct ProC1:CASC1-GFP, containing the complete CAS-C1 gene fused to green fluorescent protein (GFP). Mutants in the SUPERCENTIPEDE (SCN1) gene, that regulate the NADPH oxidase gene ROOT HAIR DEFECTIVE 2 (RHD2)/AtrbohC, are affected at the very early steps of the development of root hair that do not elongate and do not show a preferential localization of the GFP accumulation in the tips of the root hair primordia. Root hairs of mutants in CAS-C1 or RHD2/AtrbohC, whose protein product catalyzes the generation of ROS and the Ca2+ gradient, start to grow out correctly, but they do not elongate. Genetic crosses between the cas-c1 mutant and scn1 or rhd2 mutants were performed, and the detailed phenotypic and molecular characterization of the double mutants demonstrates that scn1 mutation is epistatic to cas-c1 and cas-c1 is epistatic to rhd2 mutation, indicating that CAS-C1 acts in early steps of the root hair development process. Moreover, our results show that the role of CAS-C1 in root hair elongation is independent of H2O2 production and of a direct NADPH oxidase inhibition by cyanide. SN - 1471-9053 UR - https://www.unboundmedicine.com/medline/citation/29490083/β_Cyanoalanine_Synthase_Action_in_Root_Hair_Elongation_is_Exerted_at_Early_Steps_of_the_Root_Hair_Elongation_Pathway_and_is_Independent_of_Direct_Cyanide_Inactivation_of_NADPH_Oxidase_ L2 - https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcy047 DB - PRIME DP - Unbound Medicine ER -