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Massively Parallel Sequencing of Forensic STRs Using the Ion Chef™ and the Ion S5™ XL Systems.
J Forensic Sci. 2018 Nov; 63(6):1692-1703.JF

Abstract

Next-generation sequencing (NGS) has been used to genotype forensic short tandem repeat (STR) markers for individual identification and kinship analysis. STR data from several NGS platforms have been published, but forensic application trials using the Ion S5™ XL system have not been reported. In this work, we report sensitivity, reproducibility, mixture, simulated degradation, and casework sample data on the Ion Chef™ and S5™ XL systems using an early access 25-plex panel. Sensitivity experiments showed that over 97% of the alleles were detectable with down to 62 pg input of genomic DNA. In mixture studies, alleles from minor contributors were correctly assigned at 1:9 and 9:1 ratios. NGS successfully gave 12 full genotype results from 13 challenging casework samples, compared with five full results using the CE platform. In conclusion, the Ion Chef™ and the Ion S5™ XL systems provided an alternative and promising approach for forensic STR genotyping.

Authors+Show Affiliations

National Engineering Laboratory for Forensic Science and MPS Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, 100038, China.National Engineering Laboratory for Forensic Science and MPS Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, 100038, China.School of Forensic Medicine, Shanxi Medical University, Taiyuan, 030001, China.National Engineering Laboratory for Forensic Science and MPS Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, 100038, China.National Engineering Laboratory for Forensic Science and MPS Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, 100038, China.National Engineering Laboratory for Forensic Science and MPS Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, 100038, China.National Engineering Laboratory for Forensic Science and MPS Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, 100038, China.National Engineering Laboratory for Forensic Science and MPS Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, 100038, China.National Engineering Laboratory for Forensic Science and MPS Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, 100038, China.National Engineering Laboratory for Forensic Science and MPS Key Laboratory of Forensic Genetics, Institute of Forensic Science, Ministry of Public Security, Beijing, 100038, China.

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

29494760

Citation

Wang, Le, et al. "Massively Parallel Sequencing of Forensic STRs Using the Ion Chef™ and the Ion S5™ XL Systems." Journal of Forensic Sciences, vol. 63, no. 6, 2018, pp. 1692-1703.
Wang L, Chen M, Wu B, et al. Massively Parallel Sequencing of Forensic STRs Using the Ion Chef™ and the Ion S5™ XL Systems. J Forensic Sci. 2018;63(6):1692-1703.
Wang, L., Chen, M., Wu, B., Liu, Y. C., Zhang, G. F., Jiang, L., Xu, X. L., Zhao, X. C., Ji, A. Q., & Ye, J. (2018). Massively Parallel Sequencing of Forensic STRs Using the Ion Chef™ and the Ion S5™ XL Systems. Journal of Forensic Sciences, 63(6), 1692-1703. https://doi.org/10.1111/1556-4029.13767
Wang L, et al. Massively Parallel Sequencing of Forensic STRs Using the Ion Chef™ and the Ion S5™ XL Systems. J Forensic Sci. 2018;63(6):1692-1703. PubMed PMID: 29494760.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Massively Parallel Sequencing of Forensic STRs Using the Ion Chef™ and the Ion S5™ XL Systems. AU - Wang,Le, AU - Chen,Man, AU - Wu,Bo, AU - Liu,Yi-Cheng, AU - Zhang,Guang-Feng, AU - Jiang,Li, AU - Xu,Xiu-Lan, AU - Zhao,Xing-Chun, AU - Ji,An-Quan, AU - Ye,Jian, Y1 - 2018/03/01/ PY - 2017/08/08/received PY - 2017/10/25/revised PY - 2018/01/11/revised PY - 2018/02/03/revised PY - 2018/02/05/accepted PY - 2018/3/2/pubmed PY - 2018/12/12/medline PY - 2018/3/2/entrez KW - Ion Chef™ KW - Ion S5™ XL KW - forensic genetics KW - forensic science KW - next-generation sequencing KW - short tandem repeat SP - 1692 EP - 1703 JF - Journal of forensic sciences JO - J Forensic Sci VL - 63 IS - 6 N2 - Next-generation sequencing (NGS) has been used to genotype forensic short tandem repeat (STR) markers for individual identification and kinship analysis. STR data from several NGS platforms have been published, but forensic application trials using the Ion S5™ XL system have not been reported. In this work, we report sensitivity, reproducibility, mixture, simulated degradation, and casework sample data on the Ion Chef™ and S5™ XL systems using an early access 25-plex panel. Sensitivity experiments showed that over 97% of the alleles were detectable with down to 62 pg input of genomic DNA. In mixture studies, alleles from minor contributors were correctly assigned at 1:9 and 9:1 ratios. NGS successfully gave 12 full genotype results from 13 challenging casework samples, compared with five full results using the CE platform. In conclusion, the Ion Chef™ and the Ion S5™ XL systems provided an alternative and promising approach for forensic STR genotyping. SN - 1556-4029 UR - https://www.unboundmedicine.com/medline/citation/29494760/Massively_Parallel_Sequencing_of_Forensic_STRs_Using_the_Ion_Chef™_and_the_Ion_S5™_XL_Systems_ L2 - https://doi.org/10.1111/1556-4029.13767 DB - PRIME DP - Unbound Medicine ER -