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Increases in inflammatory and CD14dim/CD16pos/CD45pos patrolling monocytes in sepsis: correlation with final outcome.
Crit Care. 2018 03 03; 22(1):56.CC

Abstract

BACKGROUND

Evidence on the changes in the absolute counts of monocyte subpopulations in sepsis is missing.

METHODS

Firstly, absolute counts of circulating CD14pos/HLA-DRpos/CD45pos monocytes were measured by flow cytometry in 70 patients with Gram-negative sepsis and in 10 healthy volunteers. In the second phase, immunophenotyping was performed and the absolute count of circulating inflammatory monocytes and of circulating CD14dim/CD16pos/CD45pos patrolling monocytes were measured in another 55 patients and 10 healthy volunteers. Measurements were repeated on days 3, 7, and 10. Results were correlated with survival after 28 days.

RESULTS

Greater numbers of CD14pos/HLA-DRpos/CD45pos monocytes were found on day 1 in survivors compared to nonsurvivors (p = 0.030). Receiver operating characteristic (ROC) analysis showed that a cutoff higher than 337 cells/mm3 on day 1 could discriminate between survivors and nonsurvivors with a positive predictive value (PPV) of 91.1%. Logistic regression including Sequential Organ Failure Assessment (SOFA) score and Acute Physiology and Chronic Health Evaluation (APACHE) II score showed that an absolute count greater than 337 cells/mm3 was independently associated with unfavorable outcome (odds ratio (OR) 0.19, p = 0.050). The absolute counts of inflammatory and of CD14dim/CD16pos/CD45pos monocytes were greater in patients than healthy controls during the entire 10 days of follow-up. The absolute counts on day 3 of CD14dim/CD16pos/CD45pos monocytes were greater in survivors than nonsurvivors (p = 0.027). ROC analysis revealed that the cutoff at 27 cells/mm3 could discriminate between survivors and nonsurvivors with PPV of 94.1%. Logistic regression including age, SOFA score, and APACHE II score showed that an absolute count greater than 27 cells/mm3 was independently associated with unfavorable outcome (OR 0.06, p = 0.033). Logistic regression analysis showed that intra-abdominal infection on day 1 was predictive of low CD14dim/ CD16pos/CD45pos count on day 3.

CONCLUSION

Circulating counts of inflammatory and patrolling monocytes are greatly increased in Gram-negative sepsis. Absolute counts of CD14pos/HLA-DRpos/CD45pos monocytes on day 1 and CD14dim/CD16pos/CD45pos monocytes on day 3 are independently associated with final outcome.

TRIAL REGISTRATION

ClinicalTrials.gov, NCT01223690 . Registered retrospectively on 18 October 2010.

Authors+Show Affiliations

4th Department of Internal Medicine, National and Kapodistrian University of Athens, Medical School, ATTIKON University hospital, 1 Rimini Street, 12462, Athens, Greece.4th Department of Internal Medicine, National and Kapodistrian University of Athens, Medical School, ATTIKON University hospital, 1 Rimini Street, 12462, Athens, Greece.2nd Department of Critical Care Medicine, National and Kapodistrian University of Athens, Medical School, Athens, Greece.2nd Department of Internal Medicine, Sismanogleion Athens Hospital, Athens, Greece.4th Department of Internal Medicine, National and Kapodistrian University of Athens, Medical School, ATTIKON University hospital, 1 Rimini Street, 12462, Athens, Greece. egiamarel@med.uoa.gr.4th Department of Internal Medicine, National and Kapodistrian University of Athens, Medical School, ATTIKON University hospital, 1 Rimini Street, 12462, Athens, Greece.

Pub Type(s)

Journal Article
Observational Study

Language

eng

PubMed ID

29499723

Citation

Gainaru, Gabriela, et al. "Increases in Inflammatory and CD14dim/CD16pos/CD45pos Patrolling Monocytes in Sepsis: Correlation With Final Outcome." Critical Care (London, England), vol. 22, no. 1, 2018, p. 56.
Gainaru G, Papadopoulos A, Tsangaris I, et al. Increases in inflammatory and CD14dim/CD16pos/CD45pos patrolling monocytes in sepsis: correlation with final outcome. Crit Care. 2018;22(1):56.
Gainaru, G., Papadopoulos, A., Tsangaris, I., Lada, M., Giamarellos-Bourboulis, E. J., & Pistiki, A. (2018). Increases in inflammatory and CD14dim/CD16pos/CD45pos patrolling monocytes in sepsis: correlation with final outcome. Critical Care (London, England), 22(1), 56. https://doi.org/10.1186/s13054-018-1977-1
Gainaru G, et al. Increases in Inflammatory and CD14dim/CD16pos/CD45pos Patrolling Monocytes in Sepsis: Correlation With Final Outcome. Crit Care. 2018 03 3;22(1):56. PubMed PMID: 29499723.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increases in inflammatory and CD14dim/CD16pos/CD45pos patrolling monocytes in sepsis: correlation with final outcome. AU - Gainaru,Gabriela, AU - Papadopoulos,Antonios, AU - Tsangaris,Iraklis, AU - Lada,Malvina, AU - Giamarellos-Bourboulis,Evangelos J, AU - Pistiki,Aikaterini, Y1 - 2018/03/03/ PY - 2017/06/23/received PY - 2018/02/05/accepted PY - 2018/3/4/entrez PY - 2018/3/4/pubmed PY - 2018/10/3/medline KW - Gram-negative KW - Inflammatory monocytes KW - Patrolling monocytes KW - Sepsis KW - Survival SP - 56 EP - 56 JF - Critical care (London, England) JO - Crit Care VL - 22 IS - 1 N2 - BACKGROUND: Evidence on the changes in the absolute counts of monocyte subpopulations in sepsis is missing. METHODS: Firstly, absolute counts of circulating CD14pos/HLA-DRpos/CD45pos monocytes were measured by flow cytometry in 70 patients with Gram-negative sepsis and in 10 healthy volunteers. In the second phase, immunophenotyping was performed and the absolute count of circulating inflammatory monocytes and of circulating CD14dim/CD16pos/CD45pos patrolling monocytes were measured in another 55 patients and 10 healthy volunteers. Measurements were repeated on days 3, 7, and 10. Results were correlated with survival after 28 days. RESULTS: Greater numbers of CD14pos/HLA-DRpos/CD45pos monocytes were found on day 1 in survivors compared to nonsurvivors (p = 0.030). Receiver operating characteristic (ROC) analysis showed that a cutoff higher than 337 cells/mm3 on day 1 could discriminate between survivors and nonsurvivors with a positive predictive value (PPV) of 91.1%. Logistic regression including Sequential Organ Failure Assessment (SOFA) score and Acute Physiology and Chronic Health Evaluation (APACHE) II score showed that an absolute count greater than 337 cells/mm3 was independently associated with unfavorable outcome (odds ratio (OR) 0.19, p = 0.050). The absolute counts of inflammatory and of CD14dim/CD16pos/CD45pos monocytes were greater in patients than healthy controls during the entire 10 days of follow-up. The absolute counts on day 3 of CD14dim/CD16pos/CD45pos monocytes were greater in survivors than nonsurvivors (p = 0.027). ROC analysis revealed that the cutoff at 27 cells/mm3 could discriminate between survivors and nonsurvivors with PPV of 94.1%. Logistic regression including age, SOFA score, and APACHE II score showed that an absolute count greater than 27 cells/mm3 was independently associated with unfavorable outcome (OR 0.06, p = 0.033). Logistic regression analysis showed that intra-abdominal infection on day 1 was predictive of low CD14dim/ CD16pos/CD45pos count on day 3. CONCLUSION: Circulating counts of inflammatory and patrolling monocytes are greatly increased in Gram-negative sepsis. Absolute counts of CD14pos/HLA-DRpos/CD45pos monocytes on day 1 and CD14dim/CD16pos/CD45pos monocytes on day 3 are independently associated with final outcome. TRIAL REGISTRATION: ClinicalTrials.gov, NCT01223690 . Registered retrospectively on 18 October 2010. SN - 1466-609X UR - https://www.unboundmedicine.com/medline/citation/29499723/Increases_in_inflammatory_and_CD14dim/CD16pos/CD45pos_patrolling_monocytes_in_sepsis:_correlation_with_final_outcome_ L2 - https://ccforum.biomedcentral.com/articles/10.1186/s13054-018-1977-1 DB - PRIME DP - Unbound Medicine ER -