Tags

Type your tag names separated by a space and hit enter

Novel electrochemical aptasensor for ultrasensitive detection of sulfadimidine based on covalently linked multi-walled carbon nanotubes and in situ synthesized gold nanoparticle composites.
Anal Bioanal Chem. 2018 May; 410(12):2901-2910.AB

Abstract

In the current study, a sensitive electrochemical sensing strategy based on aptamer (APT) for detection of sulfadimidine (SM2) was developed. A bare gold electrode (AuE) was first modified with 2-aminoethanethiol (2-AET) through self-assembly, used as linker for the subsequent immobilization of multi-walled carbon nanotubes and gold nanoparticle composites (MWCNTs/AuNPs). Then, the thiolated APT was assembled onto the electrode via sulfur-gold affinity. When SM2 existed, the APT combined with SM2 and formed a complex structure. The specific binding of SM2 and APT increased the impedance, leading to hard electron transfer between the electrode surface and the redox probe [Fe(CN)6]3-/4- and producing a significant reduction of the signal. The SM2 concentration could be reflected by the current difference of the peak currents before and after target binding. Under optimized conditions, the linear dynamic range is from 0.1 to 50 ng mL-1, with a detection limit of 0.055 ng mL-1. The sensor exhibited desirable selectivity against other sulfonamides and performs successfully when analyzing SM2 in pork samples. Graphical abstract A new electrochemical biosensor for ultrasensitive detection of sulfadimidine (SM2) by using a gold electrode modified with MWCNTs/AuNPs for signal amplification and aptamer (APT) for selectivity improvement.

Authors+Show Affiliations

School of Food Science and Technology, Henan University of Technology, Lianhua Road 100#, Zhengzhou, Henan, 450001, China. hebaoshan@126.com.School of Food Science and Technology, Henan University of Technology, Lianhua Road 100#, Zhengzhou, Henan, 450001, China.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

29500483

Citation

He, Baoshan, and Gengan Du. "Novel Electrochemical Aptasensor for Ultrasensitive Detection of Sulfadimidine Based On Covalently Linked Multi-walled Carbon Nanotubes and in Situ Synthesized Gold Nanoparticle Composites." Analytical and Bioanalytical Chemistry, vol. 410, no. 12, 2018, pp. 2901-2910.
He B, Du G. Novel electrochemical aptasensor for ultrasensitive detection of sulfadimidine based on covalently linked multi-walled carbon nanotubes and in situ synthesized gold nanoparticle composites. Anal Bioanal Chem. 2018;410(12):2901-2910.
He, B., & Du, G. (2018). Novel electrochemical aptasensor for ultrasensitive detection of sulfadimidine based on covalently linked multi-walled carbon nanotubes and in situ synthesized gold nanoparticle composites. Analytical and Bioanalytical Chemistry, 410(12), 2901-2910. https://doi.org/10.1007/s00216-018-0970-5
He B, Du G. Novel Electrochemical Aptasensor for Ultrasensitive Detection of Sulfadimidine Based On Covalently Linked Multi-walled Carbon Nanotubes and in Situ Synthesized Gold Nanoparticle Composites. Anal Bioanal Chem. 2018;410(12):2901-2910. PubMed PMID: 29500483.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel electrochemical aptasensor for ultrasensitive detection of sulfadimidine based on covalently linked multi-walled carbon nanotubes and in situ synthesized gold nanoparticle composites. AU - He,Baoshan, AU - Du,Gengan, Y1 - 2018/03/03/ PY - 2017/11/21/received PY - 2018/02/17/accepted PY - 2018/01/01/revised PY - 2018/3/4/pubmed PY - 2018/5/11/medline PY - 2018/3/4/entrez KW - 2-Aminoethanethiol KW - Electrochemical aptasensor KW - Gold nanoparticles KW - Multi-walled carbon nanotubes KW - Sulfadimidine SP - 2901 EP - 2910 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 410 IS - 12 N2 - In the current study, a sensitive electrochemical sensing strategy based on aptamer (APT) for detection of sulfadimidine (SM2) was developed. A bare gold electrode (AuE) was first modified with 2-aminoethanethiol (2-AET) through self-assembly, used as linker for the subsequent immobilization of multi-walled carbon nanotubes and gold nanoparticle composites (MWCNTs/AuNPs). Then, the thiolated APT was assembled onto the electrode via sulfur-gold affinity. When SM2 existed, the APT combined with SM2 and formed a complex structure. The specific binding of SM2 and APT increased the impedance, leading to hard electron transfer between the electrode surface and the redox probe [Fe(CN)6]3-/4- and producing a significant reduction of the signal. The SM2 concentration could be reflected by the current difference of the peak currents before and after target binding. Under optimized conditions, the linear dynamic range is from 0.1 to 50 ng mL-1, with a detection limit of 0.055 ng mL-1. The sensor exhibited desirable selectivity against other sulfonamides and performs successfully when analyzing SM2 in pork samples. Graphical abstract A new electrochemical biosensor for ultrasensitive detection of sulfadimidine (SM2) by using a gold electrode modified with MWCNTs/AuNPs for signal amplification and aptamer (APT) for selectivity improvement. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/29500483/Novel_electrochemical_aptasensor_for_ultrasensitive_detection_of_sulfadimidine_based_on_covalently_linked_multi_walled_carbon_nanotubes_and_in_situ_synthesized_gold_nanoparticle_composites_ L2 - https://dx.doi.org/10.1007/s00216-018-0970-5 DB - PRIME DP - Unbound Medicine ER -