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Distribution and speciation of zinc in the gills of rainbow trout (Oncorhynchus mykiss) during acute waterborne zinc exposure: Interactions with cadmium or copper.
Comp Biochem Physiol C Toxicol Pharmacol. 2018 Apr; 206-207:23-31.CB

Abstract

We utilized micro X-ray fluorescence imaging (μ-XFI) and micro X-ray absorption near-edge spectroscopy (μ-XANES), which are both synchrotron-based techniques to investigate Zn distribution profile, its co-localization patterns with Ca, S, and Fe and speciation in the gills of rainbow trout (RBT). Fish (~100 g) were exposed to acutely toxic levels of waterborne Zn alone and in combination with waterborne Cd or Cu for 24 h (each at 1 × 96 h LC50). Gill sections were prepared and analyzed at the VESPERS beamline of the Canadian Light Source. The primary lamellae of the fish gill were found to be the primary area of Zn accumulation. These regions also correspond to the zones of mitochondria rich cells localization in fish gills, supporting the putative roles of these cells in metal uptake. Zn was also found to predominantly co-localize with Ca and S, but not with Fe, indicating the roles of Ca and S in intracellular Zn handling. Zn distribution in the gill was markedly reduced during co-exposure to Cd, but not to Cu, suggesting a competitive interaction between Zn and Cd for uptake. The speciation of Zn in the gill was dominated by Zn-phosphate, Zn-histidine and Zn-cysteine species; however, the interactions of Zn with Cd or Cu resulted in the loss of Zn-cysteine. Overall, our findings provide important novel insights into the interactions of Zn, Cd and Cu in the fish gill, which may ultimately help to explain the mechanisms underlying the acute toxicity of these metals in binary mixture to fish.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Saibu Y
Toxicology Centre, University of Saskatchewan, 44 Campus Drive, Saskatoon, SK, Canada. Electronic address: yusuf.saibu@usask.ca.
Jamwal A
Department of Biology, University of Saskatchewan, 112 Science Place, Saskatoon, SK, Canada.
Feng R
Canadian Light Source Incorporated, 44 Innovation Blvd, Saskatoon, SK, Canada.
Peak D
Department of Soil Science, University of Saskatchewan, 114 Science Place, Saskatoon, SK, Canada.
Niyogi S
Toxicology Centre, University of Saskatchewan, 44 Campus Drive, Saskatoon, SK, Canada; Department of Biology, University of Saskatchewan, 112 Science Place, Saskatoon, SK, Canada.

MeSH

AnimalsCadmiumCalciumCopperCysteineDrug InteractionsGillsHistidineMicroscopy, FluorescenceMitochondriaOncorhynchus mykissOrganometallic CompoundsPhosphatesSpectrometry, X-Ray EmissionSulfurTissue DistributionToxicity Tests, AcuteToxicokineticsWater Pollutants, ChemicalX-Ray Absorption SpectroscopyZincZinc Compounds

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29501824

Citation

Saibu, Yusuf, et al. "Distribution and Speciation of Zinc in the Gills of Rainbow Trout (Oncorhynchus Mykiss) During Acute Waterborne Zinc Exposure: Interactions With Cadmium or Copper." Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, vol. 206-207, 2018, pp. 23-31.
Saibu Y, Jamwal A, Feng R, et al. Distribution and speciation of zinc in the gills of rainbow trout (Oncorhynchus mykiss) during acute waterborne zinc exposure: Interactions with cadmium or copper. Comp Biochem Physiol C Toxicol Pharmacol. 2018;206-207:23-31.
Saibu, Y., Jamwal, A., Feng, R., Peak, D., & Niyogi, S. (2018). Distribution and speciation of zinc in the gills of rainbow trout (Oncorhynchus mykiss) during acute waterborne zinc exposure: Interactions with cadmium or copper. Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 206-207, 23-31. https://doi.org/10.1016/j.cbpc.2018.02.004
Saibu Y, et al. Distribution and Speciation of Zinc in the Gills of Rainbow Trout (Oncorhynchus Mykiss) During Acute Waterborne Zinc Exposure: Interactions With Cadmium or Copper. Comp Biochem Physiol C Toxicol Pharmacol. 2018;206-207:23-31. PubMed PMID: 29501824.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distribution and speciation of zinc in the gills of rainbow trout (Oncorhynchus mykiss) during acute waterborne zinc exposure: Interactions with cadmium or copper. AU - Saibu,Yusuf, AU - Jamwal,Ankur, AU - Feng,Renfei, AU - Peak,Derek, AU - Niyogi,Som, Y1 - 2018/03/01/ PY - 2018/01/12/received PY - 2018/02/21/revised PY - 2018/02/25/accepted PY - 2018/3/5/pubmed PY - 2018/7/26/medline PY - 2018/3/5/entrez KW - Distribution KW - Gill KW - Interactions KW - Metals KW - Rainbow trout KW - Speciation KW - X-ray spectroscopy SP - 23 EP - 31 JF - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP JO - Comp Biochem Physiol C Toxicol Pharmacol VL - 206-207 N2 - We utilized micro X-ray fluorescence imaging (μ-XFI) and micro X-ray absorption near-edge spectroscopy (μ-XANES), which are both synchrotron-based techniques to investigate Zn distribution profile, its co-localization patterns with Ca, S, and Fe and speciation in the gills of rainbow trout (RBT). Fish (~100 g) were exposed to acutely toxic levels of waterborne Zn alone and in combination with waterborne Cd or Cu for 24 h (each at 1 × 96 h LC50). Gill sections were prepared and analyzed at the VESPERS beamline of the Canadian Light Source. The primary lamellae of the fish gill were found to be the primary area of Zn accumulation. These regions also correspond to the zones of mitochondria rich cells localization in fish gills, supporting the putative roles of these cells in metal uptake. Zn was also found to predominantly co-localize with Ca and S, but not with Fe, indicating the roles of Ca and S in intracellular Zn handling. Zn distribution in the gill was markedly reduced during co-exposure to Cd, but not to Cu, suggesting a competitive interaction between Zn and Cd for uptake. The speciation of Zn in the gill was dominated by Zn-phosphate, Zn-histidine and Zn-cysteine species; however, the interactions of Zn with Cd or Cu resulted in the loss of Zn-cysteine. Overall, our findings provide important novel insights into the interactions of Zn, Cd and Cu in the fish gill, which may ultimately help to explain the mechanisms underlying the acute toxicity of these metals in binary mixture to fish. SN - 1532-0456 UR - https://www.unboundmedicine.com/medline/citation/29501824/Distribution_and_speciation_of_zinc_in_the_gills_of_rainbow_trout__Oncorhynchus_mykiss__during_acute_waterborne_zinc_exposure:_Interactions_with_cadmium_or_copper_ DB - PRIME DP - Unbound Medicine ER -
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