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Cedratvirus getuliensis replication cycle: an in-depth morphological analysis.
Sci Rep. 2018 03 05; 8(1):4000.SR

Abstract

The giant viruses are the largest and most complex viruses in the virosphere. In the last decade, new members have constantly been added to this group. Here, we provide an in-depth descriptive analysis of the replication cycle of Cedratvirus getuliensis, one of the largest viruses known to date. We tracked the virion entry, the early steps of virus factory and particles morphogenesis, and during this phase, we observed a complex and unique sequential organization of immature particle elements, including horseshoe and rectangular compartments, revealed by transverse and longitudinal sections, respectively, until the formation of the final ovoid-shaped striped virion. The genome and virion proteins are incorporated through a longitudinal opening in the immature virion, followed by the incorporation of the second cork and thickening of the capsid well. Moreover, many cell modifications occur during viral infection, including intense membrane trafficking important to viral morphogenesis and release, as evidenced by treatment using brefeldin A. Finally, we observed that Cedratvirus getuliensis particles are released after cellular lysis, although we obtained microscopic evidence that some particles are released by exocytosis. The present study provides new information on the unexplored steps in the life cycle of cedratviruses.

Authors+Show Affiliations

Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. Centro de Microscopia da Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. jonatas.abrahao@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29507337

Citation

Silva, Ludmila Karen Dos Santos, et al. "Cedratvirus Getuliensis Replication Cycle: an In-depth Morphological Analysis." Scientific Reports, vol. 8, no. 1, 2018, p. 4000.
Silva LKDS, Andrade ACDSP, Dornas FP, et al. Cedratvirus getuliensis replication cycle: an in-depth morphological analysis. Sci Rep. 2018;8(1):4000.
Silva, L. K. D. S., Andrade, A. C. D. S. P., Dornas, F. P., Rodrigues, R. A. L., Arantes, T., Kroon, E. G., Bonjardim, C. A., & Abrahão, J. S. (2018). Cedratvirus getuliensis replication cycle: an in-depth morphological analysis. Scientific Reports, 8(1), 4000. https://doi.org/10.1038/s41598-018-22398-3
Silva LKDS, et al. Cedratvirus Getuliensis Replication Cycle: an In-depth Morphological Analysis. Sci Rep. 2018 03 5;8(1):4000. PubMed PMID: 29507337.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cedratvirus getuliensis replication cycle: an in-depth morphological analysis. AU - Silva,Ludmila Karen Dos Santos, AU - Andrade,Ana Cláudia Dos Santos Pereira, AU - Dornas,Fábio Pio, AU - Rodrigues,Rodrigo Araújo Lima, AU - Arantes,Thalita, AU - Kroon,Erna Geessien, AU - Bonjardim,Cláudio Antônio, AU - Abrahão,Jônatas Santos, Y1 - 2018/03/05/ PY - 2018/01/09/received PY - 2018/02/22/accepted PY - 2018/3/7/entrez PY - 2018/3/7/pubmed PY - 2019/10/29/medline SP - 4000 EP - 4000 JF - Scientific reports JO - Sci Rep VL - 8 IS - 1 N2 - The giant viruses are the largest and most complex viruses in the virosphere. In the last decade, new members have constantly been added to this group. Here, we provide an in-depth descriptive analysis of the replication cycle of Cedratvirus getuliensis, one of the largest viruses known to date. We tracked the virion entry, the early steps of virus factory and particles morphogenesis, and during this phase, we observed a complex and unique sequential organization of immature particle elements, including horseshoe and rectangular compartments, revealed by transverse and longitudinal sections, respectively, until the formation of the final ovoid-shaped striped virion. The genome and virion proteins are incorporated through a longitudinal opening in the immature virion, followed by the incorporation of the second cork and thickening of the capsid well. Moreover, many cell modifications occur during viral infection, including intense membrane trafficking important to viral morphogenesis and release, as evidenced by treatment using brefeldin A. Finally, we observed that Cedratvirus getuliensis particles are released after cellular lysis, although we obtained microscopic evidence that some particles are released by exocytosis. The present study provides new information on the unexplored steps in the life cycle of cedratviruses. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/29507337/Cedratvirus_getuliensis_replication_cycle:_an_in_depth_morphological_analysis_ L2 - https://doi.org/10.1038/s41598-018-22398-3 DB - PRIME DP - Unbound Medicine ER -