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Distinct Profiles of CD163-Positive Macrophages in Idiopathic Interstitial Pneumonias.
J Immunol Res. 2018; 2018:1436236.JI

Abstract

Background

The types of cells most significantly linked to individual subtypes of idiopathic interstitial pneumonias (IIPs) remain unclear. Few studies have examined CD163+ macrophages in IIPs.

Objective

We retrospectively aimed to immunohistochemically characterize the CD163+ macrophages in IIPs.

Methods

Paraffin-embedded lung tissue samples were obtained from 47 patients with IIPs, including idiopathic pulmonary fibrosis (IPF), idiopathic nonspecific interstitial pneumonia (NSIP), and cryptogenic organizing pneumonia (COP), and 12 normal controls were immunohistochemically analyzed, using primary antibodies against CD68 and CD163 as indicators of pan and M2 macrophages, respectively.

Results

CD68+ macrophage density was significantly increased in the 3 subtypes of IIPs relative to that in the control group, although no difference was detected within the different IIPs. CD163+ macrophage density was significantly increased in NSIP and COP samples relative to that in IPF samples. The density ratio of CD163+ macrophages to CD68+ macrophages was significantly decreased in IPF/UIP samples relative to that in the others, while the densities in NSIP and COP were significantly higher than those in control cases.

Conclusion

CD163+ macrophages show distinct profiles among IIPs, and the standardized numerical density is decreased in IPF cases that have poor prognoses.

Authors+Show Affiliations

Department of Pulmonary Medicine, Allergy and Rheumatology, Iwate Medical University School of Medicine, Morioka, Japan.Department of Pathology, Tohoku University Graduate School of Medicine, Sendai, Japan.Department of Cancer Biology, Iwate Medical University, Shiwa, Japan.Division of Diagnostic Pathology, Itabashi Chuo Medical Center, Tokyo, Japan. Department of Analytic Human Pathology, Nippon Medical School, Tokyo, Japan.Department of Pathology, Tohoku University Graduate School of Medicine, Sendai, Japan.Department of Pathology, Iwate Medical University School of Medicine, Morioka, Japan.Department of Pulmonary Medicine, Allergy and Rheumatology, Iwate Medical University School of Medicine, Morioka, Japan.Department of Pulmonary Medicine, Allergy and Rheumatology, Iwate Medical University School of Medicine, Morioka, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29507864

Citation

Yamashita, Masahiro, et al. "Distinct Profiles of CD163-Positive Macrophages in Idiopathic Interstitial Pneumonias." Journal of Immunology Research, vol. 2018, 2018, p. 1436236.
Yamashita M, Saito R, Yasuhira S, et al. Distinct Profiles of CD163-Positive Macrophages in Idiopathic Interstitial Pneumonias. J Immunol Res. 2018;2018:1436236.
Yamashita, M., Saito, R., Yasuhira, S., Fukuda, Y., Sasamo, H., Sugai, T., Yamauchi, K., & Maemondo, M. (2018). Distinct Profiles of CD163-Positive Macrophages in Idiopathic Interstitial Pneumonias. Journal of Immunology Research, 2018, 1436236. https://doi.org/10.1155/2018/1436236
Yamashita M, et al. Distinct Profiles of CD163-Positive Macrophages in Idiopathic Interstitial Pneumonias. J Immunol Res. 2018;2018:1436236. PubMed PMID: 29507864.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distinct Profiles of CD163-Positive Macrophages in Idiopathic Interstitial Pneumonias. AU - Yamashita,Masahiro, AU - Saito,Ryoko, AU - Yasuhira,Shinji, AU - Fukuda,Yuh, AU - Sasamo,Hironobu, AU - Sugai,Tamotsu, AU - Yamauchi,Kohei, AU - Maemondo,Makoto, Y1 - 2018/02/04/ PY - 2017/10/26/received PY - 2017/12/14/accepted PY - 2018/3/7/entrez PY - 2018/3/7/pubmed PY - 2018/9/1/medline SP - 1436236 EP - 1436236 JF - Journal of immunology research JO - J Immunol Res VL - 2018 N2 - Background: The types of cells most significantly linked to individual subtypes of idiopathic interstitial pneumonias (IIPs) remain unclear. Few studies have examined CD163+ macrophages in IIPs. Objective: We retrospectively aimed to immunohistochemically characterize the CD163+ macrophages in IIPs. Methods: Paraffin-embedded lung tissue samples were obtained from 47 patients with IIPs, including idiopathic pulmonary fibrosis (IPF), idiopathic nonspecific interstitial pneumonia (NSIP), and cryptogenic organizing pneumonia (COP), and 12 normal controls were immunohistochemically analyzed, using primary antibodies against CD68 and CD163 as indicators of pan and M2 macrophages, respectively. Results: CD68+ macrophage density was significantly increased in the 3 subtypes of IIPs relative to that in the control group, although no difference was detected within the different IIPs. CD163+ macrophage density was significantly increased in NSIP and COP samples relative to that in IPF samples. The density ratio of CD163+ macrophages to CD68+ macrophages was significantly decreased in IPF/UIP samples relative to that in the others, while the densities in NSIP and COP were significantly higher than those in control cases. Conclusion: CD163+ macrophages show distinct profiles among IIPs, and the standardized numerical density is decreased in IPF cases that have poor prognoses. SN - 2314-7156 UR - https://www.unboundmedicine.com/medline/citation/29507864/Distinct_Profiles_of_CD163_Positive_Macrophages_in_Idiopathic_Interstitial_Pneumonias_ L2 - https://dx.doi.org/10.1155/2018/1436236 DB - PRIME DP - Unbound Medicine ER -