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Iron Phthalocyanine Decorated Nitrogen-Doped Graphene Biosensing Platform for Real-Time Detection of Nitric Oxide Released from Living Cells.
Anal Chem. 2018 04 03; 90(7):4438-4444.AC

Abstract

Nitric oxide (NO) is a transcellular messenger involved in many physiological and pathological processes, but the real-time detection of NO in biological systems is still challenging due to its rapid diffusion, low concentration, and short half-life. A novel electrochemical sensing platform based on iron phthalocyanine (FePc) functionalized nitrogen-doped graphene (N-G) nanocomposites was constructed to achieve in situ monitoring of NO released from living cells on the sensing layer. By taking advantage of the synergetic effect of N-G and FePc nanocomposites, the N-G/FePc sensor displays excellent electrocatalytic activity toward NO with a high sensitivity of 0.21 μA μM-1 cm-2 and a low detection limit of 180 nmol L-1. The following layer-by-layer assembly of poly-l-lysine (PLL) and Nafion further improved the capacity of resisting disturbance as well as the biocompatibility of the sensing interface. The flexible design of the ITO substrate based electrode provides a more controlled cellular biosensing system which could capture molecular signals immediately after NO released from human umbilical vein endothelial cells (HUVECs). The exhibited additional features of high sensitivity, rapid response, and ease of operation implies that the proposed N-G/FePc/Nafion/PLL ITO biosensor is a promising powerful platform in various complex biological systems.

Authors+Show Affiliations

Lab of Biosystem and Microanalysis, State Key Laboratory of Bioreactor Engineering , East China University of Science and Technology , Shanghai 200237 , China.Lab of Biosystem and Microanalysis, State Key Laboratory of Bioreactor Engineering , East China University of Science and Technology , Shanghai 200237 , China.Department of Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials and State Key Lab of Molecular Engineering of Polymers , Fudan University , Shanghai 200433 , China.Lab of Biosystem and Microanalysis, State Key Laboratory of Bioreactor Engineering , East China University of Science and Technology , Shanghai 200237 , China.Department of Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials and State Key Lab of Molecular Engineering of Polymers , Fudan University , Shanghai 200433 , China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29508999

Citation

Xu, Huiying, et al. "Iron Phthalocyanine Decorated Nitrogen-Doped Graphene Biosensing Platform for Real-Time Detection of Nitric Oxide Released From Living Cells." Analytical Chemistry, vol. 90, no. 7, 2018, pp. 4438-4444.
Xu H, Liao C, Liu Y, et al. Iron Phthalocyanine Decorated Nitrogen-Doped Graphene Biosensing Platform for Real-Time Detection of Nitric Oxide Released from Living Cells. Anal Chem. 2018;90(7):4438-4444.
Xu, H., Liao, C., Liu, Y., Ye, B. C., & Liu, B. (2018). Iron Phthalocyanine Decorated Nitrogen-Doped Graphene Biosensing Platform for Real-Time Detection of Nitric Oxide Released from Living Cells. Analytical Chemistry, 90(7), 4438-4444. https://doi.org/10.1021/acs.analchem.7b04419
Xu H, et al. Iron Phthalocyanine Decorated Nitrogen-Doped Graphene Biosensing Platform for Real-Time Detection of Nitric Oxide Released From Living Cells. Anal Chem. 2018 04 3;90(7):4438-4444. PubMed PMID: 29508999.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Iron Phthalocyanine Decorated Nitrogen-Doped Graphene Biosensing Platform for Real-Time Detection of Nitric Oxide Released from Living Cells. AU - Xu,Huiying, AU - Liao,Chong, AU - Liu,Yujie, AU - Ye,Bang-Ce, AU - Liu,Baohong, Y1 - 2018/03/13/ PY - 2018/3/7/pubmed PY - 2019/4/16/medline PY - 2018/3/7/entrez SP - 4438 EP - 4444 JF - Analytical chemistry JO - Anal. Chem. VL - 90 IS - 7 N2 - Nitric oxide (NO) is a transcellular messenger involved in many physiological and pathological processes, but the real-time detection of NO in biological systems is still challenging due to its rapid diffusion, low concentration, and short half-life. A novel electrochemical sensing platform based on iron phthalocyanine (FePc) functionalized nitrogen-doped graphene (N-G) nanocomposites was constructed to achieve in situ monitoring of NO released from living cells on the sensing layer. By taking advantage of the synergetic effect of N-G and FePc nanocomposites, the N-G/FePc sensor displays excellent electrocatalytic activity toward NO with a high sensitivity of 0.21 μA μM-1 cm-2 and a low detection limit of 180 nmol L-1. The following layer-by-layer assembly of poly-l-lysine (PLL) and Nafion further improved the capacity of resisting disturbance as well as the biocompatibility of the sensing interface. The flexible design of the ITO substrate based electrode provides a more controlled cellular biosensing system which could capture molecular signals immediately after NO released from human umbilical vein endothelial cells (HUVECs). The exhibited additional features of high sensitivity, rapid response, and ease of operation implies that the proposed N-G/FePc/Nafion/PLL ITO biosensor is a promising powerful platform in various complex biological systems. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/29508999/Iron_Phthalocyanine_Decorated_Nitrogen_Doped_Graphene_Biosensing_Platform_for_Real_Time_Detection_of_Nitric_Oxide_Released_from_Living_Cells_ L2 - https://doi.org/10.1021/acs.analchem.7b04419 DB - PRIME DP - Unbound Medicine ER -