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Next generation sequencing for gut microbiome characterization in rainbow trout (Oncorhynchus mykiss) fed animal by-product meals as an alternative to fishmeal protein sources.
PLoS One. 2018; 13(3):e0193652.Plos

Abstract

Animal by-product meals from the rendering industry could provide a sustainable and commercially viable alternative to fishmeal (FM) in aquaculture, as they are rich in most essential amino acids and contain important amounts of water-soluble proteins that improve feed digestibility and palatability. Among them, poultry by-product meal (PBM) have given encouraging results in rainbow trout (Oncorhynchus mykiss). However, the introduction of new ingredients in the diet needs to be carefully evaluated since diet is one of the main factors affecting the gut microbiota, which is a complex community that contributes to host metabolism, nutrition, growth, and disease resistance. Accordingly, we investigated the effects of partial replacement of dietary FM with a mix of animal by-product meals and plant proteins on intestinal microbiota composition of rainbow trout in relation to growth and feeding efficiency parameters. We used 1540 trout with an initial mean body weight of 94.6 ± 14.2 g. Fish were fed for 12 weeks with 7 different feed formulations. The growth data showed that trout fed on diets rich in animal by-product meals grew as well as fish fed on control diet, which was rich in FM (37.3%) and PBM-free. High-throughput 16S rRNA gene amplicon sequencing (MiSeq platform, Illumina) was utilised to study the gut microbial community profile. After discarding Cyanobacteria (class Chloroplast) and mitochondria reads a total of 2,701,274 of reads taxonomically classified, corresponding to a mean of 96,474 ± 68,056 reads per sample, were obtained. Five thousand three hundred ninety-nine operational taxonomic units (OTUs) were identified, which predominantly mapped to the phyla of Firmicutes, Proteobacteria, Bacteroidetes and Actinobacteria. The ratio between vegetable and animal proteins proved to play a central role in determining microbiome profiles and Firmicutes and Proteobacteria phyla were particularly discriminatory for diet type in trout. Plant ingredients favoured a higher Firmicutes:Proteobacteria ratio than animal proteins. Acceptable abundance of Firmicutes was guaranteed by including at least 25% of vegetable proteins in the diet regardless of animal protein source and percentage. In summary animal by-product meals, as replacements to FM, gave good results in terms of growth performances and did not induce significant changes in gut microbial richness, thus proving to be a suitable protein source for use in rainbow trout aqua feed.

Authors+Show Affiliations

Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy. Inter-University Centre for Research in Protein Biotechnologies "The Protein Factory"- Polytechnic University of Milan and University of Insubria, Varese, Italy.Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy.Fondazione Parco Tecnologico Padano, Lodi, Italy.VRM S.r.l. Naturalleva, Cologna Veneta, Verona, Italy.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29509788

Citation

Rimoldi, Simona, et al. "Next Generation Sequencing for Gut Microbiome Characterization in Rainbow Trout (Oncorhynchus Mykiss) Fed Animal By-product Meals as an Alternative to Fishmeal Protein Sources." PloS One, vol. 13, no. 3, 2018, pp. e0193652.
Rimoldi S, Terova G, Ascione C, et al. Next generation sequencing for gut microbiome characterization in rainbow trout (Oncorhynchus mykiss) fed animal by-product meals as an alternative to fishmeal protein sources. PLoS One. 2018;13(3):e0193652.
Rimoldi, S., Terova, G., Ascione, C., Giannico, R., & Brambilla, F. (2018). Next generation sequencing for gut microbiome characterization in rainbow trout (Oncorhynchus mykiss) fed animal by-product meals as an alternative to fishmeal protein sources. PloS One, 13(3), e0193652. https://doi.org/10.1371/journal.pone.0193652
Rimoldi S, et al. Next Generation Sequencing for Gut Microbiome Characterization in Rainbow Trout (Oncorhynchus Mykiss) Fed Animal By-product Meals as an Alternative to Fishmeal Protein Sources. PLoS One. 2018;13(3):e0193652. PubMed PMID: 29509788.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Next generation sequencing for gut microbiome characterization in rainbow trout (Oncorhynchus mykiss) fed animal by-product meals as an alternative to fishmeal protein sources. AU - Rimoldi,Simona, AU - Terova,Genciana, AU - Ascione,Chiara, AU - Giannico,Riccardo, AU - Brambilla,Fabio, Y1 - 2018/03/06/ PY - 2017/09/28/received PY - 2018/02/15/accepted PY - 2018/3/7/entrez PY - 2018/3/7/pubmed PY - 2018/6/28/medline SP - e0193652 EP - e0193652 JF - PloS one JO - PLoS One VL - 13 IS - 3 N2 - Animal by-product meals from the rendering industry could provide a sustainable and commercially viable alternative to fishmeal (FM) in aquaculture, as they are rich in most essential amino acids and contain important amounts of water-soluble proteins that improve feed digestibility and palatability. Among them, poultry by-product meal (PBM) have given encouraging results in rainbow trout (Oncorhynchus mykiss). However, the introduction of new ingredients in the diet needs to be carefully evaluated since diet is one of the main factors affecting the gut microbiota, which is a complex community that contributes to host metabolism, nutrition, growth, and disease resistance. Accordingly, we investigated the effects of partial replacement of dietary FM with a mix of animal by-product meals and plant proteins on intestinal microbiota composition of rainbow trout in relation to growth and feeding efficiency parameters. We used 1540 trout with an initial mean body weight of 94.6 ± 14.2 g. Fish were fed for 12 weeks with 7 different feed formulations. The growth data showed that trout fed on diets rich in animal by-product meals grew as well as fish fed on control diet, which was rich in FM (37.3%) and PBM-free. High-throughput 16S rRNA gene amplicon sequencing (MiSeq platform, Illumina) was utilised to study the gut microbial community profile. After discarding Cyanobacteria (class Chloroplast) and mitochondria reads a total of 2,701,274 of reads taxonomically classified, corresponding to a mean of 96,474 ± 68,056 reads per sample, were obtained. Five thousand three hundred ninety-nine operational taxonomic units (OTUs) were identified, which predominantly mapped to the phyla of Firmicutes, Proteobacteria, Bacteroidetes and Actinobacteria. The ratio between vegetable and animal proteins proved to play a central role in determining microbiome profiles and Firmicutes and Proteobacteria phyla were particularly discriminatory for diet type in trout. Plant ingredients favoured a higher Firmicutes:Proteobacteria ratio than animal proteins. Acceptable abundance of Firmicutes was guaranteed by including at least 25% of vegetable proteins in the diet regardless of animal protein source and percentage. In summary animal by-product meals, as replacements to FM, gave good results in terms of growth performances and did not induce significant changes in gut microbial richness, thus proving to be a suitable protein source for use in rainbow trout aqua feed. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/29509788/Next_generation_sequencing_for_gut_microbiome_characterization_in_rainbow_trout__Oncorhynchus_mykiss__fed_animal_by_product_meals_as_an_alternative_to_fishmeal_protein_sources_ DB - PRIME DP - Unbound Medicine ER -