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Biogenesis of silver nanoparticles using leaf extract of Indigofera hirsuta L. and their potential biomedical applications (3-in-1 system).

Abstract

The present study reports the biosynthesis of silver nanoparticles (IH-AgNPs) using aqueous leaf extract of Indigofera hisruta L. The biosynthesized IH-AgNPs were found to be FCC crystals, 5-10 nm in size, spherical in shape and stable. The biosynthesized IH-AgNPs showed dose-dependant cytotoxicity against prostate cancer (PC3) (IC50 = 68.5 μg/mL), colon cancer (COLO205) (IC50 = 85.2 μg/mL), and mouse melanoma (B16F10) (IC50 = 80.9 μg/mL). IH-AgNPs were found to be nontoxic towards normal CHO (Chinese hamster ovary) cells. The biosynthesized IH-AgNPs showed effective in vitro antioxidant activity against DPPH (IC50 = 63.43 μg/mL) and H2O2 (IC50 = 89.93 μg/mL) radicals. IH-AgNPs exhibited effective antibacterial activity against both Gram+ve and Gram-ve bacteria. MIC values of IH-AgNPs against S. aureus, B. subtilis, P. aeruginosa and E. coli were found to be 7.8 μg/mL, 3.9 μg/mL, 15.6 μg/mL and 15.6 μg/mL respectively. IH-AgNPs also showed inhibitory activity against fungal pathogens including C. albicans, C. nonalbicans and C. tropicalis. Considering the results together, we demonstrate that IH-AgNPs exhibits three different bioactivities (3-in-1 system) and they could be employed as future antimicrobial, antioxidant and anticancer agents/drug delivery vehicles in the field of biomedicine.

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  • Authors+Show Affiliations

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    a Department of Biotechnology , Sri Venkateswara University , Tirupati , India.

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    b Department of Biochemistry , Sri Venkateswara University , Tirupati , India.

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    a Department of Biotechnology , Sri Venkateswara University , Tirupati , India.

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    a Department of Biotechnology , Sri Venkateswara University , Tirupati , India.

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    a Department of Biotechnology , Sri Venkateswara University , Tirupati , India.

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    c Division of Biology , Indian Institute of Chemical Technology , Hyderabad , India.

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    d DST-PURSE Centre , Sri Venkateswara University , Tirupati , India.

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    a Department of Biotechnology , Sri Venkateswara University , Tirupati , India.

    e Department of Botany , Sri Venkateswara University , Tirupati , India.

    Source

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    29513113

    Citation

    TY - JOUR T1 - Biogenesis of silver nanoparticles using leaf extract of Indigofera hirsuta L. and their potential biomedical applications (3-in-1 system). AU - Netala,Vasudeva Reddy, AU - Bukke,Suman, AU - Domdi,Latha, AU - Soneya,S, AU - G Reddy,Sindhu, AU - Bethu,Murali Satyanarayana, AU - Kotakdi,Venkata Subbaiah, AU - Saritha,K V, AU - Tartte,Vijaya, Y1 - 2018/03/07/ PY - 2018/3/8/entrez PY - 2018/3/8/pubmed PY - 2018/3/8/medline KW - COLO205 and PC3 KW - Indigofera hirsuta KW - anticancer activity KW - antimicrobial activity KW - free radical scavenging activity KW - silver nanoparticles SP - 1 EP - 11 JF - Artificial cells, nanomedicine, and biotechnology JO - Artif Cells Nanomed Biotechnol N2 - The present study reports the biosynthesis of silver nanoparticles (IH-AgNPs) using aqueous leaf extract of Indigofera hisruta L. The biosynthesized IH-AgNPs were found to be FCC crystals, 5-10 nm in size, spherical in shape and stable. The biosynthesized IH-AgNPs showed dose-dependant cytotoxicity against prostate cancer (PC3) (IC50 = 68.5 μg/mL), colon cancer (COLO205) (IC50 = 85.2 μg/mL), and mouse melanoma (B16F10) (IC50 = 80.9 μg/mL). IH-AgNPs were found to be nontoxic towards normal CHO (Chinese hamster ovary) cells. The biosynthesized IH-AgNPs showed effective in vitro antioxidant activity against DPPH (IC50 = 63.43 μg/mL) and H2O2 (IC50 = 89.93 μg/mL) radicals. IH-AgNPs exhibited effective antibacterial activity against both Gram+ve and Gram-ve bacteria. MIC values of IH-AgNPs against S. aureus, B. subtilis, P. aeruginosa and E. coli were found to be 7.8 μg/mL, 3.9 μg/mL, 15.6 μg/mL and 15.6 μg/mL respectively. IH-AgNPs also showed inhibitory activity against fungal pathogens including C. albicans, C. nonalbicans and C. tropicalis. Considering the results together, we demonstrate that IH-AgNPs exhibits three different bioactivities (3-in-1 system) and they could be employed as future antimicrobial, antioxidant and anticancer agents/drug delivery vehicles in the field of biomedicine. SN - 2169-141X UR - https://www.unboundmedicine.com/medline/citation/29513113/Biogenesis_of_silver_nanoparticles_using_leaf_extract_of_Indigofera_hirsuta_L._and_their_potential_biomedical_applications_(3-in-1_system). L2 - http://www.tandfonline.com/doi/full/10.1080/21691401.2018.1446967 ER -