Tags

Type your tag names separated by a space and hit enter

HPLC METHOD FOR SEPARATING ENANTIOMERS OF IMIDAZOLE DERIVATIVES - ANTIFUNGAL COMPOUNDS.
Acta Pol Pharm 2017; 74(3):777-784AP

Abstract

The aim of this study was to test separation possibility of enantiomers of nine active substances belonging to imidazole derivatives: bifonazole, butoconazole, econazole, enilconazole, fenticonazole, isoconazole, miconazole, sertaconazole and tioconazole. The study was performed using HPLC method and the CHI- RALCEL OJ column (10 gm; 250 x 4.6 mm), the mobile phase flow rate of 0.8 mL/min and detection at 220 rim. Mobile phases containing hexane and the following modifiers: alcohols (2-propanol, ethanol, methanol) and diethylamine were tested. At first isocratic elution was used but some enantiomers eluted after a long retention time and their peaks were asymmetrical and too wide. Therefore, a gradient elution was developed allow- ing to obtain satisfactory retention times and other parameters of enentioseparation of the compounds.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29513946

Citation

Podolska, Marzena, et al. "HPLC METHOD for SEPARATING ENANTIOMERS of IMIDAZOLE DERIVATIVES - ANTIFUNGAL COMPOUNDS." Acta Poloniae Pharmaceutica, vol. 74, no. 3, 2017, pp. 777-784.
Podolska M, Bialecka W, Kulik A, et al. HPLC METHOD FOR SEPARATING ENANTIOMERS OF IMIDAZOLE DERIVATIVES - ANTIFUNGAL COMPOUNDS. Acta Pol Pharm. 2017;74(3):777-784.
Podolska, M., Bialecka, W., Kulik, A., Kwiatkowska-Puchniarz, B., & Mazurek, A. (2017). HPLC METHOD FOR SEPARATING ENANTIOMERS OF IMIDAZOLE DERIVATIVES - ANTIFUNGAL COMPOUNDS. Acta Poloniae Pharmaceutica, 74(3), pp. 777-784.
Podolska M, et al. HPLC METHOD for SEPARATING ENANTIOMERS of IMIDAZOLE DERIVATIVES - ANTIFUNGAL COMPOUNDS. Acta Pol Pharm. 2017;74(3):777-784. PubMed PMID: 29513946.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HPLC METHOD FOR SEPARATING ENANTIOMERS OF IMIDAZOLE DERIVATIVES - ANTIFUNGAL COMPOUNDS. AU - Podolska,Marzena, AU - Bialecka,Wanda, AU - Kulik,Anna, AU - Kwiatkowska-Puchniarz,Barbara, AU - Mazurek,Aleksander, PY - 2018/3/8/entrez PY - 2018/3/8/pubmed PY - 2018/4/27/medline SP - 777 EP - 784 JF - Acta poloniae pharmaceutica JO - Acta Pol Pharm VL - 74 IS - 3 N2 - The aim of this study was to test separation possibility of enantiomers of nine active substances belonging to imidazole derivatives: bifonazole, butoconazole, econazole, enilconazole, fenticonazole, isoconazole, miconazole, sertaconazole and tioconazole. The study was performed using HPLC method and the CHI- RALCEL OJ column (10 gm; 250 x 4.6 mm), the mobile phase flow rate of 0.8 mL/min and detection at 220 rim. Mobile phases containing hexane and the following modifiers: alcohols (2-propanol, ethanol, methanol) and diethylamine were tested. At first isocratic elution was used but some enantiomers eluted after a long retention time and their peaks were asymmetrical and too wide. Therefore, a gradient elution was developed allow- ing to obtain satisfactory retention times and other parameters of enentioseparation of the compounds. SN - 0001-6837 UR - https://www.unboundmedicine.com/medline/citation/29513946/HPLC_METHOD_FOR_SEPARATING_ENANTIOMERS_OF_IMIDAZOLE_DERIVATIVES_-_ANTIFUNGAL_COMPOUNDS L2 - http://www.ptfarm.pl/pub/File/Acta_Poloniae/2017/3/777.pdf DB - PRIME DP - Unbound Medicine ER -