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Inhibition of protein kinase A affects Paracoccidioides lutzii dimorphism.
Int J Biol Macromol. 2018 Jul 01; 113:1214-1220.IJ

Abstract

A critical step in the lifecycle of many fungal pathogens is the ability to switch between filamentous and yeast growth, a process known as dimorphism. cAMP-dependent protein kinase (PKA) controls morphological changes and the pathogenicity of several animal and plant pathogenic fungi. In this work, we report the analysis of PKA activity during the mycelium to yeast transition in the pathogenic fungus Paracoccidioides lutzii. This fungus, as well as the closely related species Paracoccidioides brasiliensis, causes paracoccidioidomycosis, a systemic mycosis that affects thousands of people in Latin America. Infection occurs when hypha fragments or spores released from mycelium are inhaled by the host, an event that triggers the morphological switch. We show here that PKA activity is regulated in the fungus phase, increasing during the mycelium to yeast transition. Also, morphological transition from mycelium to yeast is blocked by the compound H89, a specific PKA inhibitor. Nevertheless, the fungus recovers its ability to change morphology when H89 is removed from the culture media. This recovery is accompanied by a significant increase in PKA activity. Our results strongly indicate that PKA directly affects phase transition in P. lutzii.

Authors+Show Affiliations

Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Brazil; Unidade Universitária de Ciências Exatas e Tecnológicas, Universidade Estadual de Goiás, Brazil; Centro Universitário de Anápolis, UniEvangélica, Brazil.Centro Universitário de Anápolis, UniEvangélica, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Brazil.Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Brazil. Electronic address: silviaizacc@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29518446

Citation

Sestari, Sheila J., et al. "Inhibition of Protein Kinase a Affects Paracoccidioides Lutzii Dimorphism." International Journal of Biological Macromolecules, vol. 113, 2018, pp. 1214-1220.
Sestari SJ, Brito WA, Neves BJ, et al. Inhibition of protein kinase A affects Paracoccidioides lutzii dimorphism. Int J Biol Macromol. 2018;113:1214-1220.
Sestari, S. J., Brito, W. A., Neves, B. J., Soares, C. M. A., & Salem-Izacc, S. M. (2018). Inhibition of protein kinase A affects Paracoccidioides lutzii dimorphism. International Journal of Biological Macromolecules, 113, 1214-1220. https://doi.org/10.1016/j.ijbiomac.2018.03.023
Sestari SJ, et al. Inhibition of Protein Kinase a Affects Paracoccidioides Lutzii Dimorphism. Int J Biol Macromol. 2018 Jul 1;113:1214-1220. PubMed PMID: 29518446.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of protein kinase A affects Paracoccidioides lutzii dimorphism. AU - Sestari,Sheila J, AU - Brito,Wesley A, AU - Neves,Bruno J, AU - Soares,Celia M A, AU - Salem-Izacc,Silvia M, Y1 - 2018/03/06/ PY - 2017/08/07/received PY - 2018/02/16/revised PY - 2018/03/05/accepted PY - 2018/3/9/pubmed PY - 2018/9/19/medline PY - 2018/3/9/entrez KW - H89 KW - PKA KW - Paracoccidioides lutzii SP - 1214 EP - 1220 JF - International journal of biological macromolecules JO - Int. J. Biol. Macromol. VL - 113 N2 - A critical step in the lifecycle of many fungal pathogens is the ability to switch between filamentous and yeast growth, a process known as dimorphism. cAMP-dependent protein kinase (PKA) controls morphological changes and the pathogenicity of several animal and plant pathogenic fungi. In this work, we report the analysis of PKA activity during the mycelium to yeast transition in the pathogenic fungus Paracoccidioides lutzii. This fungus, as well as the closely related species Paracoccidioides brasiliensis, causes paracoccidioidomycosis, a systemic mycosis that affects thousands of people in Latin America. Infection occurs when hypha fragments or spores released from mycelium are inhaled by the host, an event that triggers the morphological switch. We show here that PKA activity is regulated in the fungus phase, increasing during the mycelium to yeast transition. Also, morphological transition from mycelium to yeast is blocked by the compound H89, a specific PKA inhibitor. Nevertheless, the fungus recovers its ability to change morphology when H89 is removed from the culture media. This recovery is accompanied by a significant increase in PKA activity. Our results strongly indicate that PKA directly affects phase transition in P. lutzii. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/29518446/Inhibition_of_protein_kinase_A_affects_Paracoccidioides_lutzii_dimorphism_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(17)32956-2 DB - PRIME DP - Unbound Medicine ER -