Tags

Type your tag names separated by a space and hit enter

Dual-origin plasmids containing an amplifiable ColE1 ori; temperature-controlled expression of cloned genes.
Gene. 1986; 49(3):311-21.GENE

Abstract

Dual-origin plasmids comprising an inducible ColE1-derived origin of replication controlled by the lambda pR promoter, the cI857 temperature-sensitive repressor gene and the pSC101 origin of replication and its associated par sequence, were constructed. Such plasmids carrying cloned genes were stably maintained at four copies per chromosome, and were readily amplifiable by thermal induction. Cloned gene expression increased with copy number, and accumulation values of greater than 20% total cellular protein were detected. These vectors should prove useful for the production of foreign protein on a large scale, since they provide for stable plasmid maintenance during the growth phase, and high-level gene expression without plasmid loss during the production phase.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2952556

Citation

Wright, E M., et al. "Dual-origin Plasmids Containing an Amplifiable ColE1 Ori; Temperature-controlled Expression of Cloned Genes." Gene, vol. 49, no. 3, 1986, pp. 311-21.
Wright EM, Humphreys GO, Yarranton GT. Dual-origin plasmids containing an amplifiable ColE1 ori; temperature-controlled expression of cloned genes. Gene. 1986;49(3):311-21.
Wright, E. M., Humphreys, G. O., & Yarranton, G. T. (1986). Dual-origin plasmids containing an amplifiable ColE1 ori; temperature-controlled expression of cloned genes. Gene, 49(3), 311-21.
Wright EM, Humphreys GO, Yarranton GT. Dual-origin Plasmids Containing an Amplifiable ColE1 Ori; Temperature-controlled Expression of Cloned Genes. Gene. 1986;49(3):311-21. PubMed PMID: 2952556.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dual-origin plasmids containing an amplifiable ColE1 ori; temperature-controlled expression of cloned genes. AU - Wright,E M, AU - Humphreys,G O, AU - Yarranton,G T, PY - 1986/1/1/pubmed PY - 1986/1/1/medline PY - 1986/1/1/entrez SP - 311 EP - 21 JF - Gene JO - Gene VL - 49 IS - 3 N2 - Dual-origin plasmids comprising an inducible ColE1-derived origin of replication controlled by the lambda pR promoter, the cI857 temperature-sensitive repressor gene and the pSC101 origin of replication and its associated par sequence, were constructed. Such plasmids carrying cloned genes were stably maintained at four copies per chromosome, and were readily amplifiable by thermal induction. Cloned gene expression increased with copy number, and accumulation values of greater than 20% total cellular protein were detected. These vectors should prove useful for the production of foreign protein on a large scale, since they provide for stable plasmid maintenance during the growth phase, and high-level gene expression without plasmid loss during the production phase. SN - 0378-1119 UR - https://www.unboundmedicine.com/medline/citation/2952556/Dual_origin_plasmids_containing_an_amplifiable_ColE1_ori L2 - https://linkinghub.elsevier.com/retrieve/pii/0378-1119(86)90367-7 DB - PRIME DP - Unbound Medicine ER -