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Antioxidant Protection of Nobiletin, 5-Demethylnobiletin, Tangeretin, and 5-Demethyltangeretin from Citrus Peel in Saccharomyces cerevisiae.
J Agric Food Chem. 2018 Mar 28; 66(12):3155-3160.JA

Abstract

Aging and oxidative-related events are closely associated with the oxidative damages induced by excess reactive oxygen species (ROS). The phytochemicals nobiletin (NBT) and tangeretin (TAN) and their 5-demethylated derivatives 5-demethylnobiletin (5-DN) and 5-demethyltangeretin (5-DT) are the representative polymethoxyflavone (PMF) compounds found in aged citrus peels. Although the health benefits from PMFs due to their antioxidant activities have been well documented, a systematic assessment regarding the antioxidation process of PMFs is still lacking attention. Herein, we investigated the effects of the four PMFs subjected to oxidative stress including hydrogen peroxide, carbon tetrachloride, and cadmium sulfate using an emerging model organism Saccharomyces cerevisiae. As expected, all four of the PMFs exhibited improved cellular tolerance with decreasing lipid peroxidation and ROS. Furthermore, by using the mutant strains deficient in catalase, superoxide dismutase, or glutathione synthase, NBT, 5-DN, and TAN appear to contribute to the increased tolerance by activating cytosolic catalase under CCl4, while the antioxidant protection conferred by 5-DT against H2O2 and CdSO4 seems to require cytosolic catalase and glutathione, respectively. However, the involvement of Ctt1 and Sod1 is achieved neither by decreasing lipid peroxidation nor by scavenging intracellular ROS according to our results. In addition, a comparison of antioxidant capability of the four PMFs was conducted in this study. In general, this research tries to explore the antioxidant mechanism of PMFs in Saccharomyces cerevisiae, hoping to provide an example for developing more efficacious dietary antioxidants to battle against oxidative- or age-related illness.

Authors+Show Affiliations

Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science , Tianjin University of Commerce , Tianjin 300134 , China.Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science , Tianjin University of Commerce , Tianjin 300134 , China.Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science , Tianjin University of Commerce , Tianjin 300134 , China. Hubei Key Laboratory for Processing & Application of Catalytic Materials, College of Chemistry & Chemical Engineering , Huanggang Normal University , No. 146 Xingang 2 Road , Huanggang , Hubei 438000 , China.Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science , Tianjin University of Commerce , Tianjin 300134 , China.Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science , Tianjin University of Commerce , Tianjin 300134 , China.Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science , Tianjin University of Commerce , Tianjin 300134 , China. Centre for Food Research and Innovation, Department of Wine, Food and Molecular Bioscience , Lincoln University , Lincoln 7647 , New Zealand.Hubei Key Laboratory for Processing & Application of Catalytic Materials, College of Chemistry & Chemical Engineering , Huanggang Normal University , No. 146 Xingang 2 Road , Huanggang , Hubei 438000 , China. Department of Food Science , Rutgers University , New Brunswick , New Jersey 08901 , United States.Department of Food Science , Rutgers University , New Brunswick , New Jersey 08901 , United States.Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science , Tianjin University of Commerce , Tianjin 300134 , China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29526093

Citation

Wang, Meiyan, et al. "Antioxidant Protection of Nobiletin, 5-Demethylnobiletin, Tangeretin, and 5-Demethyltangeretin From Citrus Peel in Saccharomyces Cerevisiae." Journal of Agricultural and Food Chemistry, vol. 66, no. 12, 2018, pp. 3155-3160.
Wang M, Meng D, Zhang P, et al. Antioxidant Protection of Nobiletin, 5-Demethylnobiletin, Tangeretin, and 5-Demethyltangeretin from Citrus Peel in Saccharomyces cerevisiae. J Agric Food Chem. 2018;66(12):3155-3160.
Wang, M., Meng, D., Zhang, P., Wang, X., Du, G., Brennan, C., Li, S., Ho, C. T., & Zhao, H. (2018). Antioxidant Protection of Nobiletin, 5-Demethylnobiletin, Tangeretin, and 5-Demethyltangeretin from Citrus Peel in Saccharomyces cerevisiae. Journal of Agricultural and Food Chemistry, 66(12), 3155-3160. https://doi.org/10.1021/acs.jafc.8b00509
Wang M, et al. Antioxidant Protection of Nobiletin, 5-Demethylnobiletin, Tangeretin, and 5-Demethyltangeretin From Citrus Peel in Saccharomyces Cerevisiae. J Agric Food Chem. 2018 Mar 28;66(12):3155-3160. PubMed PMID: 29526093.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant Protection of Nobiletin, 5-Demethylnobiletin, Tangeretin, and 5-Demethyltangeretin from Citrus Peel in Saccharomyces cerevisiae. AU - Wang,Meiyan, AU - Meng,Dan, AU - Zhang,Peng, AU - Wang,Xiangxing, AU - Du,Gang, AU - Brennan,Charles, AU - Li,Shiming, AU - Ho,Chi-Tang, AU - Zhao,Hui, Y1 - 2018/03/16/ PY - 2018/3/13/pubmed PY - 2018/4/17/medline PY - 2018/3/13/entrez KW - PMF KW - ROS KW - Saccharomyces cerevisiae KW - antioxidant KW - lipid peroxidation SP - 3155 EP - 3160 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 66 IS - 12 N2 - Aging and oxidative-related events are closely associated with the oxidative damages induced by excess reactive oxygen species (ROS). The phytochemicals nobiletin (NBT) and tangeretin (TAN) and their 5-demethylated derivatives 5-demethylnobiletin (5-DN) and 5-demethyltangeretin (5-DT) are the representative polymethoxyflavone (PMF) compounds found in aged citrus peels. Although the health benefits from PMFs due to their antioxidant activities have been well documented, a systematic assessment regarding the antioxidation process of PMFs is still lacking attention. Herein, we investigated the effects of the four PMFs subjected to oxidative stress including hydrogen peroxide, carbon tetrachloride, and cadmium sulfate using an emerging model organism Saccharomyces cerevisiae. As expected, all four of the PMFs exhibited improved cellular tolerance with decreasing lipid peroxidation and ROS. Furthermore, by using the mutant strains deficient in catalase, superoxide dismutase, or glutathione synthase, NBT, 5-DN, and TAN appear to contribute to the increased tolerance by activating cytosolic catalase under CCl4, while the antioxidant protection conferred by 5-DT against H2O2 and CdSO4 seems to require cytosolic catalase and glutathione, respectively. However, the involvement of Ctt1 and Sod1 is achieved neither by decreasing lipid peroxidation nor by scavenging intracellular ROS according to our results. In addition, a comparison of antioxidant capability of the four PMFs was conducted in this study. In general, this research tries to explore the antioxidant mechanism of PMFs in Saccharomyces cerevisiae, hoping to provide an example for developing more efficacious dietary antioxidants to battle against oxidative- or age-related illness. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/29526093/Antioxidant_Protection_of_Nobiletin_5_Demethylnobiletin_Tangeretin_and_5_Demethyltangeretin_from_Citrus_Peel_in_Saccharomyces_cerevisiae_ L2 - https://doi.org/10.1021/acs.jafc.8b00509 DB - PRIME DP - Unbound Medicine ER -