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Medicinal properties of Ocotea bullata stem bark extracts: phytochemical constituents, antioxidant and anti-inflammatory activity, cytotoxicity and inhibition of carbohydrate-metabolizing enzymes.
J Integr Med. 2018 03; 16(2):132-140.JI

Abstract

OBJECTIVE

This study evaluates the phytochemical constituents, antioxidant and anti-inflammatory activity, cytotoxicity, and inhibitory activity against carbohydrate metabolism of extracts from Ocotea bullata stem bark.

METHODS

Hexane, ethyl acetate, methanol and water were used to extract the air-dried sample. The phytochemical investigation and antioxidant assays were carried out on the extracts using standard procedures. The antidiabetic and anti-inflammatory potentials were evaluated using α-amylase, α-glucosidase and 5-lipoxygenase enzymes respectively. Vero cells were employed to determine the cytotoxicity of the extracts.

RESULTS

The ethyl acetate extract showed higher phenolic contents (8.97 mg/g gallic acid) while methanol displayed higher flavonoid (36.06 mg/g quercetin) and flavonol (153.44 mg/g rutin) contents than other extracts. Hexane extract had the greatest capacity to scavenge 1,1-diphenyl-2-picryl-hydrazyl (0.19 mg/mL), hydroxyl (25.77 mg/mL) and 2,2-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid (0.07 mg/mL) radicals, while ethyl acetate extract exhibited stronger inhibition (P < 0.05) against superoxide anion (0.41 mg/mL) and ferric ion-reducing power (2.36 mg/mL) compared to other extracts and standards. Aqueous extract (27.02 mg/mL) exhibited strong metal-chelating activity (P < 0.05) compared to other extracts and gallic acid. The aqueous extract demonstrated the greatest inhibition of α-glucosidase (1.45 mg/mL) and α-amylase (2.43 mg/mL) compared to other extracts and acarbose. There were no significant differences (P < 0.05) in half-maximum inhibitory concentration (IC50) values of all tested extracts and indomethacin in the inhibition of 5-lipoxygenase activity. The aqueous extract was nontoxic to Vero cells with an IC50 value of 0.38 mg/mL.

CONCLUSION

O. bullata stem bark contains active phytochemicals with diverse pharmacological potentials that could be beneficial in managing diabetes and inflammation.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Ogundajo AL
Phytomedicine and Phytopharmacology Research Group, Department of Plant Sciences, University of the Free State, Qwaqwa Campus, Phuthaditjhaba 9866, South Africa.
Adeniran LA
Phytomedicine and Phytopharmacology Research Group, Department of Plant Sciences, University of the Free State, Qwaqwa Campus, Phuthaditjhaba 9866, South Africa.
Ashafa AO
Phytomedicine and Phytopharmacology Research Group, Department of Plant Sciences, University of the Free State, Qwaqwa Campus, Phuthaditjhaba 9866, South Africa. Electronic address: ashafaaot@ufs.ac.za.

MeSH

AnimalsAnti-Inflammatory AgentsAnti-Obesity AgentsAntioxidantsCell SurvivalChlorocebus aethiopsEnzyme InhibitorsGlycoside Hydrolase InhibitorsHumansKineticsOcoteaPlant BarkPlant ExtractsVero Cellsalpha-Amylasesalpha-Glucosidases

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29526237

Citation

Ogundajo, Akintayo Lanre, et al. "Medicinal Properties of Ocotea Bullata Stem Bark Extracts: Phytochemical Constituents, Antioxidant and Anti-inflammatory Activity, Cytotoxicity and Inhibition of Carbohydrate-metabolizing Enzymes." Journal of Integrative Medicine, vol. 16, no. 2, 2018, pp. 132-140.
Ogundajo AL, Adeniran LA, Ashafa AO. Medicinal properties of Ocotea bullata stem bark extracts: phytochemical constituents, antioxidant and anti-inflammatory activity, cytotoxicity and inhibition of carbohydrate-metabolizing enzymes. J Integr Med. 2018;16(2):132-140.
Ogundajo, A. L., Adeniran, L. A., & Ashafa, A. O. (2018). Medicinal properties of Ocotea bullata stem bark extracts: phytochemical constituents, antioxidant and anti-inflammatory activity, cytotoxicity and inhibition of carbohydrate-metabolizing enzymes. Journal of Integrative Medicine, 16(2), 132-140. https://doi.org/10.1016/j.joim.2018.02.007
Ogundajo AL, Adeniran LA, Ashafa AO. Medicinal Properties of Ocotea Bullata Stem Bark Extracts: Phytochemical Constituents, Antioxidant and Anti-inflammatory Activity, Cytotoxicity and Inhibition of Carbohydrate-metabolizing Enzymes. J Integr Med. 2018;16(2):132-140. PubMed PMID: 29526237.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Medicinal properties of Ocotea bullata stem bark extracts: phytochemical constituents, antioxidant and anti-inflammatory activity, cytotoxicity and inhibition of carbohydrate-metabolizing enzymes. AU - Ogundajo,Akintayo Lanre, AU - Adeniran,Lateef Apollo, AU - Ashafa,Anofi Omotayo, Y1 - 2018/02/12/ PY - 2017/04/14/received PY - 2017/09/26/accepted PY - 2018/3/13/entrez PY - 2018/3/13/pubmed PY - 2018/10/23/medline KW - Anti-inflammatories KW - Antidiabetics KW - Antioxidants KW - Cytotoxicity KW - Ocotea bullata SP - 132 EP - 140 JF - Journal of integrative medicine JO - J Integr Med VL - 16 IS - 2 N2 - OBJECTIVE: This study evaluates the phytochemical constituents, antioxidant and anti-inflammatory activity, cytotoxicity, and inhibitory activity against carbohydrate metabolism of extracts from Ocotea bullata stem bark. METHODS: Hexane, ethyl acetate, methanol and water were used to extract the air-dried sample. The phytochemical investigation and antioxidant assays were carried out on the extracts using standard procedures. The antidiabetic and anti-inflammatory potentials were evaluated using α-amylase, α-glucosidase and 5-lipoxygenase enzymes respectively. Vero cells were employed to determine the cytotoxicity of the extracts. RESULTS: The ethyl acetate extract showed higher phenolic contents (8.97 mg/g gallic acid) while methanol displayed higher flavonoid (36.06 mg/g quercetin) and flavonol (153.44 mg/g rutin) contents than other extracts. Hexane extract had the greatest capacity to scavenge 1,1-diphenyl-2-picryl-hydrazyl (0.19 mg/mL), hydroxyl (25.77 mg/mL) and 2,2-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid (0.07 mg/mL) radicals, while ethyl acetate extract exhibited stronger inhibition (P < 0.05) against superoxide anion (0.41 mg/mL) and ferric ion-reducing power (2.36 mg/mL) compared to other extracts and standards. Aqueous extract (27.02 mg/mL) exhibited strong metal-chelating activity (P < 0.05) compared to other extracts and gallic acid. The aqueous extract demonstrated the greatest inhibition of α-glucosidase (1.45 mg/mL) and α-amylase (2.43 mg/mL) compared to other extracts and acarbose. There were no significant differences (P < 0.05) in half-maximum inhibitory concentration (IC50) values of all tested extracts and indomethacin in the inhibition of 5-lipoxygenase activity. The aqueous extract was nontoxic to Vero cells with an IC50 value of 0.38 mg/mL. CONCLUSION: O. bullata stem bark contains active phytochemicals with diverse pharmacological potentials that could be beneficial in managing diabetes and inflammation. SN - 2095-4964 UR - https://www.unboundmedicine.com/medline/citation/29526237/Medicinal_properties_of_Ocotea_bullata_stem_bark_extracts:_phytochemical_constituents_antioxidant_and_anti_inflammatory_activity_cytotoxicity_and_inhibition_of_carbohydrate_metabolizing_enzymes_ DB - PRIME DP - Unbound Medicine ER -
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