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Characterization and expression analysis of g- and c-type lysozymes in Dabry's sturgeon (Acipenser dabryanus).
Fish Shellfish Immunol. 2018 May; 76:260-265.FS

Abstract

Dabry's sturgeon (Acipenser dabryanus) is mainly distributed in the upper Yangtze River. Although extensively farmed, little information is available on its innate immune system. In this study, we conducted de novo transcriptome assembly of the head kidney to create a comprehensive dataset for A. dabryanus. A total of 51,324,686 high quality reads were obtained from head kidney cDNA library by the Illumina sequencing platform and 131,261 unigenes were determined to contain complete ORFs. The complete coding sequences of g- and c-type lysozymes were identified from unigenes, and designated as ADLysG and ADLysC. Aeromonas hydrophila infection of Dabry's sturgeon caused a significant increase (P < 0.05) in blood for both lysozyme types, confirming their active defensive role against bacterial infections. This research provides the first characterization of these enzymes in an ancestral chondrostean. These data suggest that ADLysG and ADLysC have the potential for immune defense system against bacterial infection.

Authors+Show Affiliations

Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.School of Animal Science, Yangtze University, Jingzhou, 434020, China; Department of Biology, University of Padova, I-35131 Padova, Italy.Department of Biology, University of Padova, I-35131 Padova, Italy.Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.School of Animal Science, Yangtze University, Jingzhou, 434020, China.School of Animal Science, Yangtze University, Jingzhou, 434020, China.Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; School of Animal Science, Yangtze University, Jingzhou, 434020, China.Department of Biology, University of Padova, I-35131 Padova, Italy. Electronic address: leonardo.congiu@unipd.it.Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China; School of Animal Science, Yangtze University, Jingzhou, 434020, China. Electronic address: weiqw@yfi.ac.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29526699

Citation

Zhang, Shuhuan, et al. "Characterization and Expression Analysis of G- and C-type Lysozymes in Dabry's Sturgeon (Acipenser Dabryanus)." Fish & Shellfish Immunology, vol. 76, 2018, pp. 260-265.
Zhang S, Xu Q, Boscari E, et al. Characterization and expression analysis of g- and c-type lysozymes in Dabry's sturgeon (Acipenser dabryanus). Fish Shellfish Immunol. 2018;76:260-265.
Zhang, S., Xu, Q., Boscari, E., Du, H., Qi, Z., Li, Y., Huang, J., Di, J., Yue, H., Li, C., Congiu, L., & Wei, Q. (2018). Characterization and expression analysis of g- and c-type lysozymes in Dabry's sturgeon (Acipenser dabryanus). Fish & Shellfish Immunology, 76, 260-265. https://doi.org/10.1016/j.fsi.2018.03.006
Zhang S, et al. Characterization and Expression Analysis of G- and C-type Lysozymes in Dabry's Sturgeon (Acipenser Dabryanus). Fish Shellfish Immunol. 2018;76:260-265. PubMed PMID: 29526699.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization and expression analysis of g- and c-type lysozymes in Dabry's sturgeon (Acipenser dabryanus). AU - Zhang,Shuhuan, AU - Xu,Qiaoqing, AU - Boscari,Elisa, AU - Du,Hao, AU - Qi,Zhitao, AU - Li,Youshen, AU - Huang,Jun, AU - Di,Jun, AU - Yue,Huamei, AU - Li,Chuangju, AU - Congiu,Leonardo, AU - Wei,Qiwei, Y1 - 2018/03/09/ PY - 2017/10/31/received PY - 2018/02/26/revised PY - 2018/03/02/accepted PY - 2018/3/13/pubmed PY - 2018/9/5/medline PY - 2018/3/13/entrez KW - Aeromonas hydrophila KW - Innate immunity KW - Lysozyme KW - Sturgeon KW - Transcriptome SP - 260 EP - 265 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol. VL - 76 N2 - Dabry's sturgeon (Acipenser dabryanus) is mainly distributed in the upper Yangtze River. Although extensively farmed, little information is available on its innate immune system. In this study, we conducted de novo transcriptome assembly of the head kidney to create a comprehensive dataset for A. dabryanus. A total of 51,324,686 high quality reads were obtained from head kidney cDNA library by the Illumina sequencing platform and 131,261 unigenes were determined to contain complete ORFs. The complete coding sequences of g- and c-type lysozymes were identified from unigenes, and designated as ADLysG and ADLysC. Aeromonas hydrophila infection of Dabry's sturgeon caused a significant increase (P < 0.05) in blood for both lysozyme types, confirming their active defensive role against bacterial infections. This research provides the first characterization of these enzymes in an ancestral chondrostean. These data suggest that ADLysG and ADLysC have the potential for immune defense system against bacterial infection. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/29526699/Characterization_and_expression_analysis_of_g__and_c_type_lysozymes_in_Dabry's_sturgeon__Acipenser_dabryanus__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(18)30123-2 DB - PRIME DP - Unbound Medicine ER -