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LncRNA SNHG16 Functions as an Oncogene by Sponging MiR-4518 and Up-Regulating PRMT5 Expression in Glioma.
Cell Physiol Biochem. 2018; 45(5):1975-1985.CP

Abstract

BACKGROUND/AIMS

Long noncoding RNAs (lncRNAs) have recently emerged as novel and potentially promising therapeutic targets in various cancers. However, the expression pattern and biological function of lncRNAs in glioma remain largely elusive. In the present study, we investigated the functional role of an lncRNA, small nucleolar RNA host gene 16 (SNHG16), in glioma.

METHODS

The expression levels of SNHG16 and miR-4518 were measured using qRT-PCR. The relationship between the levels of SNHG16 and clinicopathologic features were statically analyzed. The levels of proteins were detected using western blot. Bioinformatics analysis and luciferase reporter assays were applied to the analysis of the relationship between SNHG16, miR-4518 and PRMT5. Cell viability and apoptosis were measured using MTT and apoptosis ELISA assay, respectively.

RESULTS

SNHG16 was highly expressed in glioma tissues and cell lines, which was related to poorer clinicopathologic features and shorter survival time. Knockdown of SNHG16 inhibits the viability and induces apoptosis of glioma cells. Further investigation revealed that SNHG16 could up-regulate the expression of miR-4518 targeted gene PRMT5 via acting as an endogenous sponge of miR-4518. Moreover, SNHG16 also affects the expression of Bcl-2 family proteins and the activation of PI3K/Akt signaling pathway.

CONCLUSION

Our study revealed a novel SNHG16-miR-4518-PRMT5 pathway regulatory axis in glioma pathogenesis. SNHG16 could be used as a potential therapeutic target in the treatment of glioma.

Authors+Show Affiliations

Department of Neurology, The Fifth Affiliated Hospital of Wenzhou Medical University, Lishui, China.Department of Neurology, The Fifth Affiliated Hospital of Wenzhou Medical University, Lishui, China.Department of Neurology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.Department of Otorhinolaryngology, The sixth Affiliated Hospital of Wenzhou Medical University, Lishui, China.Department of Neurology, The Fifth Affiliated Hospital of Wenzhou Medical University, Lishui, China.Department of Neurology, The Fifth Affiliated Hospital of Wenzhou Medical University, Lishui, China.Department of Neurology, The Fifth Affiliated Hospital of Wenzhou Medical University, Lishui, China.Department of Neurology, The Fifth Affiliated Hospital of Wenzhou Medical University, Lishui, China.Department of Neurology, The Fifth Affiliated Hospital of Wenzhou Medical University, Lishui, China.Department of Otorhinolaryngology, The sixth Affiliated Hospital of Wenzhou Medical University, Lishui, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29529599

Citation

Lu, Ye-Fen, et al. "LncRNA SNHG16 Functions as an Oncogene By Sponging MiR-4518 and Up-Regulating PRMT5 Expression in Glioma." Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology, vol. 45, no. 5, 2018, pp. 1975-1985.
Lu YF, Cai XL, Li ZZ, et al. LncRNA SNHG16 Functions as an Oncogene by Sponging MiR-4518 and Up-Regulating PRMT5 Expression in Glioma. Cell Physiol Biochem. 2018;45(5):1975-1985.
Lu, Y. F., Cai, X. L., Li, Z. Z., Lv, J., Xiang, Y. A., Chen, J. J., Chen, W. J., Sun, W. Y., Liu, X. M., & Chen, J. B. (2018). LncRNA SNHG16 Functions as an Oncogene by Sponging MiR-4518 and Up-Regulating PRMT5 Expression in Glioma. Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology, 45(5), 1975-1985. https://doi.org/10.1159/000487974
Lu YF, et al. LncRNA SNHG16 Functions as an Oncogene By Sponging MiR-4518 and Up-Regulating PRMT5 Expression in Glioma. Cell Physiol Biochem. 2018;45(5):1975-1985. PubMed PMID: 29529599.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - LncRNA SNHG16 Functions as an Oncogene by Sponging MiR-4518 and Up-Regulating PRMT5 Expression in Glioma. AU - Lu,Ye-Fen, AU - Cai,Xue-Li, AU - Li,Zheng-Zheng, AU - Lv,Jing, AU - Xiang,Yi-An, AU - Chen,Jian-Jun, AU - Chen,Wei-Jing, AU - Sun,Wei-Yan, AU - Liu,Xiu-Mei, AU - Chen,Jian-Bo, Y1 - 2018/03/06/ PY - 2017/10/19/received PY - 2018/01/16/accepted PY - 2018/3/13/pubmed PY - 2018/5/15/medline PY - 2018/3/13/entrez KW - Glioma KW - Long non-coding RNA KW - MiR-4518 KW - PRMT5 KW - SNHG16 SP - 1975 EP - 1985 JF - Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology JO - Cell. Physiol. Biochem. VL - 45 IS - 5 N2 - BACKGROUND/AIMS: Long noncoding RNAs (lncRNAs) have recently emerged as novel and potentially promising therapeutic targets in various cancers. However, the expression pattern and biological function of lncRNAs in glioma remain largely elusive. In the present study, we investigated the functional role of an lncRNA, small nucleolar RNA host gene 16 (SNHG16), in glioma. METHODS: The expression levels of SNHG16 and miR-4518 were measured using qRT-PCR. The relationship between the levels of SNHG16 and clinicopathologic features were statically analyzed. The levels of proteins were detected using western blot. Bioinformatics analysis and luciferase reporter assays were applied to the analysis of the relationship between SNHG16, miR-4518 and PRMT5. Cell viability and apoptosis were measured using MTT and apoptosis ELISA assay, respectively. RESULTS: SNHG16 was highly expressed in glioma tissues and cell lines, which was related to poorer clinicopathologic features and shorter survival time. Knockdown of SNHG16 inhibits the viability and induces apoptosis of glioma cells. Further investigation revealed that SNHG16 could up-regulate the expression of miR-4518 targeted gene PRMT5 via acting as an endogenous sponge of miR-4518. Moreover, SNHG16 also affects the expression of Bcl-2 family proteins and the activation of PI3K/Akt signaling pathway. CONCLUSION: Our study revealed a novel SNHG16-miR-4518-PRMT5 pathway regulatory axis in glioma pathogenesis. SNHG16 could be used as a potential therapeutic target in the treatment of glioma. SN - 1421-9778 UR - https://www.unboundmedicine.com/medline/citation/29529599/LncRNA_SNHG16_Functions_as_an_Oncogene_by_Sponging_MiR_4518_and_Up_Regulating_PRMT5_Expression_in_Glioma_ L2 - https://www.karger.com?DOI=10.1159/000487974 DB - PRIME DP - Unbound Medicine ER -