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Detectability of fentanyl and designer fentanyls in urine by 3 commercial fentanyl immunoassays.
Drug Test Anal. 2018 Mar 12 [Online ahead of print]DT

Abstract

In recent times, structural variants of fentanyl (designer fentanyls) have appeared on the recreational drug market for new psychoactive substances (NPS). These potent opioids have caused harmful intoxications and increased opioid-related mortality in many countries. This work evaluated 3 commercial immunoassays for fentanyl screening in urine and investigated whether they are useful also for screening of designer fentanyls. The assays examined were the Thermo DRI® Fentanyl Enzyme Immunoassay, the ARK™ Fentanyl Assay homogeneous enzyme immunoassay, and the Immunalysis® Fentanyl Urine SEFRIA™ Drug Screening Kit. A liquid chromatography-high-resolution mass spectrometry method was used as reference. The DRI fentanyl immunoassay generated somewhat higher assay imprecision values (%CV) compared with the ARK™ and SEFRIA™ assays, but all assays showed %CV values acceptable for routine use. The 3 assays showed overall good detectability (33%-95% cross-reactivity) for blank urine samples spiked with acetylfentanyl, acrylfentanyl, butyrfentanyl, 4-chloroisobutyrfentanyl, 4-fluorobutyrfentanyl, 4-fluorofentanyl, 4-fluoroisobutyrfentnyl, isobutyrfentanyl, methoxyacetylfentanyl, or tetrahydrofuranfentanyl, whereas 4-methoxybutyrfentanyl (all assays) and 2-fluorofentanyl (DRI assay) showed low cross-reactivity. A good detectability of designer fentanyls was confirmed in urine samples from authentic acute intoxications. In conclusion, the present results demonstrate that the urinary fentanyl immunoassays are generally useful also for preliminary screening of fentanyl analogs sold as NPS. When the SEFRIA™ assay was applied for testing of 980 urine samples from patients treated for drug dependence in Sweden, only 1 sample was confirmed positive for fentanyl.

Authors+Show Affiliations

Department of Laboratory Medicine, Division of Clinical Pharmacology, Karolinska Institutet, Stockholm, Sweden. Department of Laboratory Medicine, Division of Clinical Chemistry, Karolinska Institutet, Stockholm, Sweden. Department of Clinical Pharmacology, Karolinska University Laboratory, Stockholm, Sweden.Department of Clinical Pharmacology, Karolinska University Laboratory, Stockholm, Sweden.Department of Clinical Pharmacology, Karolinska University Laboratory, Stockholm, Sweden.Department of Laboratory Medicine, Division of Clinical Pharmacology, Karolinska Institutet, Stockholm, Sweden. Department of Clinical Pharmacology, Karolinska University Laboratory, Stockholm, Sweden.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29529707

Citation

Helander, Anders, et al. "Detectability of Fentanyl and Designer Fentanyls in Urine By 3 Commercial Fentanyl Immunoassays." Drug Testing and Analysis, 2018.
Helander A, Stojanovic K, Villén T, et al. Detectability of fentanyl and designer fentanyls in urine by 3 commercial fentanyl immunoassays. Drug Test Anal. 2018.
Helander, A., Stojanovic, K., Villén, T., & Beck, O. (2018). Detectability of fentanyl and designer fentanyls in urine by 3 commercial fentanyl immunoassays. Drug Testing and Analysis. https://doi.org/10.1002/dta.2382
Helander A, et al. Detectability of Fentanyl and Designer Fentanyls in Urine By 3 Commercial Fentanyl Immunoassays. Drug Test Anal. 2018 Mar 12; PubMed PMID: 29529707.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detectability of fentanyl and designer fentanyls in urine by 3 commercial fentanyl immunoassays. AU - Helander,Anders, AU - Stojanovic,Katarina, AU - Villén,Tomas, AU - Beck,Olof, Y1 - 2018/03/12/ PY - 2018/01/29/received PY - 2018/03/06/revised PY - 2018/03/06/accepted PY - 2018/3/13/pubmed PY - 2018/3/13/medline PY - 2018/3/13/entrez KW - Drug testing KW - fentanyl analogs KW - immunoassays KW - new psychoactive substances KW - urine JF - Drug testing and analysis JO - Drug Test Anal N2 - In recent times, structural variants of fentanyl (designer fentanyls) have appeared on the recreational drug market for new psychoactive substances (NPS). These potent opioids have caused harmful intoxications and increased opioid-related mortality in many countries. This work evaluated 3 commercial immunoassays for fentanyl screening in urine and investigated whether they are useful also for screening of designer fentanyls. The assays examined were the Thermo DRI® Fentanyl Enzyme Immunoassay, the ARK™ Fentanyl Assay homogeneous enzyme immunoassay, and the Immunalysis® Fentanyl Urine SEFRIA™ Drug Screening Kit. A liquid chromatography-high-resolution mass spectrometry method was used as reference. The DRI fentanyl immunoassay generated somewhat higher assay imprecision values (%CV) compared with the ARK™ and SEFRIA™ assays, but all assays showed %CV values acceptable for routine use. The 3 assays showed overall good detectability (33%-95% cross-reactivity) for blank urine samples spiked with acetylfentanyl, acrylfentanyl, butyrfentanyl, 4-chloroisobutyrfentanyl, 4-fluorobutyrfentanyl, 4-fluorofentanyl, 4-fluoroisobutyrfentnyl, isobutyrfentanyl, methoxyacetylfentanyl, or tetrahydrofuranfentanyl, whereas 4-methoxybutyrfentanyl (all assays) and 2-fluorofentanyl (DRI assay) showed low cross-reactivity. A good detectability of designer fentanyls was confirmed in urine samples from authentic acute intoxications. In conclusion, the present results demonstrate that the urinary fentanyl immunoassays are generally useful also for preliminary screening of fentanyl analogs sold as NPS. When the SEFRIA™ assay was applied for testing of 980 urine samples from patients treated for drug dependence in Sweden, only 1 sample was confirmed positive for fentanyl. SN - 1942-7611 UR - https://www.unboundmedicine.com/medline/citation/29529707/Detectability_of_fentanyl_and_designer_fentanyls_in_urine_by_3_commercial_fentanyl_immunoassays_ L2 - https://doi.org/10.1002/dta.2382 DB - PRIME DP - Unbound Medicine ER -
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