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The plasma interleukin-6 response to acute psychosocial stress in humans is detected by a magnetic multiplex assay: comparison to high-sensitivity ELISA.
Stress. 2018 07; 21(4):376-381.S

Abstract

Circulating concentrations of interleukin (IL)-6, an inflammatory biomarker widely assessed in humans to study the inflammatory response to acute psychological stress, have for decades been quantified using enzyme-linked immunosorbent assay (ELISA). However, biobehavioral researchers are increasingly using cytokine multiplex assays instead of ELISA to measure IL-6 and other cytokines. Despite this trend, multiplex assays have not been directly compared to ELISA for their ability to detect subtle stress-induced changes of IL-6. Here, we tested the prediction that a high-sensitivity multiplex assay (human Magnetic Luminex Performance Assay, R&D Systems, Minneapolis, MN) would detect changes in IL-6 as a result of acute stress challenge in a manner comparable to high-sensitivity ELISA. Blood was collected from 12 healthy adults immediately before and then 90 and 210 min after the start of the Trier Social Stress Test (TSST), an acute laboratory psychosocial stress challenge. In addition to quantifying IL-6 concentrations in plasma with both multiplex and ELISA, we also assessed concentrations of tumor necrosis factor-alpha, IL-8, IL-10, IL-5, and IL-2 with multiplex. The multiplex detected IL-6 in all samples. Concentrations strongly correlated with values determined by ELISA across all samples (r = 0.941, p < .001) as well as among samples collected at individual TSST time points. IL-6 responses to the TSST (i.e. area under the curve) captured by multiplex and ELISA were also strongly correlated (rs = 0.937, p < .001). While other cytokines were detected by multiplex, none changed as a result of TSST challenge at time points examined. These results suggest high-sensitivity magnetic multiplex assay is able to detect changes in plasma concentrations of IL-6 as a result of acute stress in humans.

Authors+Show Affiliations

a Division of Community and Systems Health Science, College of Nursing , University of Arizona , Tucson , AZ , USA.b Department of Psychiatry and Behavioral Sciences , Emory University School of Medicine , Atlanta , GA , USA.b Department of Psychiatry and Behavioral Sciences , Emory University School of Medicine , Atlanta , GA , USA.c Human Development and Family Studies, School of Human Ecology and Department of Psychiatry, School of Medicine and Public Health , University of Wisconsin-Madison , Madison , WI , USA.a Division of Community and Systems Health Science, College of Nursing , University of Arizona , Tucson , AZ , USA.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

29529950

Citation

Quinn, Andrea M., et al. "The Plasma Interleukin-6 Response to Acute Psychosocial Stress in Humans Is Detected By a Magnetic Multiplex Assay: Comparison to High-sensitivity ELISA." Stress (Amsterdam, Netherlands), vol. 21, no. 4, 2018, pp. 376-381.
Quinn AM, Williams AR, Sivilli TI, et al. The plasma interleukin-6 response to acute psychosocial stress in humans is detected by a magnetic multiplex assay: comparison to high-sensitivity ELISA. Stress. 2018;21(4):376-381.
Quinn, A. M., Williams, A. R., Sivilli, T. I., Raison, C. L., & Pace, T. W. W. (2018). The plasma interleukin-6 response to acute psychosocial stress in humans is detected by a magnetic multiplex assay: comparison to high-sensitivity ELISA. Stress (Amsterdam, Netherlands), 21(4), 376-381. https://doi.org/10.1080/10253890.2018.1446518
Quinn AM, et al. The Plasma Interleukin-6 Response to Acute Psychosocial Stress in Humans Is Detected By a Magnetic Multiplex Assay: Comparison to High-sensitivity ELISA. Stress. 2018;21(4):376-381. PubMed PMID: 29529950.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The plasma interleukin-6 response to acute psychosocial stress in humans is detected by a magnetic multiplex assay: comparison to high-sensitivity ELISA. AU - Quinn,Andrea M, AU - Williams,Allison R, AU - Sivilli,Teresa I, AU - Raison,Charles L, AU - Pace,Thaddeus W W, Y1 - 2018/03/13/ PY - 2018/3/14/pubmed PY - 2019/2/12/medline PY - 2018/3/14/entrez KW - Acute stress KW - ELISA KW - TSST KW - human KW - interleukin-6 KW - multiplex SP - 376 EP - 381 JF - Stress (Amsterdam, Netherlands) JO - Stress VL - 21 IS - 4 N2 - Circulating concentrations of interleukin (IL)-6, an inflammatory biomarker widely assessed in humans to study the inflammatory response to acute psychological stress, have for decades been quantified using enzyme-linked immunosorbent assay (ELISA). However, biobehavioral researchers are increasingly using cytokine multiplex assays instead of ELISA to measure IL-6 and other cytokines. Despite this trend, multiplex assays have not been directly compared to ELISA for their ability to detect subtle stress-induced changes of IL-6. Here, we tested the prediction that a high-sensitivity multiplex assay (human Magnetic Luminex Performance Assay, R&D Systems, Minneapolis, MN) would detect changes in IL-6 as a result of acute stress challenge in a manner comparable to high-sensitivity ELISA. Blood was collected from 12 healthy adults immediately before and then 90 and 210 min after the start of the Trier Social Stress Test (TSST), an acute laboratory psychosocial stress challenge. In addition to quantifying IL-6 concentrations in plasma with both multiplex and ELISA, we also assessed concentrations of tumor necrosis factor-alpha, IL-8, IL-10, IL-5, and IL-2 with multiplex. The multiplex detected IL-6 in all samples. Concentrations strongly correlated with values determined by ELISA across all samples (r = 0.941, p < .001) as well as among samples collected at individual TSST time points. IL-6 responses to the TSST (i.e. area under the curve) captured by multiplex and ELISA were also strongly correlated (rs = 0.937, p < .001). While other cytokines were detected by multiplex, none changed as a result of TSST challenge at time points examined. These results suggest high-sensitivity magnetic multiplex assay is able to detect changes in plasma concentrations of IL-6 as a result of acute stress in humans. SN - 1607-8888 UR - https://www.unboundmedicine.com/medline/citation/29529950/The_plasma_interleukin_6_response_to_acute_psychosocial_stress_in_humans_is_detected_by_a_magnetic_multiplex_assay:_comparison_to_high_sensitivity_ELISA_ L2 - http://www.tandfonline.com/doi/full/10.1080/10253890.2018.1446518 DB - PRIME DP - Unbound Medicine ER -