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In vitro investigation of cytotoxic and antioxidative activities of Ardisia crispa against breast cancer cell lines, MCF-7 and MDA-MB-231.
BMC Complement Altern Med. 2018 Mar 12; 18(1):87.BC

Abstract

BACKGROUND

Ardisia crispa Thunb. D.C is used mostly in some parts of the Asian region by traditional practitioners to treat certain diseases associated with oxidative stress and inflammation including cancer and rheumatism. In Malaysia, it is popularly known as 'Mata Ayam' and local traditional practitioners believed that the root of the plant is therapeutically beneficial.

METHODS

The cytotoxic effect of hydromethanolic extract of A. crispa and its solvents partitions (ethyl acetate and aqueous extracts) against breast cancer cells were evaluated by using MTT assay. The cells were treated with concentration of extracts ranging from 15.63 μg/mL- 1000 μg/mL for 72 h. The quantification of phenolic and flavonoid contents of the extracts were carried out to determine the relationship between of phytochemical compounds responsible for cytotoxic and antioxidative activities. The antioxidant capacity was measured by DPPH and ABTS free radical scavenging assay and expressed as milligram (mg) Trolox equivalent antioxidant capacity per 1 g (g) of tested extract.

RESULTS

The hydromethanolic and ethyl acetate extracts showed moderate cytotoxic effect against MCF-7 with IC50 values of 57.35 ± 19.33 μg/mL, and 54.98 ± 14.10 μg/mL, respectively but aqueous extract was inactive against MCF-7. For MDA-MB-231, hydromethanolic, ethyl acetate and aqueous extracts exhibited weak cytotoxic effects against MDA-MB-231 with IC50 values more than 100 μg/mL. The plant revealed high total phenolic content, total flavonoid and antioxidant capacity.

CONCLUSION

The response of different type of breast cancer cell lines towards A. crispa extract and its partitions varied. Accordingly, hydromethanolic and ethyl acetate extracts appear to be more cytotoxic to oestrogen receptor (ER) positive breast cancer than oestrogen receptor (ER) negative breast cancer. However, aqueous extract appears to have poor activity to both types of breast cancer. Besides that, hydromethanolic and ethyl acetate extracts exhibit higher TPC, TFC and antioxidant capacity compared to aqueous extract. Synergistic effect of anticancer and antioxidant bioactives compounds of A. crispa plausibly contributed to the cytotoxic effects of the extract.

Authors+Show Affiliations

Department of Clinical, Faculty of Veterinary Medicine, Universiti Malaysia Kelantan (UMK), Pengkalan Chepa, 16100, Kota Bharu, Kelantan, Malaysia. Department of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine, Universiti Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia.Department of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine, Universiti Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia. arifah@upm.edu.my.Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia.Department of Veterinary Laboratory Diagnostics, Faculty of Veterinary Medicine, Universiti Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia.Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29530022

Citation

Nordin, Muhammad Luqman, et al. "In Vitro Investigation of Cytotoxic and Antioxidative Activities of Ardisia Crispa Against Breast Cancer Cell Lines, MCF-7 and MDA-MB-231." BMC Complementary and Alternative Medicine, vol. 18, no. 1, 2018, p. 87.
Nordin ML, Abdul Kadir A, Zakaria ZA, et al. In vitro investigation of cytotoxic and antioxidative activities of Ardisia crispa against breast cancer cell lines, MCF-7 and MDA-MB-231. BMC Complement Altern Med. 2018;18(1):87.
Nordin, M. L., Abdul Kadir, A., Zakaria, Z. A., Abdullah, R., & Abdullah, M. N. H. (2018). In vitro investigation of cytotoxic and antioxidative activities of Ardisia crispa against breast cancer cell lines, MCF-7 and MDA-MB-231. BMC Complementary and Alternative Medicine, 18(1), 87. https://doi.org/10.1186/s12906-018-2153-5
Nordin ML, et al. In Vitro Investigation of Cytotoxic and Antioxidative Activities of Ardisia Crispa Against Breast Cancer Cell Lines, MCF-7 and MDA-MB-231. BMC Complement Altern Med. 2018 Mar 12;18(1):87. PubMed PMID: 29530022.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro investigation of cytotoxic and antioxidative activities of Ardisia crispa against breast cancer cell lines, MCF-7 and MDA-MB-231. AU - Nordin,Muhammad Luqman, AU - Abdul Kadir,Arifah, AU - Zakaria,Zainul Amiruddin, AU - Abdullah,Rasedee, AU - Abdullah,Muhammad Nazrul Hakim, Y1 - 2018/03/12/ PY - 2017/06/20/received PY - 2018/03/02/accepted PY - 2018/3/14/entrez PY - 2018/3/14/pubmed PY - 2018/4/13/medline KW - Antioxidant KW - Ardisia crispa KW - Breast cancer KW - Cytotoxic KW - Hydromethanolic SP - 87 EP - 87 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 18 IS - 1 N2 - BACKGROUND: Ardisia crispa Thunb. D.C is used mostly in some parts of the Asian region by traditional practitioners to treat certain diseases associated with oxidative stress and inflammation including cancer and rheumatism. In Malaysia, it is popularly known as 'Mata Ayam' and local traditional practitioners believed that the root of the plant is therapeutically beneficial. METHODS: The cytotoxic effect of hydromethanolic extract of A. crispa and its solvents partitions (ethyl acetate and aqueous extracts) against breast cancer cells were evaluated by using MTT assay. The cells were treated with concentration of extracts ranging from 15.63 μg/mL- 1000 μg/mL for 72 h. The quantification of phenolic and flavonoid contents of the extracts were carried out to determine the relationship between of phytochemical compounds responsible for cytotoxic and antioxidative activities. The antioxidant capacity was measured by DPPH and ABTS free radical scavenging assay and expressed as milligram (mg) Trolox equivalent antioxidant capacity per 1 g (g) of tested extract. RESULTS: The hydromethanolic and ethyl acetate extracts showed moderate cytotoxic effect against MCF-7 with IC50 values of 57.35 ± 19.33 μg/mL, and 54.98 ± 14.10 μg/mL, respectively but aqueous extract was inactive against MCF-7. For MDA-MB-231, hydromethanolic, ethyl acetate and aqueous extracts exhibited weak cytotoxic effects against MDA-MB-231 with IC50 values more than 100 μg/mL. The plant revealed high total phenolic content, total flavonoid and antioxidant capacity. CONCLUSION: The response of different type of breast cancer cell lines towards A. crispa extract and its partitions varied. Accordingly, hydromethanolic and ethyl acetate extracts appear to be more cytotoxic to oestrogen receptor (ER) positive breast cancer than oestrogen receptor (ER) negative breast cancer. However, aqueous extract appears to have poor activity to both types of breast cancer. Besides that, hydromethanolic and ethyl acetate extracts exhibit higher TPC, TFC and antioxidant capacity compared to aqueous extract. Synergistic effect of anticancer and antioxidant bioactives compounds of A. crispa plausibly contributed to the cytotoxic effects of the extract. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/29530022/In_vitro_investigation_of_cytotoxic_and_antioxidative_activities_of_Ardisia_crispa_against_breast_cancer_cell_lines_MCF_7_and_MDA_MB_231_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-018-2153-5 DB - PRIME DP - Unbound Medicine ER -