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Implementation of Precursor and Neutral Loss Scans on a Miniature Ion Trap Mass Spectrometer and Performance Comparison to a Benchtop Linear Ion Trap.
J Am Soc Mass Spectrom. 2018 07; 29(7):1355-1364.JA

Abstract

Implementation of orthogonal double resonance precursor and neutral loss scans on the Mini 12 miniature rectilinear ion trap mass spectrometer is described, and performance is compared to that of a commercial Thermo linear trap quadropole (LTQ) linear ion trap. The ac frequency scan version of the technique at constant rf voltage is used here because it is operationally much simpler to implement. Remarkably, the Mini 12 shows up to two orders of magnitude higher sensitivity compared to that of the LTQ. Resolution on the LTQ is better than unit at scan speeds of ~ 400 Th/s, whereas peak widths on the Mini 12, on average, range from 0.5 to 2.0 Th full width at half maximum and depend heavily on the precursor ion Mathieu q parameter as well as the pump down time that precedes the mass scan. Both sensitivity and resolution are maximized under higher pressure conditions (short pump down time) on the Mini 12. The effective mass range of the product ion ejection waveform was found to be 5.8 Th on the Mini 12 in the precursor ion scan mode vs. that of 3.9 Th on the LTQ. In the neutral loss scan mode, the product ion selectivity was between 8 and 11 Th on the Mini 12 and between 7 and 8 Th on the LTQ. The effects of nonlinear resonance lines on the Mini 12 were also explored. Graphical Abstract ᅟ.

Authors+Show Affiliations

Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA.Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA.Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA.Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA. cooks@purdue.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29536414

Citation

Snyder, Dalton T., et al. "Implementation of Precursor and Neutral Loss Scans On a Miniature Ion Trap Mass Spectrometer and Performance Comparison to a Benchtop Linear Ion Trap." Journal of the American Society for Mass Spectrometry, vol. 29, no. 7, 2018, pp. 1355-1364.
Snyder DT, Szalwinski LJ, Hilger R, et al. Implementation of Precursor and Neutral Loss Scans on a Miniature Ion Trap Mass Spectrometer and Performance Comparison to a Benchtop Linear Ion Trap. J Am Soc Mass Spectrom. 2018;29(7):1355-1364.
Snyder, D. T., Szalwinski, L. J., Hilger, R., & Cooks, R. G. (2018). Implementation of Precursor and Neutral Loss Scans on a Miniature Ion Trap Mass Spectrometer and Performance Comparison to a Benchtop Linear Ion Trap. Journal of the American Society for Mass Spectrometry, 29(7), 1355-1364. https://doi.org/10.1007/s13361-018-1922-1
Snyder DT, et al. Implementation of Precursor and Neutral Loss Scans On a Miniature Ion Trap Mass Spectrometer and Performance Comparison to a Benchtop Linear Ion Trap. J Am Soc Mass Spectrom. 2018;29(7):1355-1364. PubMed PMID: 29536414.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Implementation of Precursor and Neutral Loss Scans on a Miniature Ion Trap Mass Spectrometer and Performance Comparison to a Benchtop Linear Ion Trap. AU - Snyder,Dalton T, AU - Szalwinski,Lucas J, AU - Hilger,Ryan, AU - Cooks,R Graham, Y1 - 2018/03/13/ PY - 2017/11/03/received PY - 2018/01/13/accepted PY - 2018/01/08/revised PY - 2018/3/15/pubmed PY - 2018/3/15/medline PY - 2018/3/15/entrez KW - MS/MS KW - Miniature mass spectrometer KW - Neutral loss scan KW - Precursor ion scan KW - Tandem mass spectrometry SP - 1355 EP - 1364 JF - Journal of the American Society for Mass Spectrometry JO - J. Am. Soc. Mass Spectrom. VL - 29 IS - 7 N2 - Implementation of orthogonal double resonance precursor and neutral loss scans on the Mini 12 miniature rectilinear ion trap mass spectrometer is described, and performance is compared to that of a commercial Thermo linear trap quadropole (LTQ) linear ion trap. The ac frequency scan version of the technique at constant rf voltage is used here because it is operationally much simpler to implement. Remarkably, the Mini 12 shows up to two orders of magnitude higher sensitivity compared to that of the LTQ. Resolution on the LTQ is better than unit at scan speeds of ~ 400 Th/s, whereas peak widths on the Mini 12, on average, range from 0.5 to 2.0 Th full width at half maximum and depend heavily on the precursor ion Mathieu q parameter as well as the pump down time that precedes the mass scan. Both sensitivity and resolution are maximized under higher pressure conditions (short pump down time) on the Mini 12. The effective mass range of the product ion ejection waveform was found to be 5.8 Th on the Mini 12 in the precursor ion scan mode vs. that of 3.9 Th on the LTQ. In the neutral loss scan mode, the product ion selectivity was between 8 and 11 Th on the Mini 12 and between 7 and 8 Th on the LTQ. The effects of nonlinear resonance lines on the Mini 12 were also explored. Graphical Abstract ᅟ. SN - 1879-1123 UR - https://www.unboundmedicine.com/medline/citation/29536414/Implementation_of_Precursor_and_Neutral_Loss_Scans_on_a_Miniature_Ion_Trap_Mass_Spectrometer_and_Performance_Comparison_to_a_Benchtop_Linear_Ion_Trap_ DB - PRIME DP - Unbound Medicine ER -
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