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Identification and comparison of individual chromosomes of three accessions of Hordeum chilense, Hordeum vulgare, and Triticum aestivum by FISH.
Genome. 2018 Jun; 61(6):387-396.G

Abstract

Karyotypes of three accessions of Hordeum chilense (H1, H16, and H7), Hordeum vulgare, and Triticum aestivum were characterized by physical mapping of several repetitive sequences. A total of 14 repetitive sequences were used as probes for fluorescence in situ hybridization (FISH) with the aim of identifying inter- and intraspecies polymorphisms. The (AG)12 and 4P6 probes only produced hybridization signals in wheat, the BAC7 probe only hybridized to the centromeric region of H. vulgare, and the pSc119.2 probe hybridized to both wheat and H. chilense, but not to H. vulgare. The remaining repetitive sequences used in this study produced a hybridization signal in all the genotypes. Probes pAs1, pTa-535, pTa71, CCS1, and CRW were much conserved, showing no significant polymorphism among the genotypes studied. Probes GAA, (AAC)5, (CTA)5, HvT01, and pTa794 produced the most different hybridization pattern. We identified large polymorphisms in the three accessions of H. chilense studied, supporting the proposal of the existence of different groups inside species of H. chilense. The set of probes described in this work allowed the identification of every single chromosome in all three species, providing a complete cytogenetic karyotype of H. chilense, H. vulgare, and T. aestivum chromosomes, which could be useful in wheat and tritordeum breeding programs.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Rey MD
John Innes Centre, Crop Genetics Department, Norwich NR4 7UH, United Kingdom. John Innes Centre, Crop Genetics Department, Norwich NR4 7UH, United Kingdom.
Moore G
John Innes Centre, Crop Genetics Department, Norwich NR4 7UH, United Kingdom. John Innes Centre, Crop Genetics Department, Norwich NR4 7UH, United Kingdom.
Martín AC
John Innes Centre, Crop Genetics Department, Norwich NR4 7UH, United Kingdom. John Innes Centre, Crop Genetics Department, Norwich NR4 7UH, United Kingdom.

MeSH

Chromosomes, PlantConserved SequenceGenotypeHordeumKaryotypePolymorphism, GeneticRepetitive Sequences, Nucleic AcidTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29544080

Citation

Rey, María-Dolores, et al. "Identification and Comparison of Individual Chromosomes of Three Accessions of Hordeum Chilense, Hordeum Vulgare, and Triticum Aestivum By FISH." Genome, vol. 61, no. 6, 2018, pp. 387-396.
Rey MD, Moore G, Martín AC. Identification and comparison of individual chromosomes of three accessions of Hordeum chilense, Hordeum vulgare, and Triticum aestivum by FISH. Genome. 2018;61(6):387-396.
Rey, M. D., Moore, G., & Martín, A. C. (2018). Identification and comparison of individual chromosomes of three accessions of Hordeum chilense, Hordeum vulgare, and Triticum aestivum by FISH. Genome, 61(6), 387-396. https://doi.org/10.1139/gen-2018-0016
Rey MD, Moore G, Martín AC. Identification and Comparison of Individual Chromosomes of Three Accessions of Hordeum Chilense, Hordeum Vulgare, and Triticum Aestivum By FISH. Genome. 2018;61(6):387-396. PubMed PMID: 29544080.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and comparison of individual chromosomes of three accessions of Hordeum chilense, Hordeum vulgare, and Triticum aestivum by FISH. AU - Rey,María-Dolores, AU - Moore,Graham, AU - Martín,Azahara C, Y1 - 2018/03/15/ PY - 2018/3/16/pubmed PY - 2018/11/9/medline PY - 2018/3/16/entrez KW - FISH KW - Hordeum KW - Triticum aestivum KW - repetitive sequences KW - séquences répétées KW - tritordeum SP - 387 EP - 396 JF - Genome JO - Genome VL - 61 IS - 6 N2 - Karyotypes of three accessions of Hordeum chilense (H1, H16, and H7), Hordeum vulgare, and Triticum aestivum were characterized by physical mapping of several repetitive sequences. A total of 14 repetitive sequences were used as probes for fluorescence in situ hybridization (FISH) with the aim of identifying inter- and intraspecies polymorphisms. The (AG)12 and 4P6 probes only produced hybridization signals in wheat, the BAC7 probe only hybridized to the centromeric region of H. vulgare, and the pSc119.2 probe hybridized to both wheat and H. chilense, but not to H. vulgare. The remaining repetitive sequences used in this study produced a hybridization signal in all the genotypes. Probes pAs1, pTa-535, pTa71, CCS1, and CRW were much conserved, showing no significant polymorphism among the genotypes studied. Probes GAA, (AAC)5, (CTA)5, HvT01, and pTa794 produced the most different hybridization pattern. We identified large polymorphisms in the three accessions of H. chilense studied, supporting the proposal of the existence of different groups inside species of H. chilense. The set of probes described in this work allowed the identification of every single chromosome in all three species, providing a complete cytogenetic karyotype of H. chilense, H. vulgare, and T. aestivum chromosomes, which could be useful in wheat and tritordeum breeding programs. SN - 1480-3321 UR - https://www.unboundmedicine.com/medline/citation/29544080/Identification_and_comparison_of_individual_chromosomes_of_three_accessions_of_Hordeum_chilense_Hordeum_vulgare_and_Triticum_aestivum_by_FISH_ DB - PRIME DP - Unbound Medicine ER -