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Pitavastatin treatment induces neuroprotection through the BDNF-TrkB signalling pathway in cultured cerebral neurons after oxygen-glucose deprivation.
Neurol Res. 2018 May; 40(5):391-397.NR

Abstract

OBJECTIVES

Along with their lipid-lowering effect, statins have been reported to have neuroprotective function in both in vivo and in vitro models of neurodegenerative diseases. We conducted this study in order to uncover the he neuroprotective effect of the lipophilic statin pitavastatin (PTV) and investigate the underlying molecular mechanisms using primary cultured cerebral neurons exposed to oxygen-glucose deprivation (OGD).

METHODS

The primary cultured cerebral neurons were randomly assigned into four groups: the control group, the pitavastatin treatment group, the OGD group and the OGD + pitavastatin treatment group. The pitavastatin's concentration were set as follows: 1μM, 15μM, 30μM. After 3 hours OGD treatment, we use MTT method to assessment cell viability, immunofluorescence to observe neuron morphology and western blot method analysis the BDNF, TrkB.

RESULTS

PTV at concentrations of 1 μM and 15 μM elevated the survival rate of cortical neurons exposed to OGD, whereas 30 μM PTV did not show such an effect. Moreover, PTV promoted neuronal dendrite growth at concentrations of 1 μM and 15 μM. Increased expression levels of brain-derived neurotrophic factor (BDNF) and tropomyosin-related kinase B (TrkB) were observed in both of the following two scenarios: when neurons were treated with PTV for 48 hours and when PTV was added after the OGD procedure.

CONCLUSION

Pitavastatin treatment induces neuroprotection in cultured cerebral neurons after oxygen-glucose deprivation this neuroprotection induced by PTV involves the BDNF-TrkB signalling pathway.

Authors+Show Affiliations

a Department of Neurology , The First Affiliated Hospital of Zhengzhou University , Zhengzhou , China.a Department of Neurology , The First Affiliated Hospital of Zhengzhou University , Zhengzhou , China.a Department of Neurology , The First Affiliated Hospital of Zhengzhou University , Zhengzhou , China.c School of Information and Engineering , Zhengzhou University , Zhengzhou , China.a Department of Neurology , The First Affiliated Hospital of Zhengzhou University , Zhengzhou , China. b Institute of Clinical Medical Research , The First Affiliated Hospital of Zhengzhou University , Zhengzhou , China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29544396

Citation

Cui, Xiaoyan, et al. "Pitavastatin Treatment Induces Neuroprotection Through the BDNF-TrkB Signalling Pathway in Cultured Cerebral Neurons After Oxygen-glucose Deprivation." Neurological Research, vol. 40, no. 5, 2018, pp. 391-397.
Cui X, Fu Z, Wang M, et al. Pitavastatin treatment induces neuroprotection through the BDNF-TrkB signalling pathway in cultured cerebral neurons after oxygen-glucose deprivation. Neurol Res. 2018;40(5):391-397.
Cui, X., Fu, Z., Wang, M., Nan, X., & Zhang, B. (2018). Pitavastatin treatment induces neuroprotection through the BDNF-TrkB signalling pathway in cultured cerebral neurons after oxygen-glucose deprivation. Neurological Research, 40(5), 391-397. https://doi.org/10.1080/01616412.2018.1447318
Cui X, et al. Pitavastatin Treatment Induces Neuroprotection Through the BDNF-TrkB Signalling Pathway in Cultured Cerebral Neurons After Oxygen-glucose Deprivation. Neurol Res. 2018;40(5):391-397. PubMed PMID: 29544396.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pitavastatin treatment induces neuroprotection through the BDNF-TrkB signalling pathway in cultured cerebral neurons after oxygen-glucose deprivation. AU - Cui,Xiaoyan, AU - Fu,Zhenqiang, AU - Wang,Menghan, AU - Nan,Xiaofei, AU - Zhang,Boai, Y1 - 2018/03/16/ PY - 2018/3/17/pubmed PY - 2018/9/12/medline PY - 2018/3/17/entrez KW - BDNF-TrkB KW - Pitavastatin KW - cerebral cortex KW - neuroprotection KW - oxygen-glucose deprivation SP - 391 EP - 397 JF - Neurological research JO - Neurol. Res. VL - 40 IS - 5 N2 - OBJECTIVES: Along with their lipid-lowering effect, statins have been reported to have neuroprotective function in both in vivo and in vitro models of neurodegenerative diseases. We conducted this study in order to uncover the he neuroprotective effect of the lipophilic statin pitavastatin (PTV) and investigate the underlying molecular mechanisms using primary cultured cerebral neurons exposed to oxygen-glucose deprivation (OGD). METHODS: The primary cultured cerebral neurons were randomly assigned into four groups: the control group, the pitavastatin treatment group, the OGD group and the OGD + pitavastatin treatment group. The pitavastatin's concentration were set as follows: 1μM, 15μM, 30μM. After 3 hours OGD treatment, we use MTT method to assessment cell viability, immunofluorescence to observe neuron morphology and western blot method analysis the BDNF, TrkB. RESULTS: PTV at concentrations of 1 μM and 15 μM elevated the survival rate of cortical neurons exposed to OGD, whereas 30 μM PTV did not show such an effect. Moreover, PTV promoted neuronal dendrite growth at concentrations of 1 μM and 15 μM. Increased expression levels of brain-derived neurotrophic factor (BDNF) and tropomyosin-related kinase B (TrkB) were observed in both of the following two scenarios: when neurons were treated with PTV for 48 hours and when PTV was added after the OGD procedure. CONCLUSION: Pitavastatin treatment induces neuroprotection in cultured cerebral neurons after oxygen-glucose deprivation this neuroprotection induced by PTV involves the BDNF-TrkB signalling pathway. SN - 1743-1328 UR - https://www.unboundmedicine.com/medline/citation/29544396/Pitavastatin_treatment_induces_neuroprotection_through_the_BDNF_TrkB_signalling_pathway_in_cultured_cerebral_neurons_after_oxygen_glucose_deprivation_ L2 - http://www.tandfonline.com/doi/full/10.1080/01616412.2018.1447318 DB - PRIME DP - Unbound Medicine ER -