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Chemical characterization, pharmacological effects, and toxicity of an ethanol extract of Celtis pallida Torr. (Cannabaceae) aerial parts.
J Ethnopharmacol. 2018 Jun 12; 219:126-132.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Celtis pallida Torr (Cannabaceae) is employed as a folk medicine for the treatment of inflammation, pain, skin infections, and diarrhea, among other diseases.

AIM OF THE STUDY

The purpose of this work was to assess the chemical composition, the in vitro and in vivo toxicity, the antimicrobial, anti-inflammatory, antidiarrheal, antinociceptive, locomotor, and sedative effects of an ethanolic extract obtained from Celtis pallida aerial parts (CPE).

MATERIALS AND METHODS

The composition of CPE was carried out by GC-MS. The in vitro and in vivo toxic activity of CPE was estimated with the comet assay (10-1000 µg/ml) for 5 h in peripheral blood mononuclear cells, and the acute toxicity test (500-5000 mg/kg p.o.), for 14 days, respectively. The antimicrobial effect of CPE was evaluated using the minimum inhibitory concentration (MIC) assay, whereas the antidiarrheal activity (10-200 mg/kg p.o.) was calculated using the castor oil test. The antinociceptive effects of CPE (50-200 mg/kg p.o.) were estimated with the acetic acid and formalin tests, as well as the hot plate test. The sedative and locomotor activities of CPE (50-200 mg/kg p.o.) were assessed with the pentobarbital-induced sleeping time test and the rotarod test, respectively.

RESULTS

The main compound found in CPE was the triterpene ursolic acid (22% of the extract). CPE at concentrations of 100 µg/ml or higher induced genotoxicity in vitro and showed low in vivo toxicity (LD50 > 5000 mg/kg p.o.). Additionally, CPE lacked (MIC > 400 µg/ml) antimicrobial activity but exerts antinociceptive (ED50 = 12.5 ± 1.5 mg/kg) and antidiarrheal effects (ED50 = 2.8 mg/kg), without inducing sedative effects or altering the locomotor activity. The antinociceptive activity of CPE suggests the participation of adrenoceptors, as well as the nitric oxide/cyclic guanosine monophosphate (cGMP) pathway.

CONCLUSION

C. pallida exerts its antinociceptive effects probably mediated by the nitric oxide/cyclic guanosine monophosphate (cGMP) pathway.

Authors+Show Affiliations

Department of Pharmacy, Division of Natural and Exact Sciences, University Guanajuato, Noria Alta sin número, C.P. 36040 Guanajuato, Gto., Mexico.Department of Pharmacy, Division of Natural and Exact Sciences, University Guanajuato, Noria Alta sin número, C.P. 36040 Guanajuato, Gto., Mexico.Department of Pharmacy, Division of Natural and Exact Sciences, University Guanajuato, Noria Alta sin número, C.P. 36040 Guanajuato, Gto., Mexico.School of Chemical Sciences, Autonomous University of San Luis Potosí, San Luis Potosí, Mexico.Department of Pharmacy, Division of Natural and Exact Sciences, University Guanajuato, Noria Alta sin número, C.P. 36040 Guanajuato, Gto., Mexico.Department of Pharmacy, Division of Natural and Exact Sciences, University Guanajuato, Noria Alta sin número, C.P. 36040 Guanajuato, Gto., Mexico.Multidisciplinary Academic Unit, Huastec Region, Autonomous University of San Luis Potosí, Ciudad Valles, San Luis Potosí, Mexico.Multidisciplinary Academic Unit, Huastec Region, Autonomous University of San Luis Potosí, Ciudad Valles, San Luis Potosí, Mexico.Department of Pharmacy, Division of Natural and Exact Sciences, University Guanajuato, Noria Alta sin número, C.P. 36040 Guanajuato, Gto., Mexico.Department of Pharmacy, Division of Natural and Exact Sciences, University Guanajuato, Noria Alta sin número, C.P. 36040 Guanajuato, Gto., Mexico. Electronic address: angeljosabad@ugto.mx.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29545209

Citation

Rojas-Bedolla, Edgar Isaac, et al. "Chemical Characterization, Pharmacological Effects, and Toxicity of an Ethanol Extract of Celtis Pallida Torr. (Cannabaceae) Aerial Parts." Journal of Ethnopharmacology, vol. 219, 2018, pp. 126-132.
Rojas-Bedolla EI, Gutiérrez-Pérez JL, Arenas-López MI, et al. Chemical characterization, pharmacological effects, and toxicity of an ethanol extract of Celtis pallida Torr. (Cannabaceae) aerial parts. J Ethnopharmacol. 2018;219:126-132.
Rojas-Bedolla, E. I., Gutiérrez-Pérez, J. L., Arenas-López, M. I., González-Chávez, M. M., Zapata-Morales, J. R., Mendoza-Macías, C. L., Carranza-Álvarez, C., Maldonado-Miranda, J. J., Deveze-Álvarez, M. A., & Alonso-Castro, A. J. (2018). Chemical characterization, pharmacological effects, and toxicity of an ethanol extract of Celtis pallida Torr. (Cannabaceae) aerial parts. Journal of Ethnopharmacology, 219, 126-132. https://doi.org/10.1016/j.jep.2018.03.014
Rojas-Bedolla EI, et al. Chemical Characterization, Pharmacological Effects, and Toxicity of an Ethanol Extract of Celtis Pallida Torr. (Cannabaceae) Aerial Parts. J Ethnopharmacol. 2018 Jun 12;219:126-132. PubMed PMID: 29545209.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemical characterization, pharmacological effects, and toxicity of an ethanol extract of Celtis pallida Torr. (Cannabaceae) aerial parts. AU - Rojas-Bedolla,Edgar Isaac, AU - Gutiérrez-Pérez,Jorge Luis, AU - Arenas-López,Mario Iván, AU - González-Chávez,Marco Martin, AU - Zapata-Morales,Juan Ramón, AU - Mendoza-Macías,Claudia Leticia, AU - Carranza-Álvarez,Candy, AU - Maldonado-Miranda,Juan José, AU - Deveze-Álvarez,Martha Alicia, AU - Alonso-Castro,Angel Josabad, Y1 - 2018/03/12/ PY - 2017/07/03/received PY - 2018/02/03/revised PY - 2018/03/09/accepted PY - 2018/3/17/pubmed PY - 2018/10/3/medline PY - 2018/3/17/entrez KW - Antimicrobial KW - Carbamoyl choline (PubChem CID:5831) KW - Celtis pallida KW - Clonazepam (PubChem CID:2802) KW - Diarrhea KW - Dimethyl sulfoxide (PubChem CID:679) KW - Glibenclamide (PubChem CID:3488) KW - Inflammation KW - Naloxone (PubChem CID:5464092) KW - Naproxen sodium (PubChem CID: 23681059) KW - Pain KW - Tramadol hydrochloride (PubChem CID:63013) KW - pentobarbital (PubChem CID: 4737) SP - 126 EP - 132 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 219 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Celtis pallida Torr (Cannabaceae) is employed as a folk medicine for the treatment of inflammation, pain, skin infections, and diarrhea, among other diseases. AIM OF THE STUDY: The purpose of this work was to assess the chemical composition, the in vitro and in vivo toxicity, the antimicrobial, anti-inflammatory, antidiarrheal, antinociceptive, locomotor, and sedative effects of an ethanolic extract obtained from Celtis pallida aerial parts (CPE). MATERIALS AND METHODS: The composition of CPE was carried out by GC-MS. The in vitro and in vivo toxic activity of CPE was estimated with the comet assay (10-1000 µg/ml) for 5 h in peripheral blood mononuclear cells, and the acute toxicity test (500-5000 mg/kg p.o.), for 14 days, respectively. The antimicrobial effect of CPE was evaluated using the minimum inhibitory concentration (MIC) assay, whereas the antidiarrheal activity (10-200 mg/kg p.o.) was calculated using the castor oil test. The antinociceptive effects of CPE (50-200 mg/kg p.o.) were estimated with the acetic acid and formalin tests, as well as the hot plate test. The sedative and locomotor activities of CPE (50-200 mg/kg p.o.) were assessed with the pentobarbital-induced sleeping time test and the rotarod test, respectively. RESULTS: The main compound found in CPE was the triterpene ursolic acid (22% of the extract). CPE at concentrations of 100 µg/ml or higher induced genotoxicity in vitro and showed low in vivo toxicity (LD50 > 5000 mg/kg p.o.). Additionally, CPE lacked (MIC > 400 µg/ml) antimicrobial activity but exerts antinociceptive (ED50 = 12.5 ± 1.5 mg/kg) and antidiarrheal effects (ED50 = 2.8 mg/kg), without inducing sedative effects or altering the locomotor activity. The antinociceptive activity of CPE suggests the participation of adrenoceptors, as well as the nitric oxide/cyclic guanosine monophosphate (cGMP) pathway. CONCLUSION: C. pallida exerts its antinociceptive effects probably mediated by the nitric oxide/cyclic guanosine monophosphate (cGMP) pathway. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/29545209/Chemical_characterization_pharmacological_effects_and_toxicity_of_an_ethanol_extract_of_Celtis_pallida_Torr___Cannabaceae__aerial_parts_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(17)32511-4 DB - PRIME DP - Unbound Medicine ER -