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Influence of different peritoneal dialysis fluids on the in vitro activity of fosfomycin against Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa.
Eur J Clin Microbiol Infect Dis. 2018 Jun; 37(6):1091-1098.EJ

Abstract

Peritonitis is still the main infectious complication among patients on peritoneal dialysis. For treatment of peritoneal dialysis-related peritonitis, the intraperitoneal administration of antibiotics admixed to peritoneal dialysis fluids (PDFs) should be preferred. However, the influence of diverse PDFs on the activity of frequently used antibiotics has been investigated insufficiently. Thus, the present study set out to investigate the in vitro activity of fosfomycin against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermidis, and Staphylococcus aureus in commercially available PDFs. Time-kill curves in four different PDFs (Dianeal®, Extraneal®, Nutrineal®, and Physioneal®) were performed over 24 h with two different concentrations of fosfomycin (150 and 400 mg/L) and without antibiotics as control. Cation-adjusted Mueller Hinton broth (CA-MHB) was used as a comparator solution. In blank PDFs, bacterial growth of each organism evaluated was reduced when compared to CA-MHB. For S. aureus in blank Physioneal®, a reduction under the limit of detection was observed within 24 h. The activity of fosfomycin was reduced in all PDFs when compared to CA-MHB except for P. aeruginosa in Nutrineal® where the activity of fosfomycin was increased when investigated at 400 mg/L. Against E.coli, bactericidal activity was demonstrated in Extraneal®, Nutrineal®, and Physioneal®. Fosfomycin resistance (MIC > 1024 mg/L) was observed for P. aeruginosa in CA-MHB at both concentrations and in Nutrineal® at 150 mg/L. Fosfomycin is active in PDFs particularly against the frequently isolated enterobacterium E. coli. The choice of the respective PDF considerably influences the microbiological outcome in vitro. Further studies are warranted to investigate the clinical relevance of these findings.

Authors+Show Affiliations

Department of Internal Medicine I, Division of Infectious Diseases and Tropical Medicine, Medical University Vienna, Vienna, Austria.Department of Internal Medicine I, Division of Infectious Diseases and Tropical Medicine, Medical University Vienna, Vienna, Austria.Department of Internal Medicine I, University hospital St. Poelten, Karl Landsteiner University of Health Sciences, St. Poelten, Austria.Department of Pharmacognosy, University of Vienna, Vienna, Austria.Department of Internal Medicine I, Division of Infectious Diseases and Tropical Medicine, Medical University Vienna, Vienna, Austria.Department of Internal Medicine I, Division of Infectious Diseases and Tropical Medicine, Medical University Vienna, Vienna, Austria. Military Medical Cluster East, Austrian Armed Forces, Vienna, Austria.Department of Clinical Pharmacology, Medical University Vienna, Waehringerguertel 18-20, A-1090, Vienna, Austria. markus.zeitlinger@meduniwien.ac.at.Department of Internal Medicine I, University hospital St. Poelten, Karl Landsteiner University of Health Sciences, St. Poelten, Austria.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29546637

Citation

Kussmann, Manuel, et al. "Influence of Different Peritoneal Dialysis Fluids On the in Vitro Activity of Fosfomycin Against Escherichia Coli, Staphylococcus Aureus, Staphylococcus Epidermidis, and Pseudomonas Aeruginosa." European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology, vol. 37, no. 6, 2018, pp. 1091-1098.
Kussmann M, Hauer S, Pichler P, et al. Influence of different peritoneal dialysis fluids on the in vitro activity of fosfomycin against Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. Eur J Clin Microbiol Infect Dis. 2018;37(6):1091-1098.
Kussmann, M., Hauer, S., Pichler, P., Reznicek, G., Burgmann, H., Poeppl, W., Zeitlinger, M., & Wiesholzer, M. (2018). Influence of different peritoneal dialysis fluids on the in vitro activity of fosfomycin against Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology, 37(6), 1091-1098. https://doi.org/10.1007/s10096-018-3221-y
Kussmann M, et al. Influence of Different Peritoneal Dialysis Fluids On the in Vitro Activity of Fosfomycin Against Escherichia Coli, Staphylococcus Aureus, Staphylococcus Epidermidis, and Pseudomonas Aeruginosa. Eur J Clin Microbiol Infect Dis. 2018;37(6):1091-1098. PubMed PMID: 29546637.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of different peritoneal dialysis fluids on the in vitro activity of fosfomycin against Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. AU - Kussmann,Manuel, AU - Hauer,Stefan, AU - Pichler,Petra, AU - Reznicek,Gottfried, AU - Burgmann,Heinz, AU - Poeppl,Wolfgang, AU - Zeitlinger,Markus, AU - Wiesholzer,Martin, Y1 - 2018/03/15/ PY - 2018/02/01/received PY - 2018/02/25/accepted PY - 2018/3/17/pubmed PY - 2018/11/20/medline PY - 2018/3/17/entrez KW - Antibiotics KW - Infection KW - Peritoneal dialysis solutions KW - Peritonitis KW - Time-kill curves SP - 1091 EP - 1098 JF - European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology JO - Eur. J. Clin. Microbiol. Infect. Dis. VL - 37 IS - 6 N2 - Peritonitis is still the main infectious complication among patients on peritoneal dialysis. For treatment of peritoneal dialysis-related peritonitis, the intraperitoneal administration of antibiotics admixed to peritoneal dialysis fluids (PDFs) should be preferred. However, the influence of diverse PDFs on the activity of frequently used antibiotics has been investigated insufficiently. Thus, the present study set out to investigate the in vitro activity of fosfomycin against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermidis, and Staphylococcus aureus in commercially available PDFs. Time-kill curves in four different PDFs (Dianeal®, Extraneal®, Nutrineal®, and Physioneal®) were performed over 24 h with two different concentrations of fosfomycin (150 and 400 mg/L) and without antibiotics as control. Cation-adjusted Mueller Hinton broth (CA-MHB) was used as a comparator solution. In blank PDFs, bacterial growth of each organism evaluated was reduced when compared to CA-MHB. For S. aureus in blank Physioneal®, a reduction under the limit of detection was observed within 24 h. The activity of fosfomycin was reduced in all PDFs when compared to CA-MHB except for P. aeruginosa in Nutrineal® where the activity of fosfomycin was increased when investigated at 400 mg/L. Against E.coli, bactericidal activity was demonstrated in Extraneal®, Nutrineal®, and Physioneal®. Fosfomycin resistance (MIC > 1024 mg/L) was observed for P. aeruginosa in CA-MHB at both concentrations and in Nutrineal® at 150 mg/L. Fosfomycin is active in PDFs particularly against the frequently isolated enterobacterium E. coli. The choice of the respective PDF considerably influences the microbiological outcome in vitro. Further studies are warranted to investigate the clinical relevance of these findings. SN - 1435-4373 UR - https://www.unboundmedicine.com/medline/citation/29546637/Influence_of_different_peritoneal_dialysis_fluids_on_the_in_vitro_activity_of_fosfomycin_against_Escherichia_coli_Staphylococcus_aureus_Staphylococcus_epidermidis_and_Pseudomonas_aeruginosa_ L2 - https://dx.doi.org/10.1007/s10096-018-3221-y DB - PRIME DP - Unbound Medicine ER -