Tags

Type your tag names separated by a space and hit enter

A Novel Betabaculovirus Isolated from the Monocot Pest Mocis latipes (Lepidoptera: Noctuidae) and the Evolution of Multiple-Copy Genes.
Viruses 2018; 10(3)V

Abstract

In this report, we described the genome of a novel baculovirus isolated from the monocot insect pest Mocis latipes, the striped grass looper. The genome has 134,272 bp in length with a G + C content of 38.3%. Based on the concatenated sequence of the 38 baculovirus core genes, we found that the virus is a betabaculovirus closely related to the noctuid-infecting betabaculoviruses including Pseudaletia unipuncta granulovirus (PsunGV), Trichoplusia ni granulovirus (TnGV), Helicoverpa armigera granulovirus (HearGV), and Xestia c-nigrum granulovirus (XecnGV). The virus may constitute a new Betabaculovirus species tentatively named Mocis latipes granulovirus (MolaGV). After gene content analysis, five open reading frames (ORFs) were found to be unique to MolaGV and several auxiliary genes were found including iap-3, iap-5, bro-a, bro-b, and three enhancins. The virus genome lacked both chitinase and cathepsin. We then looked at the evolutionary history of the enhancin gene and found that betabaculovirus acquired this gene from an alphabaculovirus followed by several duplication events. Gene duplication also happened to an endonuclease-like gene. Genomic and gene content analyses revealed both a strict collinearity and gene expansion into the genome of the MolaGV-related species. We also characterized the granulin gene using a recombinant Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and found that occlusion bodies were produced into the nucleus of infected cells and presented a polyhedral shape and no occluded virions within. Overall, betabaculovirus genome sequencing is of importance to the field as few genomes are publicly accessible. Mocislatipes is a secondary pest of maize, rice, and wheat crops in Brazil. Certainly, both the discovery and description of novel baculoviruses may lead to development of greener and safer pesticides in order to counteract and effectively control crop damage-causing insect populations.

Authors+Show Affiliations

Laboratory of Insect Virology, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria, Santa Maria RS 97105-900, Brazil. daniel_ardisson@yahoo.com.br.Laboratory of Baculovirus, Cell Biology Department, University of Brasilia, Brasília DF 70910-900, Brazil. ethi_rozo@hotmail.com.Laboratory of Baculovirus, Cell Biology Department, University of Brasilia, Brasília DF 70910-900, Brazil. flucasmelo@gmail.com.Laboratory of Insect Virology, Department of Biochemistry and Molecular Biology, Federal University of Santa Maria, Santa Maria RS 97105-900, Brazil. anmag2001@yahoo.com.br.Embrapa-Soja, Distrito de Warta P.O. Box 231, Londrina PR 86001-970, Brazil. daniel.sosa-gomez@embrapa.br.Laboratory of Baculovirus, Cell Biology Department, University of Brasilia, Brasília DF 70910-900, Brazil. bergmann@unb.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29547534

Citation

Ardisson-Araújo, Daniel M P., et al. "A Novel Betabaculovirus Isolated From the Monocot Pest Mocis Latipes (Lepidoptera: Noctuidae) and the Evolution of Multiple-Copy Genes." Viruses, vol. 10, no. 3, 2018.
Ardisson-Araújo DMP, da Silva AMR, Melo FL, et al. A Novel Betabaculovirus Isolated from the Monocot Pest Mocis latipes (Lepidoptera: Noctuidae) and the Evolution of Multiple-Copy Genes. Viruses. 2018;10(3).
Ardisson-Araújo, D. M. P., da Silva, A. M. R., Melo, F. L., Dos Santos, E. R., Sosa-Gómez, D. R., & Ribeiro, B. M. (2018). A Novel Betabaculovirus Isolated from the Monocot Pest Mocis latipes (Lepidoptera: Noctuidae) and the Evolution of Multiple-Copy Genes. Viruses, 10(3), doi:10.3390/v10030134.
Ardisson-Araújo DMP, et al. A Novel Betabaculovirus Isolated From the Monocot Pest Mocis Latipes (Lepidoptera: Noctuidae) and the Evolution of Multiple-Copy Genes. Viruses. 2018 03 16;10(3) PubMed PMID: 29547534.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Novel Betabaculovirus Isolated from the Monocot Pest Mocis latipes (Lepidoptera: Noctuidae) and the Evolution of Multiple-Copy Genes. AU - Ardisson-Araújo,Daniel M P, AU - da Silva,Ana Maria Rodrigues, AU - Melo,Fernando L, AU - Dos Santos,Ethiane Rozo, AU - Sosa-Gómez,Daniel R, AU - Ribeiro,Bergmann M, Y1 - 2018/03/16/ PY - 2017/12/18/received PY - 2018/03/12/revised PY - 2018/03/14/accepted PY - 2018/3/17/entrez PY - 2018/3/17/pubmed PY - 2018/9/19/medline KW - Mocis latipes granulovirus KW - baculovirus genome KW - betabaculovirus KW - endonuclease-like KW - enhancin KW - evolution KW - genome expansion KW - granulin JF - Viruses JO - Viruses VL - 10 IS - 3 N2 - In this report, we described the genome of a novel baculovirus isolated from the monocot insect pest Mocis latipes, the striped grass looper. The genome has 134,272 bp in length with a G + C content of 38.3%. Based on the concatenated sequence of the 38 baculovirus core genes, we found that the virus is a betabaculovirus closely related to the noctuid-infecting betabaculoviruses including Pseudaletia unipuncta granulovirus (PsunGV), Trichoplusia ni granulovirus (TnGV), Helicoverpa armigera granulovirus (HearGV), and Xestia c-nigrum granulovirus (XecnGV). The virus may constitute a new Betabaculovirus species tentatively named Mocis latipes granulovirus (MolaGV). After gene content analysis, five open reading frames (ORFs) were found to be unique to MolaGV and several auxiliary genes were found including iap-3, iap-5, bro-a, bro-b, and three enhancins. The virus genome lacked both chitinase and cathepsin. We then looked at the evolutionary history of the enhancin gene and found that betabaculovirus acquired this gene from an alphabaculovirus followed by several duplication events. Gene duplication also happened to an endonuclease-like gene. Genomic and gene content analyses revealed both a strict collinearity and gene expansion into the genome of the MolaGV-related species. We also characterized the granulin gene using a recombinant Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and found that occlusion bodies were produced into the nucleus of infected cells and presented a polyhedral shape and no occluded virions within. Overall, betabaculovirus genome sequencing is of importance to the field as few genomes are publicly accessible. Mocislatipes is a secondary pest of maize, rice, and wheat crops in Brazil. Certainly, both the discovery and description of novel baculoviruses may lead to development of greener and safer pesticides in order to counteract and effectively control crop damage-causing insect populations. SN - 1999-4915 UR - https://www.unboundmedicine.com/medline/citation/29547534/A_Novel_Betabaculovirus_Isolated_from_the_Monocot_Pest_Mocis_latipes__Lepidoptera:_Noctuidae__and_the_Evolution_of_Multiple_Copy_Genes_ L2 - http://www.mdpi.com/resolver?pii=v10030134 DB - PRIME DP - Unbound Medicine ER -