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Specificity in Arabidopsis thaliana recruitment of root fungal communities from soil and rhizosphere.
Fungal Biol. 2018 04; 122(4):231-240.FB

Abstract

Biotic and abiotic conditions in soil pose major constraints on growth and reproductive success of plants. Fungi are important agents in plant soil interactions but the belowground mycobiota associated with plants remains poorly understood. We grew one genotype each from Sweden and Italy of the widely-studied plant model Arabidopsis thaliana. Plants were grown under controlled conditions in organic topsoil local to the Swedish genotype, and harvested after ten weeks. Total DNA was extracted from three belowground compartments: endosphere (sonicated roots), rhizosphere and bulk soil, and fungal communities were characterized from each by amplification and sequencing of the fungal barcode region ITS2. Fungal species diversity was found to decrease from bulk soil to rhizosphere to endosphere. A significant effect of plant genotype on fungal community composition was detected only in the endosphere compartment. Despite A. thaliana being a non-mycorrhizal plant, it hosts a number of known mycorrhiza fungi in its endosphere compartment, which is also colonized by endophytic, pathogenic and saprotrophic fungi. Species in the Archaeorhizomycetes were most abundant in rhizosphere samples suggesting an adaptation to environments with high nutrient turnover for some of these species. We conclude that A. thaliana endosphere fungal communities represent a selected subset of fungi recruited from soil and that plant genotype has small but significant quantitative and qualitative effects on these communities.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Urbina H
Department of Ecology and Genetics, Uppsala University, Norbyvägen 18D, SE-75236, Uppsala, Sweden; Department of Botany and Plant Pathology, Purdue University, 915 W State St, West Lafayette, IN, 47907, USA.
Breed MF
Department of Ecology and Genetics, Uppsala University, Norbyvägen 18D, SE-75236, Uppsala, Sweden; School of Biological Sciences and the Environment Institute, University of Adelaide, North Terrace, SA-5005, Australia.
Zhao W
Department of Molecular Evolution, Cell and Molecular Biology, Uppsala University, Husargatan 3, SE-75124, Uppsala, Sweden.
Lakshmi Gurrala K
Department of Molecular Evolution, Cell and Molecular Biology, Uppsala University, Husargatan 3, SE-75124, Uppsala, Sweden.
Andersson SGE
Department of Molecular Evolution, Cell and Molecular Biology, Uppsala University, Husargatan 3, SE-75124, Uppsala, Sweden.
Ågren J
Department of Ecology and Genetics, Uppsala University, Norbyvägen 18D, SE-75236, Uppsala, Sweden.
Baldauf S
Department of Organismal Biology, Uppsala University, Norbyvägen 18D, SE-75236, Uppsala, Sweden.
Rosling A
Department of Ecology and Genetics, Uppsala University, Norbyvägen 18D, SE-75236, Uppsala, Sweden. Electronic address: Anna.Rosling@ebc.uu.se.

MeSH

ArabidopsisCluster AnalysisDNA, FungalDNA, Ribosomal SpacerFungiItalyMycobiomePhylogenyPlant RootsRhizosphereSequence Analysis, DNASoil MicrobiologySweden

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29551197

Citation

Urbina, Hector, et al. "Specificity in Arabidopsis Thaliana Recruitment of Root Fungal Communities From Soil and Rhizosphere." Fungal Biology, vol. 122, no. 4, 2018, pp. 231-240.
Urbina H, Breed MF, Zhao W, et al. Specificity in Arabidopsis thaliana recruitment of root fungal communities from soil and rhizosphere. Fungal Biol. 2018;122(4):231-240.
Urbina, H., Breed, M. F., Zhao, W., Lakshmi Gurrala, K., Andersson, S. G. E., Ågren, J., Baldauf, S., & Rosling, A. (2018). Specificity in Arabidopsis thaliana recruitment of root fungal communities from soil and rhizosphere. Fungal Biology, 122(4), 231-240. https://doi.org/10.1016/j.funbio.2017.12.013
Urbina H, et al. Specificity in Arabidopsis Thaliana Recruitment of Root Fungal Communities From Soil and Rhizosphere. Fungal Biol. 2018;122(4):231-240. PubMed PMID: 29551197.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Specificity in Arabidopsis thaliana recruitment of root fungal communities from soil and rhizosphere. AU - Urbina,Hector, AU - Breed,Martin F, AU - Zhao,Weizhou, AU - Lakshmi Gurrala,Kanaka, AU - Andersson,Siv G E, AU - Ågren,Jon, AU - Baldauf,Sandra, AU - Rosling,Anna, Y1 - 2018/01/10/ PY - 2017/11/30/received PY - 2017/12/23/accepted PY - 2018/3/20/entrez PY - 2018/3/20/pubmed PY - 2019/1/29/medline KW - Arabidopsis KW - Archaeorhizomcyetes KW - Brassicaceae KW - ITS metabarcoding KW - Ion Torrent KW - Rhizosphere SP - 231 EP - 240 JF - Fungal biology JO - Fungal Biol VL - 122 IS - 4 N2 - Biotic and abiotic conditions in soil pose major constraints on growth and reproductive success of plants. Fungi are important agents in plant soil interactions but the belowground mycobiota associated with plants remains poorly understood. We grew one genotype each from Sweden and Italy of the widely-studied plant model Arabidopsis thaliana. Plants were grown under controlled conditions in organic topsoil local to the Swedish genotype, and harvested after ten weeks. Total DNA was extracted from three belowground compartments: endosphere (sonicated roots), rhizosphere and bulk soil, and fungal communities were characterized from each by amplification and sequencing of the fungal barcode region ITS2. Fungal species diversity was found to decrease from bulk soil to rhizosphere to endosphere. A significant effect of plant genotype on fungal community composition was detected only in the endosphere compartment. Despite A. thaliana being a non-mycorrhizal plant, it hosts a number of known mycorrhiza fungi in its endosphere compartment, which is also colonized by endophytic, pathogenic and saprotrophic fungi. Species in the Archaeorhizomycetes were most abundant in rhizosphere samples suggesting an adaptation to environments with high nutrient turnover for some of these species. We conclude that A. thaliana endosphere fungal communities represent a selected subset of fungi recruited from soil and that plant genotype has small but significant quantitative and qualitative effects on these communities. SN - 1878-6146 UR - https://www.unboundmedicine.com/medline/citation/29551197/Specificity_in_Arabidopsis_thaliana_recruitment_of_root_fungal_communities_from_soil_and_rhizosphere_ DB - PRIME DP - Unbound Medicine ER -