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[Electroacupuncture plus Gastrodin Improves Learning-memory Ability Possibly by Up-regulating Expression of SIRT 1 and PGC-1 ɑ in Hippocampal CA 1 Region of Alzheimer's Disease Rats].
Zhen Ci Yan Jiu. 2018 Mar 25; 43(3):140-5.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) combined with Gastrodin on learning-memory ability and expression of silent information regulator 2 homologous protein 1(SIRT 1) and peroxisome proliferator activated receptor γ coactivator (PGC-1 ɑ) of hippocampal CA 1 region in Alzheimer's disease(AD) rats, so as to explore its mechanism under-lying improvement of AD.

METHODS

Sixty male SD rats were randomly divided into normal control (normal), sham operation (sham), model, EA, Gastrodin and EA+ Gastrodin groups (n=10 in each). The AD model was established by intraperitoneal injection of D-Galactose (120 mg•kg-1•d-1) combined with bilateral hippocampal injection of β amyloid 1-40(Aβ 1-40). EA was applied at "Baihui"(GV 20), "Dazhui"(GV 14) and "Zusanli"(ST 36) for 30 min, once daily for 4 weeks. For rats of the Gastro-din group and EA+ Gastrodin group, intraperitoneal injection of gastrodin(10 mg/kg) was conducted once daily for 4 weeks. Morris water maze tests were used to assess the rat's learning-memory ability. Nissl staining was used to assess the morphological changes of neurons in the hippocampal CA 1 area. The expression of SIRT 1 and PGC-1 ɑ of hippocampal CA 1 region was mea-sured by immunohistochemical staining.

RESULTS

1) Morris water maze tests showed that, compared with the normal and sham group, the escape latency was significantly prolonged (P<0.05), and the percentage of platform quadrant residence duration and the platform crossing times were considerably decreased in the model group (P<0.05). After the intervention, the escape latency was obviously shortened (P<0.05), and the percentage of platform quadrant residence duration and the platform crossing times were markedly increased in the EA, Gastrodin and EA+Gastrodin groups relevant to the model group (P<0.05). 2) Nissl staining showed that, in comparison with the normal group or sham group, the number of cells in the hippocampal CA 1 area was decreased and the arrangement was disorganized in the model group. The number of cells in CA 1 area was relatively higher in the 3 treatment groups than in the model group. 3) The expression levels of SIRT 1 and PGC-1 ɑ proteins in the hippocampal CA 1 area were significantly down-regulated in the model group than in the normal and sham groups (P<0.05). After the intervention, the expression levels of SIRT 1 and PGC-1 ɑ in the EA, Gastrodin and EA+Gastrodin groups were significantly up-regulated compared with the model group (P<0.05). The effects of EA+Gastrodin were significantly superior to those of simple EA and simple Gastrodin in shortening the escape latency, up-regulating the expression levels of SIRT 1 and PGC-1 ɑ as well as in increasing the percentage of platform quadrant residence time and platform crossing times (P<0.05).

CONCLUSION

Both EA and Gastrodin can improve the learning-memory ability of AD rats, which may be related to their effects in up-regulating the expression of SIRT 1 and PGC-1 ɑ and reducing neuronal injury in the CA 1 region of hippocampus, suggesting a protective role of EA on hippocampal neurons. The effect of EA combined with Gastrodin is markedly better than that of EA and Gastrodin alone.

Authors+Show Affiliations

Department of Anatomy, Wannan Medical College, Wuhu 241002, Anhui Province, China.Department of Anatomy, Wannan Medical College, Wuhu 241002, Anhui Province, China.Department of Anatomy, Wannan Medical College, Wuhu 241002, Anhui Province, China.Department of Anatomy, Wannan Medical College, Wuhu 241002, Anhui Province, China.Department of Anatomy, Wannan Medical College, Wuhu 241002, Anhui Province, China.Department of Anatomy, Wannan Medical College, Wuhu 241002, Anhui Province, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

29560628

Citation

Huang, Rui, et al. "[Electroacupuncture Plus Gastrodin Improves Learning-memory Ability Possibly By Up-regulating Expression of SIRT 1 and PGC-1 Ɑ in Hippocampal CA 1 Region of Alzheimer's Disease Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 43, no. 3, 2018, pp. 140-5.
Huang R, Wu F, Zhao J, et al. [Electroacupuncture plus Gastrodin Improves Learning-memory Ability Possibly by Up-regulating Expression of SIRT 1 and PGC-1 ɑ in Hippocampal CA 1 Region of Alzheimer's Disease Rats]. Zhen Ci Yan Jiu. 2018;43(3):140-5.
Huang, R., Wu, F., Zhao, J., Li, H. B., Ding, J., & Xiong, K. R. (2018). [Electroacupuncture plus Gastrodin Improves Learning-memory Ability Possibly by Up-regulating Expression of SIRT 1 and PGC-1 ɑ in Hippocampal CA 1 Region of Alzheimer's Disease Rats]. Zhen Ci Yan Jiu = Acupuncture Research, 43(3), 140-5. https://doi.org/10.13702/j.1000-0607.170635
Huang R, et al. [Electroacupuncture Plus Gastrodin Improves Learning-memory Ability Possibly By Up-regulating Expression of SIRT 1 and PGC-1 Ɑ in Hippocampal CA 1 Region of Alzheimer's Disease Rats]. Zhen Ci Yan Jiu. 2018 Mar 25;43(3):140-5. PubMed PMID: 29560628.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Electroacupuncture plus Gastrodin Improves Learning-memory Ability Possibly by Up-regulating Expression of SIRT 1 and PGC-1 ɑ in Hippocampal CA 1 Region of Alzheimer's Disease Rats]. AU - Huang,Rui, AU - Wu,Feng, AU - Zhao,Jian, AU - Li,Huai-Bin, AU - Ding,Jian, AU - Xiong,Ke-Ren, PY - 2018/3/22/entrez PY - 2018/3/22/pubmed PY - 2019/4/6/medline KW - Alzheimer’s disease KW - Electroacupuncture KW - Gastrodin KW - Hippocampal CA 1 KW - Learning-memory ability KW - Peroxisome proliferator activated receptor γ coactivator KW - Silent information regulator 2 homologous protein 1 SP - 140 EP - 5 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 43 IS - 3 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) combined with Gastrodin on learning-memory ability and expression of silent information regulator 2 homologous protein 1(SIRT 1) and peroxisome proliferator activated receptor γ coactivator (PGC-1 ɑ) of hippocampal CA 1 region in Alzheimer's disease(AD) rats, so as to explore its mechanism under-lying improvement of AD. METHODS: Sixty male SD rats were randomly divided into normal control (normal), sham operation (sham), model, EA, Gastrodin and EA+ Gastrodin groups (n=10 in each). The AD model was established by intraperitoneal injection of D-Galactose (120 mg•kg-1•d-1) combined with bilateral hippocampal injection of β amyloid 1-40(Aβ 1-40). EA was applied at "Baihui"(GV 20), "Dazhui"(GV 14) and "Zusanli"(ST 36) for 30 min, once daily for 4 weeks. For rats of the Gastro-din group and EA+ Gastrodin group, intraperitoneal injection of gastrodin(10 mg/kg) was conducted once daily for 4 weeks. Morris water maze tests were used to assess the rat's learning-memory ability. Nissl staining was used to assess the morphological changes of neurons in the hippocampal CA 1 area. The expression of SIRT 1 and PGC-1 ɑ of hippocampal CA 1 region was mea-sured by immunohistochemical staining. RESULTS: 1) Morris water maze tests showed that, compared with the normal and sham group, the escape latency was significantly prolonged (P<0.05), and the percentage of platform quadrant residence duration and the platform crossing times were considerably decreased in the model group (P<0.05). After the intervention, the escape latency was obviously shortened (P<0.05), and the percentage of platform quadrant residence duration and the platform crossing times were markedly increased in the EA, Gastrodin and EA+Gastrodin groups relevant to the model group (P<0.05). 2) Nissl staining showed that, in comparison with the normal group or sham group, the number of cells in the hippocampal CA 1 area was decreased and the arrangement was disorganized in the model group. The number of cells in CA 1 area was relatively higher in the 3 treatment groups than in the model group. 3) The expression levels of SIRT 1 and PGC-1 ɑ proteins in the hippocampal CA 1 area were significantly down-regulated in the model group than in the normal and sham groups (P<0.05). After the intervention, the expression levels of SIRT 1 and PGC-1 ɑ in the EA, Gastrodin and EA+Gastrodin groups were significantly up-regulated compared with the model group (P<0.05). The effects of EA+Gastrodin were significantly superior to those of simple EA and simple Gastrodin in shortening the escape latency, up-regulating the expression levels of SIRT 1 and PGC-1 ɑ as well as in increasing the percentage of platform quadrant residence time and platform crossing times (P<0.05). CONCLUSION: Both EA and Gastrodin can improve the learning-memory ability of AD rats, which may be related to their effects in up-regulating the expression of SIRT 1 and PGC-1 ɑ and reducing neuronal injury in the CA 1 region of hippocampus, suggesting a protective role of EA on hippocampal neurons. The effect of EA combined with Gastrodin is markedly better than that of EA and Gastrodin alone. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/29560628/[Electroacupuncture_plus_Gastrodin_Improves_Learning_memory_Ability_Possibly_by_Up_regulating_Expression_of_SIRT_1_and_PGC_1_ɑ_in_Hippocampal_CA_1_Region_of_Alzheimer's_Disease_Rats]_ L2 - https://medlineplus.gov/alzheimersdisease.html DB - PRIME DP - Unbound Medicine ER -