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[Effect of Electroacupuncture at "Neiguan"(PC 6) on Serum and Myocardial Metabolites in Rats with Myocardial Ischemia Reperfusion Injury Based on Nuclear Magnetic Resonance Spectroscopy].
Zhen Ci Yan Jiu. 2018 Mar 25; 43(3):152-62.ZC

Abstract

OBJECTIVE

We have repeatedly demonstrated that electroacupuncture (EA) of "Neiguan"(PC 6) can improve myocardial ischemia in rats. The present study was designed to investigate the metabolomic profile of peripheral blood se-rum and myocardium involving EA-induced improvement of myocardial ischemia-reperfusion injury (MIRI) in rats by using nuclear magnetic resonance spectroscopy.

METHODS

Thirty male SD rats were equally randomized into blank control, model and EA groups. Rats of the control group were only banded for 20 min, once a day for 7 days. The MIRI model was established by occlusion of the anterior descending branch of the left coronary artery for 40 min, followed by reperfusion for 60 min, and rats of the model group were banded as those in the control group. EA (10 Hz/50 Hz, 1 mA) was applied to bilateral PC 6 for 20 min, once daily for 7 days. The blood samples and left ventricular myocardial tissues were collected for assaying the profiles of differential metabolites using 1H nuclear magnetic resonance (1H NMR) spectroscopy and multivariate statistical analysis such as the principal components analysis (PCA), partial least squares-discriminant analysis (PLS-DA) and orthogonal PLS-DA (O-PLS-DA) with SIMCA-P software 12.0.

RESULTS

A total of 19 differential metabolites (17 down-regulated, 2 up-regulated) in the serum and 14 differential metabolites (13 down-regulated and 1 up-regulated) in the ischemic left myocardium were identified after MIRI. Of the 19 serum differential metabolites, amino acids (leucine, isoleucine, valine,alanine, lysine, glycine, glutamine), 3-hydroxy butyric acid (3-HB), lactic acid, acetate, N-acetyl glycoprotein (NAc), acetone, acetoacetate, succinate, polyunsaturated fatty acids (PUFA), creatine, glycerophosphocholine (GPC) were down-regulated; while low density lipoprotein (LDL), LDL/very low density lipoprotein(LDL/VLDL)and glucose obviously up-regulated. Of the 14 myocardial differential metabolites, amino acids (alanine, lysine, glutamate, glutamine, aspartate, taurine, glycine, threonine), GPC, creatine, lactic acid, adenosine monophosphate (AMP), nicotinamide adenine dinucleotide (NAD+) were significantly decreased, and glucose was up-regulated. Following EA treatment, most of the decreased serum differential metabolites except acetone, acetoacetate and PUFA, and the increased serum LDL, LDL/VLDL and glucose recovered, basically close to the control level; and the decreased myocardial creatine, GPC and NAD+ were also apparently up-regulated and the increased myocardial glucose was down-regulated. But, myocardial threonine and AMP still presented a decreasing state. Although the pattern of myocardial differential metabolites of the EA group had a trend to be close to the control group, the significant difference still existed, while the metabolic pattern of serum metabolites in the EA group was close to that of the control group.

CONCLUSION

EA stimulation of PC 6 can regulate serum or/and myocardial metabolites as amino acids, carbohydrates, lipids, etc. in MIRI rats, of which both serum and myocardial creatine, GPC and glucose may be jointly confer a favorable potential for EA-induced improvement of MIRI.

Authors+Show Affiliations

College of Acupuncture-moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha 410007, China.College of Acupuncture-moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha 410007, China.College of Acupuncture-moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha 410007, China.College of Acupuncture-moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha 410007, China.College of Acupuncture-moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha 410007, China.College of Acupuncture-moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha 410007, China.College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian Province.Department of Physical Education, Xiamen University of Technology, Xiamen 361005.College of Acupuncture-moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha 410007, China.College of Acupuncture-moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha 410007, China.College of Acupuncture-moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha 410007, China.College of Acupuncture-moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha 410007, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

29560630

Citation

Tang, Ya-Ni, et al. "[Effect of Electroacupuncture at "Neiguan"(PC 6) On Serum and Myocardial Metabolites in Rats With Myocardial Ischemia Reperfusion Injury Based On Nuclear Magnetic Resonance Spectroscopy]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 43, no. 3, 2018, pp. 152-62.
Tang YN, Tan CF, Liu WW, et al. [Effect of Electroacupuncture at "Neiguan"(PC 6) on Serum and Myocardial Metabolites in Rats with Myocardial Ischemia Reperfusion Injury Based on Nuclear Magnetic Resonance Spectroscopy]. Zhen Ci Yan Jiu. 2018;43(3):152-62.
Tang, Y. N., Tan, C. F., Liu, W. W., Yan, J., Wang, C., Liu, M., Lin, D. H., Huang, C. H., Du, L., Chen, M. L., Li, J. L., & Zhu, D. M. (2018). [Effect of Electroacupuncture at "Neiguan"(PC 6) on Serum and Myocardial Metabolites in Rats with Myocardial Ischemia Reperfusion Injury Based on Nuclear Magnetic Resonance Spectroscopy]. Zhen Ci Yan Jiu = Acupuncture Research, 43(3), 152-62. https://doi.org/10.13702/j.1000-0607.170436
Tang YN, et al. [Effect of Electroacupuncture at "Neiguan"(PC 6) On Serum and Myocardial Metabolites in Rats With Myocardial Ischemia Reperfusion Injury Based On Nuclear Magnetic Resonance Spectroscopy]. Zhen Ci Yan Jiu. 2018 Mar 25;43(3):152-62. PubMed PMID: 29560630.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of Electroacupuncture at "Neiguan"(PC 6) on Serum and Myocardial Metabolites in Rats with Myocardial Ischemia Reperfusion Injury Based on Nuclear Magnetic Resonance Spectroscopy]. AU - Tang,Ya-Ni, AU - Tan,Cheng-Fu, AU - Liu,Wei-Wei, AU - Yan,Jie, AU - Wang,Chao, AU - Liu,Mi, AU - Lin,Dong-Hai, AU - Huang,Cai-Hua, AU - Du,Lin, AU - Chen,Mei-Lin, AU - Li,Jiao-Lan, AU - Zhu,Ding-Ming, PY - 2018/3/22/entrez PY - 2018/3/22/pubmed PY - 2019/4/6/medline KW - Differential metabolites KW - Electroacupuncture KW - Left ventricle myocardium KW - Metabonomics KW - Myocardial ischemia reperfusion injury KW - Nuclear magnetic resonance KW - Serum SP - 152 EP - 62 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 43 IS - 3 N2 - OBJECTIVE: We have repeatedly demonstrated that electroacupuncture (EA) of "Neiguan"(PC 6) can improve myocardial ischemia in rats. The present study was designed to investigate the metabolomic profile of peripheral blood se-rum and myocardium involving EA-induced improvement of myocardial ischemia-reperfusion injury (MIRI) in rats by using nuclear magnetic resonance spectroscopy. METHODS: Thirty male SD rats were equally randomized into blank control, model and EA groups. Rats of the control group were only banded for 20 min, once a day for 7 days. The MIRI model was established by occlusion of the anterior descending branch of the left coronary artery for 40 min, followed by reperfusion for 60 min, and rats of the model group were banded as those in the control group. EA (10 Hz/50 Hz, 1 mA) was applied to bilateral PC 6 for 20 min, once daily for 7 days. The blood samples and left ventricular myocardial tissues were collected for assaying the profiles of differential metabolites using 1H nuclear magnetic resonance (1H NMR) spectroscopy and multivariate statistical analysis such as the principal components analysis (PCA), partial least squares-discriminant analysis (PLS-DA) and orthogonal PLS-DA (O-PLS-DA) with SIMCA-P software 12.0. RESULTS: A total of 19 differential metabolites (17 down-regulated, 2 up-regulated) in the serum and 14 differential metabolites (13 down-regulated and 1 up-regulated) in the ischemic left myocardium were identified after MIRI. Of the 19 serum differential metabolites, amino acids (leucine, isoleucine, valine,alanine, lysine, glycine, glutamine), 3-hydroxy butyric acid (3-HB), lactic acid, acetate, N-acetyl glycoprotein (NAc), acetone, acetoacetate, succinate, polyunsaturated fatty acids (PUFA), creatine, glycerophosphocholine (GPC) were down-regulated; while low density lipoprotein (LDL), LDL/very low density lipoprotein(LDL/VLDL)and glucose obviously up-regulated. Of the 14 myocardial differential metabolites, amino acids (alanine, lysine, glutamate, glutamine, aspartate, taurine, glycine, threonine), GPC, creatine, lactic acid, adenosine monophosphate (AMP), nicotinamide adenine dinucleotide (NAD+) were significantly decreased, and glucose was up-regulated. Following EA treatment, most of the decreased serum differential metabolites except acetone, acetoacetate and PUFA, and the increased serum LDL, LDL/VLDL and glucose recovered, basically close to the control level; and the decreased myocardial creatine, GPC and NAD+ were also apparently up-regulated and the increased myocardial glucose was down-regulated. But, myocardial threonine and AMP still presented a decreasing state. Although the pattern of myocardial differential metabolites of the EA group had a trend to be close to the control group, the significant difference still existed, while the metabolic pattern of serum metabolites in the EA group was close to that of the control group. CONCLUSION: EA stimulation of PC 6 can regulate serum or/and myocardial metabolites as amino acids, carbohydrates, lipids, etc. in MIRI rats, of which both serum and myocardial creatine, GPC and glucose may be jointly confer a favorable potential for EA-induced improvement of MIRI. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/29560630/[Effect_of_Electroacupuncture_at_"Neiguan"_PC_6__on_Serum_and_Myocardial_Metabolites_in_Rats_with_Myocardial_Ischemia_Reperfusion_Injury_Based_on_Nuclear_Magnetic_Resonance_Spectroscopy]_ DB - PRIME DP - Unbound Medicine ER -