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High-throughput genotyping-by-sequencing facilitates molecular tagging of a novel rust resistance gene, R 15 , in sunflower (Helianthus annuus L.).
Theor Appl Genet. 2018 Jul; 131(7):1423-1432.TA

Abstract

KEY MESSAGE

A novel rust resistance gene, R 15 , derived from the cultivated sunflower HA-R8 was assigned to linkage group 8 of the sunflower genome using a genotyping-by-sequencing approach. SNP markers closely linked to R 15 were identified, facilitating marker-assisted selection of resistance genes. The rust virulence gene is co-evolving with the resistance gene in sunflower, leading to the emergence of new physiologic pathotypes. This presents a continuous threat to the sunflower crop necessitating the development of resistant sunflower hybrids providing a more efficient, durable, and environmentally friendly host plant resistance. The inbred line HA-R8 carries a gene conferring resistance to all known races of the rust pathogen in North America and can be used as a broad-spectrum resistance resource. Based on phenotypic assessments of 140 F2 individuals derived from a cross of HA 89 with HA-R8, rust resistance in the population was found to be conferred by a single dominant gene (R 15) originating from HA-R8. Genotypic analysis with the currently available SSR markers failed to find any association between rust resistance and any markers. Therefore, we used genotyping-by-sequencing (GBS) analysis to achieve better genomic coverage. The GBS data showed that R 15 was located at the top end of linkage group (LG) 8. Saturation with 71 previously mapped SNP markers selected within this region further showed that it was located in a resistance gene cluster on LG8, and mapped to a 1.0-cM region between three co-segregating SNP makers SFW01920, SFW00128, and SFW05824 as well as the NSA_008457 SNP marker. These closely linked markers will facilitate marker-assisted selection and breeding in sunflower.

Authors+Show Affiliations

Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108, USA.Soybean Genomics and Improvement Laboratory, USDA-Agricultural Research Service, Beltsville, MD, 20705-2350, USA.Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108, USA.Red River Valley Agricultural Research Center, USDA-Agricultural Research Service, Fargo, ND, 58102-2765, USA. lili.qi@ars.usda.gov.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29564500

Citation

Ma, G J., et al. "High-throughput Genotyping-by-sequencing Facilitates Molecular Tagging of a Novel Rust Resistance Gene, R 15 , in Sunflower (Helianthus Annuus L.)." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 131, no. 7, 2018, pp. 1423-1432.
Ma GJ, Song QJ, Markell SG, et al. High-throughput genotyping-by-sequencing facilitates molecular tagging of a novel rust resistance gene, R 15 , in sunflower (Helianthus annuus L.). Theor Appl Genet. 2018;131(7):1423-1432.
Ma, G. J., Song, Q. J., Markell, S. G., & Qi, L. L. (2018). High-throughput genotyping-by-sequencing facilitates molecular tagging of a novel rust resistance gene, R 15 , in sunflower (Helianthus annuus L.). TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 131(7), 1423-1432. https://doi.org/10.1007/s00122-018-3087-5
Ma GJ, et al. High-throughput Genotyping-by-sequencing Facilitates Molecular Tagging of a Novel Rust Resistance Gene, R 15 , in Sunflower (Helianthus Annuus L.). Theor Appl Genet. 2018;131(7):1423-1432. PubMed PMID: 29564500.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-throughput genotyping-by-sequencing facilitates molecular tagging of a novel rust resistance gene, R 15 , in sunflower (Helianthus annuus L.). AU - Ma,G J, AU - Song,Q J, AU - Markell,S G, AU - Qi,L L, Y1 - 2018/03/21/ PY - 2017/10/24/received PY - 2018/03/12/accepted PY - 2018/3/23/pubmed PY - 2018/8/23/medline PY - 2018/3/23/entrez SP - 1423 EP - 1432 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor. Appl. Genet. VL - 131 IS - 7 N2 - KEY MESSAGE: A novel rust resistance gene, R 15 , derived from the cultivated sunflower HA-R8 was assigned to linkage group 8 of the sunflower genome using a genotyping-by-sequencing approach. SNP markers closely linked to R 15 were identified, facilitating marker-assisted selection of resistance genes. The rust virulence gene is co-evolving with the resistance gene in sunflower, leading to the emergence of new physiologic pathotypes. This presents a continuous threat to the sunflower crop necessitating the development of resistant sunflower hybrids providing a more efficient, durable, and environmentally friendly host plant resistance. The inbred line HA-R8 carries a gene conferring resistance to all known races of the rust pathogen in North America and can be used as a broad-spectrum resistance resource. Based on phenotypic assessments of 140 F2 individuals derived from a cross of HA 89 with HA-R8, rust resistance in the population was found to be conferred by a single dominant gene (R 15) originating from HA-R8. Genotypic analysis with the currently available SSR markers failed to find any association between rust resistance and any markers. Therefore, we used genotyping-by-sequencing (GBS) analysis to achieve better genomic coverage. The GBS data showed that R 15 was located at the top end of linkage group (LG) 8. Saturation with 71 previously mapped SNP markers selected within this region further showed that it was located in a resistance gene cluster on LG8, and mapped to a 1.0-cM region between three co-segregating SNP makers SFW01920, SFW00128, and SFW05824 as well as the NSA_008457 SNP marker. These closely linked markers will facilitate marker-assisted selection and breeding in sunflower. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/29564500/High_throughput_genotyping_by_sequencing_facilitates_molecular_tagging_of_a_novel_rust_resistance_gene_R_15__in_sunflower__Helianthus_annuus_L___ L2 - https://dx.doi.org/10.1007/s00122-018-3087-5 DB - PRIME DP - Unbound Medicine ER -