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LML1, Encoding a Conserved Eukaryotic Release Factor 1 Protein, Regulates Cell Death and Pathogen Resistance by Forming a Conserved Complex with SPL33 in Rice.
Plant Cell Physiol. 2018 May 01; 59(5):887-902.PC

Abstract

Lesion mimic mutants are powerful tools for unveiling the molecular connections between cell death and pathogen resistance. Various proteins responsible for lesion mimics have been identified; however, the mechanisms underlying lesion formation and pathogen resistance are still unknown. Here, we identify a lesion mimic mutant in rice, lesion mimic leaf 1 (lml1). The lml1 mutant exhibited abnormal cell death and resistance to both bacterial blight and rice blast. LML1 is expressed in all types of leaf cells, and encodes a novel eukaryotic release factor 1 (eRF1) protein located in the endoplasmic reticulum. Protein sequences of LML1 orthologs are conserved in yeast, animals and plants. LML1 can partially rescue the growth delay phenotype of the LML1 yeast ortholog mutant, dom34. Both lml1 and mutants of AtLML1 (the LML1 Arabidopsis ortholog) exhibited a growth delay phenotype like dom34. This indicates that LML1 and its orthologs are functionally conserved. LML1 forms a functional complex with a eukaryotic elongation factor 1A (eEF1A)-like protein, SPL33/LMM5.1, whose mutant phenotype was similar to the lml1 phenotype. This complex was conserved between rice and yeast. Our work provides new insight into understanding the mechanism of cell death and pathogen resistance, and also lays a good foundation for studying the fundamental molecular function of Pelota/DOM34 and its orthologs in plants.

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Authors+Show Affiliations

Qin P
Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan 611130, China. State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu Wenjiang, Sichuan 611130, China.
Fan S
Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan 611130, China. State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu Wenjiang, Sichuan 611130, China.
Deng L
Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan 611130, China. Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, 610066, China.
Zhong G
Hybrid Rice Research Center of Neijiang Academy of Agricultural, Neijiang, Sichuan 641000, China.
Zhang S
Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan 611130, China.
Li M
Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan 611130, China.
Chen W
Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan 611130, China.
Wang G
Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan 611130, China. State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu Wenjiang, Sichuan 611130, China.
Tu B
Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan 611130, China. State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu Wenjiang, Sichuan 611130, China.
Wang Y
Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan 611130, China.
Chen X
Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan 611130, China.
Ma B
Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan 611130, China. State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu Wenjiang, Sichuan 611130, China.
Li S
Rice Research Institute of Sichuan Agricultural University, Chengdu Wenjiang, Sichuan 611130, China. State Key Laboratory of Hybrid Rice, Sichuan Agricultural University, Chengdu Wenjiang, Sichuan 611130, China.

MeSH

Amino Acid SequenceCell DeathChloroplastsChromosome MappingCircadian ClocksCloning, MolecularConserved SequenceDisease ResistanceGene Expression Regulation, PlantLightMagnaportheOryzaPhenotypePhotoperiodPhylogenyPlant DiseasesPlant LeavesPlant ProteinsProtein BindingTemperature

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29566164

Citation

Qin, Peng, et al. "LML1, Encoding a Conserved Eukaryotic Release Factor 1 Protein, Regulates Cell Death and Pathogen Resistance By Forming a Conserved Complex With SPL33 in Rice." Plant & Cell Physiology, vol. 59, no. 5, 2018, pp. 887-902.
Qin P, Fan S, Deng L, et al. LML1, Encoding a Conserved Eukaryotic Release Factor 1 Protein, Regulates Cell Death and Pathogen Resistance by Forming a Conserved Complex with SPL33 in Rice. Plant Cell Physiol. 2018;59(5):887-902.
Qin, P., Fan, S., Deng, L., Zhong, G., Zhang, S., Li, M., Chen, W., Wang, G., Tu, B., Wang, Y., Chen, X., Ma, B., & Li, S. (2018). LML1, Encoding a Conserved Eukaryotic Release Factor 1 Protein, Regulates Cell Death and Pathogen Resistance by Forming a Conserved Complex with SPL33 in Rice. Plant & Cell Physiology, 59(5), 887-902. https://doi.org/10.1093/pcp/pcy056
Qin P, et al. LML1, Encoding a Conserved Eukaryotic Release Factor 1 Protein, Regulates Cell Death and Pathogen Resistance By Forming a Conserved Complex With SPL33 in Rice. Plant Cell Physiol. 2018 May 1;59(5):887-902. PubMed PMID: 29566164.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - LML1, Encoding a Conserved Eukaryotic Release Factor 1 Protein, Regulates Cell Death and Pathogen Resistance by Forming a Conserved Complex with SPL33 in Rice. AU - Qin,Peng, AU - Fan,Shijun, AU - Deng,Luchang, AU - Zhong,Guangrong, AU - Zhang,Siwei, AU - Li,Meng, AU - Chen,Weilan, AU - Wang,Geling, AU - Tu,Bin, AU - Wang,Yuping, AU - Chen,Xuewei, AU - Ma,Bingtian, AU - Li,Shigui, PY - 2017/12/12/received PY - 2018/03/09/accepted PY - 2018/3/23/pubmed PY - 2018/9/27/medline PY - 2018/3/23/entrez SP - 887 EP - 902 JF - Plant & cell physiology JO - Plant Cell Physiol VL - 59 IS - 5 N2 - Lesion mimic mutants are powerful tools for unveiling the molecular connections between cell death and pathogen resistance. Various proteins responsible for lesion mimics have been identified; however, the mechanisms underlying lesion formation and pathogen resistance are still unknown. Here, we identify a lesion mimic mutant in rice, lesion mimic leaf 1 (lml1). The lml1 mutant exhibited abnormal cell death and resistance to both bacterial blight and rice blast. LML1 is expressed in all types of leaf cells, and encodes a novel eukaryotic release factor 1 (eRF1) protein located in the endoplasmic reticulum. Protein sequences of LML1 orthologs are conserved in yeast, animals and plants. LML1 can partially rescue the growth delay phenotype of the LML1 yeast ortholog mutant, dom34. Both lml1 and mutants of AtLML1 (the LML1 Arabidopsis ortholog) exhibited a growth delay phenotype like dom34. This indicates that LML1 and its orthologs are functionally conserved. LML1 forms a functional complex with a eukaryotic elongation factor 1A (eEF1A)-like protein, SPL33/LMM5.1, whose mutant phenotype was similar to the lml1 phenotype. This complex was conserved between rice and yeast. Our work provides new insight into understanding the mechanism of cell death and pathogen resistance, and also lays a good foundation for studying the fundamental molecular function of Pelota/DOM34 and its orthologs in plants. SN - 1471-9053 UR - https://www.unboundmedicine.com/medline/citation/29566164/LML1_Encoding_a_Conserved_Eukaryotic_Release_Factor_1_Protein_Regulates_Cell_Death_and_Pathogen_Resistance_by_Forming_a_Conserved_Complex_with_SPL33_in_Rice_ L2 - https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcy056 DB - PRIME DP - Unbound Medicine ER -