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Downregulation of lncRNA H19 inhibits the migration and invasion of melanoma cells by inactivating the NF‑κB and PI3K/Akt signaling pathways.
Mol Med Rep 2018; 17(5):7313-7318MM

Abstract

As the most aggressive type of skin cancer, melanoma seriously affects human health. Long noncoding (lncRNA) 19 has been demonstrated to be involved in the progression of a number of different types of human cancers. However, the involvement of lncRNA H19 in melanoma remains unknown. Therefore, the present study was performed to investigate the roles of H19 in the development and progression of melanoma. In the present study, 49 patients with melanoma were included. Expression of lncRNA H19 in tumor tissue, adjacent healthy tissue and various cell lines with different treatments was measured by reverse transcription‑quantitative polymerase chain reaction. The effects of H19 knockdown on melanoma cell proliferation, migration and invasion were detected by cell counting kit‑8, wound‑healing and transwell invasion assays, respectively. In addition, the effects of H19 knockdown on the expression of nuclear factor (NF)‑κB pathway‑associated proteins were investigated by western blotting. The results revealed that the expression level of H19 was significantly higher in tumor tissue than in the adjacent healthy tissue of 47 out of 49 patients. H19 knockdown significantly reduced the proliferation, migration and invasion ability of melanoma cells. H19 knockdown also inactivated the phosphoinositide 3‑kinase (PI3K)/protein kinase B (Akt) pathway, which in turn inhibited the activation of the NF‑κB signaling pathway. Thus, downregulation of lncRNA H19 may inhibit the migration and invasion of melanoma cells by inactivating the NF‑κB signaling pathway via the inactivation of the PI3K/Akt signaling pathway. The present study provided references for future studies on the pathogenesis of melanoma and the clinical treatment of this disease.

Authors+Show Affiliations

Department of Bone and Soft Tissue Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin 300060, P.R. China.Department of Bone and Soft Tissue Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin 300060, P.R. China.Department of Bone and Soft Tissue Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin 300060, P.R. China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29568965

Citation

Liao, Zhichao, et al. "Downregulation of lncRNA H19 Inhibits the Migration and Invasion of Melanoma Cells By Inactivating the NF‑κB and PI3K/Akt Signaling Pathways." Molecular Medicine Reports, vol. 17, no. 5, 2018, pp. 7313-7318.
Liao Z, Zhao J, Yang Y. Downregulation of lncRNA H19 inhibits the migration and invasion of melanoma cells by inactivating the NF‑κB and PI3K/Akt signaling pathways. Mol Med Rep. 2018;17(5):7313-7318.
Liao, Z., Zhao, J., & Yang, Y. (2018). Downregulation of lncRNA H19 inhibits the migration and invasion of melanoma cells by inactivating the NF‑κB and PI3K/Akt signaling pathways. Molecular Medicine Reports, 17(5), pp. 7313-7318. doi:10.3892/mmr.2018.8782.
Liao Z, Zhao J, Yang Y. Downregulation of lncRNA H19 Inhibits the Migration and Invasion of Melanoma Cells By Inactivating the NF‑κB and PI3K/Akt Signaling Pathways. Mol Med Rep. 2018;17(5):7313-7318. PubMed PMID: 29568965.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Downregulation of lncRNA H19 inhibits the migration and invasion of melanoma cells by inactivating the NF‑κB and PI3K/Akt signaling pathways. AU - Liao,Zhichao, AU - Zhao,Jun, AU - Yang,Yun, Y1 - 2018/03/20/ PY - 2017/09/29/received PY - 2018/01/02/accepted PY - 2018/3/24/pubmed PY - 2018/9/22/medline PY - 2018/3/24/entrez SP - 7313 EP - 7318 JF - Molecular medicine reports JO - Mol Med Rep VL - 17 IS - 5 N2 - As the most aggressive type of skin cancer, melanoma seriously affects human health. Long noncoding (lncRNA) 19 has been demonstrated to be involved in the progression of a number of different types of human cancers. However, the involvement of lncRNA H19 in melanoma remains unknown. Therefore, the present study was performed to investigate the roles of H19 in the development and progression of melanoma. In the present study, 49 patients with melanoma were included. Expression of lncRNA H19 in tumor tissue, adjacent healthy tissue and various cell lines with different treatments was measured by reverse transcription‑quantitative polymerase chain reaction. The effects of H19 knockdown on melanoma cell proliferation, migration and invasion were detected by cell counting kit‑8, wound‑healing and transwell invasion assays, respectively. In addition, the effects of H19 knockdown on the expression of nuclear factor (NF)‑κB pathway‑associated proteins were investigated by western blotting. The results revealed that the expression level of H19 was significantly higher in tumor tissue than in the adjacent healthy tissue of 47 out of 49 patients. H19 knockdown significantly reduced the proliferation, migration and invasion ability of melanoma cells. H19 knockdown also inactivated the phosphoinositide 3‑kinase (PI3K)/protein kinase B (Akt) pathway, which in turn inhibited the activation of the NF‑κB signaling pathway. Thus, downregulation of lncRNA H19 may inhibit the migration and invasion of melanoma cells by inactivating the NF‑κB signaling pathway via the inactivation of the PI3K/Akt signaling pathway. The present study provided references for future studies on the pathogenesis of melanoma and the clinical treatment of this disease. SN - 1791-3004 UR - https://www.unboundmedicine.com/medline/citation/29568965/Downregulation_of_lncRNA_H19_inhibits_the_migration_and_invasion_of_melanoma_cells_by_inactivating_the_NF‑κB_and_PI3K/Akt_signaling_pathways_ L2 - http://www.spandidos-publications.com/mmr/17/5/7313 DB - PRIME DP - Unbound Medicine ER -