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Hepatic Lipogenesis and Brain Fatty Acid Profile in Response to Different Dietary n6/n3 Ratios and DHA/EPA Supplementation in Streptozotocin Treated Rats.
Mol Nutr Food Res 2018; 62(9):e1701007MN

Abstract

SCOPE

We investigated the interaction between streptozotocin (STZ)-induced diabetes and dietary n6/n3 ratio, and its influence on lipogenesis.

METHODS AND RESULTS

The animals were treated with STZ and fed with different dietary n6/n3 ratios: 1, 7, and 60, or supplemented with DHA/EPA. Gene expression was assessed by RT-PCR and protein expression by western blotting and immunohistochemistry. Fatty acid profile was determined by GC-MS. Pancreas and liver histology were assessed by hematoxylin and eosin (H&E) staining. STZ-induced characteristic changes in all STZ treated groups, including: increased blood glucose, decreased body mass, increased lipid peroxidation and CD36 expression, decreased 16:1n7 and 18:1n7, increases in 20:3n6, decreases in phospholipid (PL) content of 20:4n6, as well as decreases in the expression of SREBP1c, Δ-9-desaturase (Δ9D), and Δ-5-desaturase (Δ5D). Additionally, other changes occurred that were dependent on the n6/n3 ratio. Among the diabetic groups, the lower n6/n3 ratio caused higher lipid peroxidation and CD36 expression, a greater decrease in 20:4n6 and decreased Δ6-desaturase (Δ6D) expression, while the higher n6/n3 ratio caused increased partitioning of 20:4n6 into hepatic neutral lipids (NL), a decrease in 20:5n3 content, and increased β-oxidation.

CONCLUSION

Presented data suggest that the n6/n3 ratio could significantly influence lipogenesis, lipid peroxidation, and β-oxidation in STZ-induced diabetes, which could have clinical significance.

Authors+Show Affiliations

Department of Animal Husbandry, University of Zagreb, Faculty of Veterinary Medicine, 10000, Zagreb, Croatia.Department of Anatomy, Histology and Embryology, University of Split, School of Medicine, 21000, Split, Croatia.VetMedZg ERA Chair project, University of Zagreb, Faculty of Veterinary Medicine, 10000, Zagreb, Croatia.Institute for Medical Research and Occupational Health, 10000, Zagreb, Croatia.Department of Veterinary Pathology, University of Zagreb, Faculty of Veterinary Medicine, 10000, Zagreb, Croatia.Department of Animal Nutrition and Dietetics, University of Zagreb, Faculty of Veterinary Medicine, 10000, Zagreb, Croatia.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29579359

Citation

Starčević, Kristina, et al. "Hepatic Lipogenesis and Brain Fatty Acid Profile in Response to Different Dietary N6/n3 Ratios and DHA/EPA Supplementation in Streptozotocin Treated Rats." Molecular Nutrition & Food Research, vol. 62, no. 9, 2018, pp. e1701007.
Starčević K, Filipović N, Galan A, et al. Hepatic Lipogenesis and Brain Fatty Acid Profile in Response to Different Dietary n6/n3 Ratios and DHA/EPA Supplementation in Streptozotocin Treated Rats. Mol Nutr Food Res. 2018;62(9):e1701007.
Starčević, K., Filipović, N., Galan, A., Micek, V., Gudan Kurilj, A., & Mašek, T. (2018). Hepatic Lipogenesis and Brain Fatty Acid Profile in Response to Different Dietary n6/n3 Ratios and DHA/EPA Supplementation in Streptozotocin Treated Rats. Molecular Nutrition & Food Research, 62(9), pp. e1701007. doi:10.1002/mnfr.201701007.
Starčević K, et al. Hepatic Lipogenesis and Brain Fatty Acid Profile in Response to Different Dietary N6/n3 Ratios and DHA/EPA Supplementation in Streptozotocin Treated Rats. Mol Nutr Food Res. 2018;62(9):e1701007. PubMed PMID: 29579359.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepatic Lipogenesis and Brain Fatty Acid Profile in Response to Different Dietary n6/n3 Ratios and DHA/EPA Supplementation in Streptozotocin Treated Rats. AU - Starčević,Kristina, AU - Filipović,Natalija, AU - Galan,Asier, AU - Micek,Vedran, AU - Gudan Kurilj,Andrea, AU - Mašek,Tomislav, Y1 - 2018/04/20/ PY - 2017/12/04/received PY - 2018/03/07/revised PY - 2018/3/27/pubmed PY - 2019/1/17/medline PY - 2018/3/27/entrez KW - RAGE KW - delta-desaturases KW - diabetes type 1 KW - fatty acids KW - lipid oxidation KW - β-oxidation SP - e1701007 EP - e1701007 JF - Molecular nutrition & food research JO - Mol Nutr Food Res VL - 62 IS - 9 N2 - SCOPE: We investigated the interaction between streptozotocin (STZ)-induced diabetes and dietary n6/n3 ratio, and its influence on lipogenesis. METHODS AND RESULTS: The animals were treated with STZ and fed with different dietary n6/n3 ratios: 1, 7, and 60, or supplemented with DHA/EPA. Gene expression was assessed by RT-PCR and protein expression by western blotting and immunohistochemistry. Fatty acid profile was determined by GC-MS. Pancreas and liver histology were assessed by hematoxylin and eosin (H&E) staining. STZ-induced characteristic changes in all STZ treated groups, including: increased blood glucose, decreased body mass, increased lipid peroxidation and CD36 expression, decreased 16:1n7 and 18:1n7, increases in 20:3n6, decreases in phospholipid (PL) content of 20:4n6, as well as decreases in the expression of SREBP1c, Δ-9-desaturase (Δ9D), and Δ-5-desaturase (Δ5D). Additionally, other changes occurred that were dependent on the n6/n3 ratio. Among the diabetic groups, the lower n6/n3 ratio caused higher lipid peroxidation and CD36 expression, a greater decrease in 20:4n6 and decreased Δ6-desaturase (Δ6D) expression, while the higher n6/n3 ratio caused increased partitioning of 20:4n6 into hepatic neutral lipids (NL), a decrease in 20:5n3 content, and increased β-oxidation. CONCLUSION: Presented data suggest that the n6/n3 ratio could significantly influence lipogenesis, lipid peroxidation, and β-oxidation in STZ-induced diabetes, which could have clinical significance. SN - 1613-4133 UR - https://www.unboundmedicine.com/medline/citation/29579359/Hepatic_Lipogenesis_and_Brain_Fatty_Acid_Profile_in_Response_to_Different_Dietary_n6/n3_Ratios_and_DHA/EPA_Supplementation_in_Streptozotocin_Treated_Rats_ L2 - https://doi.org/10.1002/mnfr.201701007 DB - PRIME DP - Unbound Medicine ER -