TY - JOUR T1 - Non-destructive analysis of sucrose, caffeine and trigonelline on single green coffee beans by hyperspectral imaging. AU - Caporaso,Nicola, AU - Whitworth,Martin B, AU - Grebby,Stephen, AU - Fisk,Ian D, Y1 - 2017/12/14/ PY - 2017/09/13/received PY - 2017/11/20/revised PY - 2017/12/12/accepted PY - 2018/3/28/entrez PY - 2018/3/28/pubmed PY - 2019/9/24/medline KW - Caffeine KW - Coffee chemistry KW - Coffee sugars KW - Hyperspectral chemical imaging KW - NIR chemical mapping KW - Single seed variability SP - 193 EP - 203 JF - Food research international (Ottawa, Ont.) JO - Food Res Int VL - 106 N2 - Hyperspectral imaging (HSI) is a novel technology for the food sector that enables rapid non-contact analysis of food materials. HSI was applied for the first time to whole green coffee beans, at a single seed level, for quantitative prediction of sucrose, caffeine and trigonelline content. In addition, the intra-bean distribution of coffee constituents was analysed in Arabica and Robusta coffees on a large sample set from 12 countries, using a total of 260 samples. Individual green coffee beans were scanned by reflectance HSI (980-2500nm) and then the concentration of sucrose, caffeine and trigonelline analysed with a reference method (HPLC-MS). Quantitative prediction models were subsequently built using Partial Least Squares (PLS) regression. Large variations in sucrose, caffeine and trigonelline were found between different species and origin, but also within beans from the same batch. It was shown that estimation of sucrose content is possible for screening purposes (R2=0.65; prediction error of ~0.7% w/w coffee, with observed range of ~6.5%), while the performance of the PLS model was better for caffeine and trigonelline prediction (R2=0.85 and R2=0.82, respectively; prediction errors of 0.2 and 0.1%, on a range of 2.3 and 1.1% w/w coffee, respectively). The prediction error is acceptable mainly for laboratory applications, with the potential application to breeding programmes and for screening purposes for the food industry. The spatial distribution of coffee constituents was also successfully visualised for single beans and this enabled mapping of the analytes across the bean structure at single pixel level. SN - 1873-7145 UR - https://www.unboundmedicine.com/medline/citation/29579918/Non_destructive_analysis_of_sucrose_caffeine_and_trigonelline_on_single_green_coffee_beans_by_hyperspectral_imaging_ DB - PRIME DP - Unbound Medicine ER -